Wild mushroom extracts as inhibitors of bacterial biofilm formation

Detalhes bibliográficos
Autor(a) principal: Alves, Maria José
Data de Publicação: 2014
Outros Autores: Ferreira, Isabel C.F.R., Lourenço, Inês, Costa, Eduardo Manuel, Martins, Anabela, Pintado, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/12027
Resumo: Microorganisms can colonize a wide variety of medical devices, putting patients in risk for local and systemic infectious complications, including local-site infections, catheter-related bloodstream infections, and endocarditis. These microorganisms are able to grow adhered to almost every surface, forming architecturally complex communities termed biofilms. The use of natural products has been extremely successful in the discovery of new medicine, and mushrooms could be a source of natural antimicrobials. The present study reports the capacity of wild mushroom extracts to inhibit in vitro biofilm formation by multi-resistant bacteria. Four Gram-negative bacteria biofilm producers (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Acinetobacter baumannii) isolated from urine were used to verify the activity of Russula delica, Fistulina hepatica, Mycena rosea, Leucopaxilus giganteus, and Lepista nuda extracts. The results obtained showed that all tested mushroom extracts presented some extent of inhibition of biofilm production. Pseudomonas aeruginosa was the microorganism with the highest capacity of biofilm production, being also the most susceptible to the extracts inhibition capacity (equal or higher than 50%). Among the five tested extracts against E. coli, Leucopaxillus giganteus (47.8%) and Mycenas rosea (44.8%) presented the highest inhibition of biofilm formation. The extracts exhibiting the highest inhibitory effect upon P. mirabilis biofilm formation were Sarcodon imbricatus (45.4%) and Russula delica (53.1%). Acinetobacter baumannii was the microorganism with the lowest susceptibility to mushroom extracts inhibitory effect on biofilm production (highest inhibition—almost 29%, by Russula delica extract). This is a pioneer study since, as far as we know, there are no reports on the inhibition of biofilm production by the studied mushroom extracts and in particular against multi-resistant clinical isolates; nevertheless, other studies are required to elucidate the mechanism of action.
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spelling Wild mushroom extracts as inhibitors of bacterial biofilm formationClinical isolatesBiofilmWild mushroom extractsMulti-resistantCytotoxicityMicroorganisms can colonize a wide variety of medical devices, putting patients in risk for local and systemic infectious complications, including local-site infections, catheter-related bloodstream infections, and endocarditis. These microorganisms are able to grow adhered to almost every surface, forming architecturally complex communities termed biofilms. The use of natural products has been extremely successful in the discovery of new medicine, and mushrooms could be a source of natural antimicrobials. The present study reports the capacity of wild mushroom extracts to inhibit in vitro biofilm formation by multi-resistant bacteria. Four Gram-negative bacteria biofilm producers (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Acinetobacter baumannii) isolated from urine were used to verify the activity of Russula delica, Fistulina hepatica, Mycena rosea, Leucopaxilus giganteus, and Lepista nuda extracts. The results obtained showed that all tested mushroom extracts presented some extent of inhibition of biofilm production. Pseudomonas aeruginosa was the microorganism with the highest capacity of biofilm production, being also the most susceptible to the extracts inhibition capacity (equal or higher than 50%). Among the five tested extracts against E. coli, Leucopaxillus giganteus (47.8%) and Mycenas rosea (44.8%) presented the highest inhibition of biofilm formation. The extracts exhibiting the highest inhibitory effect upon P. mirabilis biofilm formation were Sarcodon imbricatus (45.4%) and Russula delica (53.1%). Acinetobacter baumannii was the microorganism with the lowest susceptibility to mushroom extracts inhibitory effect on biofilm production (highest inhibition—almost 29%, by Russula delica extract). This is a pioneer study since, as far as we know, there are no reports on the inhibition of biofilm production by the studied mushroom extracts and in particular against multi-resistant clinical isolates; nevertheless, other studies are required to elucidate the mechanism of action.The authors are grateful to Fundação para a Ciência e a Tecnologia (FCT, Portugal) and COMPETE/QREN/EU for financial support to strategic projects PEst-OE/AGR/UI0690/2011 and PEst-OE/EQB/LA0016/2011. They also thank CHTMAD—Hospital Center of Trás-os-Montes e Alto Douro and Siemens for all the support.Biblioteca Digital do IPBAlves, Maria JoséFerreira, Isabel C.F.R.Lourenço, InêsCosta, Eduardo ManuelMartins, AnabelaPintado, Manuela2015-08-03T11:05:46Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/12027engAlves, Maria José; Ferreira, Isabel C.F.R.; Lourenço, Inês; Costa, Eduardo; Martins, Anabela; Pintado, Manuela (2014). Wild mushroom extracts as inhibitors of bacterial biofilm formation. Pathogens. ISSN 2076-0817. 3:3, p. 667-6792076-081710.3390/pathogens3030667info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:28:11Zoai:bibliotecadigital.ipb.pt:10198/12027Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:02:27.295555Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Wild mushroom extracts as inhibitors of bacterial biofilm formation
title Wild mushroom extracts as inhibitors of bacterial biofilm formation
spellingShingle Wild mushroom extracts as inhibitors of bacterial biofilm formation
Alves, Maria José
Clinical isolates
Biofilm
Wild mushroom extracts
Multi-resistant
Cytotoxicity
title_short Wild mushroom extracts as inhibitors of bacterial biofilm formation
title_full Wild mushroom extracts as inhibitors of bacterial biofilm formation
title_fullStr Wild mushroom extracts as inhibitors of bacterial biofilm formation
title_full_unstemmed Wild mushroom extracts as inhibitors of bacterial biofilm formation
title_sort Wild mushroom extracts as inhibitors of bacterial biofilm formation
author Alves, Maria José
author_facet Alves, Maria José
Ferreira, Isabel C.F.R.
Lourenço, Inês
Costa, Eduardo Manuel
Martins, Anabela
Pintado, Manuela
author_role author
author2 Ferreira, Isabel C.F.R.
Lourenço, Inês
Costa, Eduardo Manuel
Martins, Anabela
Pintado, Manuela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Alves, Maria José
Ferreira, Isabel C.F.R.
Lourenço, Inês
Costa, Eduardo Manuel
Martins, Anabela
Pintado, Manuela
dc.subject.por.fl_str_mv Clinical isolates
Biofilm
Wild mushroom extracts
Multi-resistant
Cytotoxicity
topic Clinical isolates
Biofilm
Wild mushroom extracts
Multi-resistant
Cytotoxicity
description Microorganisms can colonize a wide variety of medical devices, putting patients in risk for local and systemic infectious complications, including local-site infections, catheter-related bloodstream infections, and endocarditis. These microorganisms are able to grow adhered to almost every surface, forming architecturally complex communities termed biofilms. The use of natural products has been extremely successful in the discovery of new medicine, and mushrooms could be a source of natural antimicrobials. The present study reports the capacity of wild mushroom extracts to inhibit in vitro biofilm formation by multi-resistant bacteria. Four Gram-negative bacteria biofilm producers (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, and Acinetobacter baumannii) isolated from urine were used to verify the activity of Russula delica, Fistulina hepatica, Mycena rosea, Leucopaxilus giganteus, and Lepista nuda extracts. The results obtained showed that all tested mushroom extracts presented some extent of inhibition of biofilm production. Pseudomonas aeruginosa was the microorganism with the highest capacity of biofilm production, being also the most susceptible to the extracts inhibition capacity (equal or higher than 50%). Among the five tested extracts against E. coli, Leucopaxillus giganteus (47.8%) and Mycenas rosea (44.8%) presented the highest inhibition of biofilm formation. The extracts exhibiting the highest inhibitory effect upon P. mirabilis biofilm formation were Sarcodon imbricatus (45.4%) and Russula delica (53.1%). Acinetobacter baumannii was the microorganism with the lowest susceptibility to mushroom extracts inhibitory effect on biofilm production (highest inhibition—almost 29%, by Russula delica extract). This is a pioneer study since, as far as we know, there are no reports on the inhibition of biofilm production by the studied mushroom extracts and in particular against multi-resistant clinical isolates; nevertheless, other studies are required to elucidate the mechanism of action.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2015-08-03T11:05:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/12027
url http://hdl.handle.net/10198/12027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, Maria José; Ferreira, Isabel C.F.R.; Lourenço, Inês; Costa, Eduardo; Martins, Anabela; Pintado, Manuela (2014). Wild mushroom extracts as inhibitors of bacterial biofilm formation. Pathogens. ISSN 2076-0817. 3:3, p. 667-679
2076-0817
10.3390/pathogens3030667
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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