Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane

Detalhes bibliográficos
Autor(a) principal: Duarte, Maria Salomé
Data de Publicação: 2018
Outros Autores: Silva, Sérgio A., Salvador, Andreia F., Cavaleiro, A. J., Stams, Alfons Johannes Maria, Alves, M. M., Pereira, M. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/55174
Resumo: Conversion of unsaturated long chain fatty acids (LCFA) to methane in continuous bioreactors is not fully understood. Palmitate (C16:0) often accumulates during oleate (C18:1) biodegradation in methanogenic bioreactors, and the reason why this happens and which microorganisms catalyze this reaction remains unknown. Facultative anaerobic bacteria are frequently found in continuous reactors operated at high LCFA loads, but their function is unclear. To get more insight on the role of these bacteria, LCFA conversion was studied under microaerophilic conditions. For that, we compared bioreactors treating oleate-based wastewater (organic loading rates of 1 and 3 kg COD m-3 d-1), operated under different redox conditions (strictly anaerobic-AnR, -350 mV; microaerophilic-MaR, -250 mV). At the higher load, palmitate accumulated 7 times more in the MaR, where facultative anaerobes were more abundant, and only the biomass from this reactor could recover the methanogenic activity after a transient inhibition. In a second experiment, the abundance of facultative anaerobic bacteria, particularly Pseudomonas spp. (from which two strains were isolated), was strongly correlated (p<0.05) with palmitate-to-total LCFA percentage in the biofilm formed in a continuous plug flow reactor fed with very high loads of oleate. This work strongly suggests that micro-aeration stimulates the development of facultative bacteria that are critical for achieving LCFA conversion to methane in continuous bioreactors. Microbial networks and interactions of facultative and strict anaerobes in microbial communities should be considered in future studies.
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spelling Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methaneScience & TechnologyConversion of unsaturated long chain fatty acids (LCFA) to methane in continuous bioreactors is not fully understood. Palmitate (C16:0) often accumulates during oleate (C18:1) biodegradation in methanogenic bioreactors, and the reason why this happens and which microorganisms catalyze this reaction remains unknown. Facultative anaerobic bacteria are frequently found in continuous reactors operated at high LCFA loads, but their function is unclear. To get more insight on the role of these bacteria, LCFA conversion was studied under microaerophilic conditions. For that, we compared bioreactors treating oleate-based wastewater (organic loading rates of 1 and 3 kg COD m-3 d-1), operated under different redox conditions (strictly anaerobic-AnR, -350 mV; microaerophilic-MaR, -250 mV). At the higher load, palmitate accumulated 7 times more in the MaR, where facultative anaerobes were more abundant, and only the biomass from this reactor could recover the methanogenic activity after a transient inhibition. In a second experiment, the abundance of facultative anaerobic bacteria, particularly Pseudomonas spp. (from which two strains were isolated), was strongly correlated (p<0.05) with palmitate-to-total LCFA percentage in the biofilm formed in a continuous plug flow reactor fed with very high loads of oleate. This work strongly suggests that micro-aeration stimulates the development of facultative bacteria that are critical for achieving LCFA conversion to methane in continuous bioreactors. Microbial networks and interactions of facultative and strict anaerobes in microbial communities should be considered in future studies.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER027462), the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 − Programa Operacional Regional do Norte. Research of M.S.D. and A.J.C. were supported by the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement No 323009.info:eu-repo/semantics/publishedVersionAmerican Chemical SocietyUniversidade do MinhoDuarte, Maria SaloméSilva, Sérgio A.Salvador, Andreia F.Cavaleiro, A. J.Stams, Alfons Johannes MariaAlves, M. M.Pereira, M. A.2018-05-152018-05-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/55174engDuarte, Maria Salomé; Silva, Sérgio A.; Salvador, Andreia F.; Cavaleiro, Ana Júlia; Stams, A. J. M.; Alves, M. Madalena; Pereira, M. Alcina, Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane. Environmental Science and Technology, 52(11), 6497-6507, 20180013-936X1520-585110.1021/acs.est.8b0089429763542http://pubs.acs.org/journal/esthaginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:46Zoai:repositorium.sdum.uminho.pt:1822/55174Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:35:17.543999Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane
title Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane
spellingShingle Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane
Duarte, Maria Salomé
Science & Technology
title_short Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane
title_full Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane
title_fullStr Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane
title_full_unstemmed Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane
title_sort Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane
author Duarte, Maria Salomé
author_facet Duarte, Maria Salomé
Silva, Sérgio A.
Salvador, Andreia F.
Cavaleiro, A. J.
Stams, Alfons Johannes Maria
Alves, M. M.
Pereira, M. A.
author_role author
author2 Silva, Sérgio A.
Salvador, Andreia F.
Cavaleiro, A. J.
Stams, Alfons Johannes Maria
Alves, M. M.
Pereira, M. A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Duarte, Maria Salomé
Silva, Sérgio A.
Salvador, Andreia F.
Cavaleiro, A. J.
Stams, Alfons Johannes Maria
Alves, M. M.
Pereira, M. A.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Conversion of unsaturated long chain fatty acids (LCFA) to methane in continuous bioreactors is not fully understood. Palmitate (C16:0) often accumulates during oleate (C18:1) biodegradation in methanogenic bioreactors, and the reason why this happens and which microorganisms catalyze this reaction remains unknown. Facultative anaerobic bacteria are frequently found in continuous reactors operated at high LCFA loads, but their function is unclear. To get more insight on the role of these bacteria, LCFA conversion was studied under microaerophilic conditions. For that, we compared bioreactors treating oleate-based wastewater (organic loading rates of 1 and 3 kg COD m-3 d-1), operated under different redox conditions (strictly anaerobic-AnR, -350 mV; microaerophilic-MaR, -250 mV). At the higher load, palmitate accumulated 7 times more in the MaR, where facultative anaerobes were more abundant, and only the biomass from this reactor could recover the methanogenic activity after a transient inhibition. In a second experiment, the abundance of facultative anaerobic bacteria, particularly Pseudomonas spp. (from which two strains were isolated), was strongly correlated (p<0.05) with palmitate-to-total LCFA percentage in the biofilm formed in a continuous plug flow reactor fed with very high loads of oleate. This work strongly suggests that micro-aeration stimulates the development of facultative bacteria that are critical for achieving LCFA conversion to methane in continuous bioreactors. Microbial networks and interactions of facultative and strict anaerobes in microbial communities should be considered in future studies.
publishDate 2018
dc.date.none.fl_str_mv 2018-05-15
2018-05-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/55174
url http://hdl.handle.net/1822/55174
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Duarte, Maria Salomé; Silva, Sérgio A.; Salvador, Andreia F.; Cavaleiro, Ana Júlia; Stams, A. J. M.; Alves, M. Madalena; Pereira, M. Alcina, Insight into the role of facultative bacteria stimulated by micro-aeration in continuous bioreactors converting LCFA to methane. Environmental Science and Technology, 52(11), 6497-6507, 2018
0013-936X
1520-5851
10.1021/acs.est.8b00894
29763542
http://pubs.acs.org/journal/esthag
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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