Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal

Detalhes bibliográficos
Autor(a) principal: Mesquita, João R.
Data de Publicação: 2016
Outros Autores: Oliveira, R. M. S., Coelho, Catarina, Vieira-Pinto, M., Nascimento, M. S. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.19/4664
Resumo: Hepatitis E virus (HEV) is a zoonotic agent today considered a major Public Health issue in industrialized countries. HEV strains belonging to zoonotic genotype 3 are widely present in swine, being today considered important reservoirs for human disease. Unlike in swine, only scarce data are available on the circulation of HEV in wild boar. This study describes the detection and molecular characterization of HEV in livers from sylvatic wild boar hunted in Portugal and destined for consumption. Additionally, the detection of HEV in stools of a confined wild boar population also destined for consumption is also described. A total of 80 liver samples collected during the hunting season of 2011/2012 and 40 stools collected in February 2012 from a wild boar breeding farm in Portugal were tested by a nested broad-spectrum RT-PCR assay targeting open reading frame (ORF) 1. Twenty livers (25.0%) and 4 stools (10%) were positive for HEV. Phylogenetic analysis showed that all strains clustered with sequences classified as HEV genotype 3 subgenotype e. To our knowledge, this is the first report documenting the occurrence and molecular analysis of HEV in sylvatic and captive wild boar destined for human consumption in Portugal. This report demonstrates for the first time the circulation of HEV in wildlife reservoirs of Portugal adding knowledge to the epidemiology of HEV in wild boar populations.
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spelling Hepatitis E Virus in Sylvatic and Captive Wild Boar from PortugalAnimalsWild AnimalsFecesGenotypeHepatitis EHepatitis E virusLiverPhylogenyPortugalSwineSwine DiseasesHepatitis E virus (HEV) is a zoonotic agent today considered a major Public Health issue in industrialized countries. HEV strains belonging to zoonotic genotype 3 are widely present in swine, being today considered important reservoirs for human disease. Unlike in swine, only scarce data are available on the circulation of HEV in wild boar. This study describes the detection and molecular characterization of HEV in livers from sylvatic wild boar hunted in Portugal and destined for consumption. Additionally, the detection of HEV in stools of a confined wild boar population also destined for consumption is also described. A total of 80 liver samples collected during the hunting season of 2011/2012 and 40 stools collected in February 2012 from a wild boar breeding farm in Portugal were tested by a nested broad-spectrum RT-PCR assay targeting open reading frame (ORF) 1. Twenty livers (25.0%) and 4 stools (10%) were positive for HEV. Phylogenetic analysis showed that all strains clustered with sequences classified as HEV genotype 3 subgenotype e. To our knowledge, this is the first report documenting the occurrence and molecular analysis of HEV in sylvatic and captive wild boar destined for human consumption in Portugal. This report demonstrates for the first time the circulation of HEV in wildlife reservoirs of Portugal adding knowledge to the epidemiology of HEV in wild boar populations.WileyRepositório Científico do Instituto Politécnico de ViseuMesquita, João R.Oliveira, R. M. S.Coelho, CatarinaVieira-Pinto, M.Nascimento, M. S. J.2017-10-03T00:30:11Z2016-10-012016-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://hdl.handle.net/10400.19/4664engMesquita, J. R., Oliveira, R. M. S., Coelho, C., Vieira-Pinto, M. & Nascimento, M. S. J. (2016). Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal. Transboundary and Emerging Diseases, 63 (5), 574–578. doi:10.1111/tbed.1229710.1111/tbed.12297info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-16T15:27:26Zoai:repositorio.ipv.pt:10400.19/4664Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:43:13.602136Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal
title Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal
spellingShingle Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal
Mesquita, João R.
Animals
Wild Animals
Feces
Genotype
Hepatitis E
Hepatitis E virus
Liver
Phylogeny
Portugal
Swine
Swine Diseases
title_short Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal
title_full Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal
title_fullStr Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal
title_full_unstemmed Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal
title_sort Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal
author Mesquita, João R.
author_facet Mesquita, João R.
Oliveira, R. M. S.
Coelho, Catarina
Vieira-Pinto, M.
Nascimento, M. S. J.
author_role author
author2 Oliveira, R. M. S.
Coelho, Catarina
Vieira-Pinto, M.
Nascimento, M. S. J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico de Viseu
dc.contributor.author.fl_str_mv Mesquita, João R.
Oliveira, R. M. S.
Coelho, Catarina
Vieira-Pinto, M.
Nascimento, M. S. J.
dc.subject.por.fl_str_mv Animals
Wild Animals
Feces
Genotype
Hepatitis E
Hepatitis E virus
Liver
Phylogeny
Portugal
Swine
Swine Diseases
topic Animals
Wild Animals
Feces
Genotype
Hepatitis E
Hepatitis E virus
Liver
Phylogeny
Portugal
Swine
Swine Diseases
description Hepatitis E virus (HEV) is a zoonotic agent today considered a major Public Health issue in industrialized countries. HEV strains belonging to zoonotic genotype 3 are widely present in swine, being today considered important reservoirs for human disease. Unlike in swine, only scarce data are available on the circulation of HEV in wild boar. This study describes the detection and molecular characterization of HEV in livers from sylvatic wild boar hunted in Portugal and destined for consumption. Additionally, the detection of HEV in stools of a confined wild boar population also destined for consumption is also described. A total of 80 liver samples collected during the hunting season of 2011/2012 and 40 stools collected in February 2012 from a wild boar breeding farm in Portugal were tested by a nested broad-spectrum RT-PCR assay targeting open reading frame (ORF) 1. Twenty livers (25.0%) and 4 stools (10%) were positive for HEV. Phylogenetic analysis showed that all strains clustered with sequences classified as HEV genotype 3 subgenotype e. To our knowledge, this is the first report documenting the occurrence and molecular analysis of HEV in sylvatic and captive wild boar destined for human consumption in Portugal. This report demonstrates for the first time the circulation of HEV in wildlife reservoirs of Portugal adding knowledge to the epidemiology of HEV in wild boar populations.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-01
2016-10-01T00:00:00Z
2017-10-03T00:30:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.19/4664
url http://hdl.handle.net/10400.19/4664
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mesquita, J. R., Oliveira, R. M. S., Coelho, C., Vieira-Pinto, M. & Nascimento, M. S. J. (2016). Hepatitis E Virus in Sylvatic and Captive Wild Boar from Portugal. Transboundary and Emerging Diseases, 63 (5), 574–578. doi:10.1111/tbed.12297
10.1111/tbed.12297
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
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dc.format.none.fl_str_mv application/vnd.openxmlformats-officedocument.wordprocessingml.document
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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