Analysis of surfaces for characterization of fungal burden
Main Author: | |
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Publication Date: | 2016 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | https://doi.org/10.13075/ijomeh.1896.00562 |
Summary: | Objectives: Mycological contamination of occupational environments can be a result of fungal spores' dispersion in the air and on surfaces. Therefore, it is very important to assess it in both types of the samples. In the present study we assessed fungal contamination in the air and in the surface samples to show relevance of surfaces sampling in complementing the results obtained in the air samples. Material and Methods: In total, 42 settings were assessed by the analysis of air and surfaces samples. The settings were divided into settings with a high fungal load (7 poultry farms and 7 pig farms, 3 cork industries, 3 waste management plants, 2 wastewater treatment plants and 1 horse stable) and a low fungal load (10 hospital canteens, 8 college canteens and 1 maternity hospital). In addition to culture-based methods, molecular tools were also applied to detect fungal burden in the settings with a higher fungal load. Results: From the 218 sampling sites, 140 (64.2%) presented different species in the examined surfaces when compared with the species identified in the air. A positive association in the high fungal load settings was found between the presence of different species in the air and surfaces. Wastewater treatment plants constituted the setting with the highest number of different species between the air and surface. Conclusions: We observed that surfaces sampling and application of molecular tools showed the same efficacy of species detection in high fungal load settings, corroborating the fact that surface sampling is crucial for a correct and complete analysis of occupational scenarios. |
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Analysis of surfaces for characterization of fungal burdenDoes it matter?Air samplesFungal burden assessmentHigh fungal load settingsLow fungal load settingsOccupational environmentsSurface samplesPublic Health, Environmental and Occupational HealthSDG 3 - Good Health and Well-beingObjectives: Mycological contamination of occupational environments can be a result of fungal spores' dispersion in the air and on surfaces. Therefore, it is very important to assess it in both types of the samples. In the present study we assessed fungal contamination in the air and in the surface samples to show relevance of surfaces sampling in complementing the results obtained in the air samples. Material and Methods: In total, 42 settings were assessed by the analysis of air and surfaces samples. The settings were divided into settings with a high fungal load (7 poultry farms and 7 pig farms, 3 cork industries, 3 waste management plants, 2 wastewater treatment plants and 1 horse stable) and a low fungal load (10 hospital canteens, 8 college canteens and 1 maternity hospital). In addition to culture-based methods, molecular tools were also applied to detect fungal burden in the settings with a higher fungal load. Results: From the 218 sampling sites, 140 (64.2%) presented different species in the examined surfaces when compared with the species identified in the air. A positive association in the high fungal load settings was found between the presence of different species in the air and surfaces. Wastewater treatment plants constituted the setting with the highest number of different species between the air and surface. Conclusions: We observed that surfaces sampling and application of molecular tools showed the same efficacy of species detection in high fungal load settings, corroborating the fact that surface sampling is crucial for a correct and complete analysis of occupational scenarios.Centro de Investigação em Saúde Pública (CISP/PHRC)RUNViegas, CarlaFaria, TiagoMeneses, MárciaCarolino, ElisabeteViegas, SusanaGomes, Anita QuintalSabino, Raquel2018-03-01T23:13:49Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttps://doi.org/10.13075/ijomeh.1896.00562eng1232-1087PURE: 3308975http://www.scopus.com/inward/record.url?scp=85015648198&partnerID=8YFLogxKhttps://doi.org/10.13075/ijomeh.1896.00562info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:17:29Zoai:run.unl.pt:10362/31533Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:29:41.336819Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Analysis of surfaces for characterization of fungal burden Does it matter? |
title |
Analysis of surfaces for characterization of fungal burden |
spellingShingle |
Analysis of surfaces for characterization of fungal burden Viegas, Carla Air samples Fungal burden assessment High fungal load settings Low fungal load settings Occupational environments Surface samples Public Health, Environmental and Occupational Health SDG 3 - Good Health and Well-being |
title_short |
Analysis of surfaces for characterization of fungal burden |
title_full |
Analysis of surfaces for characterization of fungal burden |
title_fullStr |
Analysis of surfaces for characterization of fungal burden |
title_full_unstemmed |
Analysis of surfaces for characterization of fungal burden |
title_sort |
Analysis of surfaces for characterization of fungal burden |
author |
Viegas, Carla |
author_facet |
Viegas, Carla Faria, Tiago Meneses, Márcia Carolino, Elisabete Viegas, Susana Gomes, Anita Quintal Sabino, Raquel |
author_role |
author |
author2 |
Faria, Tiago Meneses, Márcia Carolino, Elisabete Viegas, Susana Gomes, Anita Quintal Sabino, Raquel |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Centro de Investigação em Saúde Pública (CISP/PHRC) RUN |
dc.contributor.author.fl_str_mv |
Viegas, Carla Faria, Tiago Meneses, Márcia Carolino, Elisabete Viegas, Susana Gomes, Anita Quintal Sabino, Raquel |
dc.subject.por.fl_str_mv |
Air samples Fungal burden assessment High fungal load settings Low fungal load settings Occupational environments Surface samples Public Health, Environmental and Occupational Health SDG 3 - Good Health and Well-being |
topic |
Air samples Fungal burden assessment High fungal load settings Low fungal load settings Occupational environments Surface samples Public Health, Environmental and Occupational Health SDG 3 - Good Health and Well-being |
description |
Objectives: Mycological contamination of occupational environments can be a result of fungal spores' dispersion in the air and on surfaces. Therefore, it is very important to assess it in both types of the samples. In the present study we assessed fungal contamination in the air and in the surface samples to show relevance of surfaces sampling in complementing the results obtained in the air samples. Material and Methods: In total, 42 settings were assessed by the analysis of air and surfaces samples. The settings were divided into settings with a high fungal load (7 poultry farms and 7 pig farms, 3 cork industries, 3 waste management plants, 2 wastewater treatment plants and 1 horse stable) and a low fungal load (10 hospital canteens, 8 college canteens and 1 maternity hospital). In addition to culture-based methods, molecular tools were also applied to detect fungal burden in the settings with a higher fungal load. Results: From the 218 sampling sites, 140 (64.2%) presented different species in the examined surfaces when compared with the species identified in the air. A positive association in the high fungal load settings was found between the presence of different species in the air and surfaces. Wastewater treatment plants constituted the setting with the highest number of different species between the air and surface. Conclusions: We observed that surfaces sampling and application of molecular tools showed the same efficacy of species detection in high fungal load settings, corroborating the fact that surface sampling is crucial for a correct and complete analysis of occupational scenarios. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2018-03-01T23:13:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.13075/ijomeh.1896.00562 |
url |
https://doi.org/10.13075/ijomeh.1896.00562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1232-1087 PURE: 3308975 http://www.scopus.com/inward/record.url?scp=85015648198&partnerID=8YFLogxK https://doi.org/10.13075/ijomeh.1896.00562 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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10 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137921948712960 |