Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands

Detalhes bibliográficos
Autor(a) principal: Couto, J.
Data de Publicação: 2017
Outros Autores: Antunes, S., Pinheiro-Silva, R., Do Rosário, V., De La Fuente, J., Domingos, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1038/s41598-017-06317-6
Resumo: Malaria is caused by mosquito-borne Plasmodium spp. parasites that must infect and survive within mosquito salivary glands (SGs) prior to host transmission. Recent advances in transcriptomics and the complete genome sequencing of mosquito vectors have increased our knowledge of the SG genes and proteins involved in pathogen infection and transmission. Membrane solute carriers are key proteins involved in drug transport and are useful in the development of new interventions for transmission blocking. Herein, we applied transcriptomics analysis to compare SGs mRNA levels in Anopheles stephensi fed on non-infected and P. berghei-infected mice. The A. stephensi solute carriers prestinA and NDAE1 were up-regulated in response to infection. These molecules are predicted to interact with each other, and are reportedly involved in the maintenance of cell homeostasis. To further evaluate their functions in mosquito survival and parasite infection, these genes were knocked down by RNA interference. Knockdown of prestinA and NDAE1 resulted in reduction of the number of sporozoites in mosquito SGs. Moreover, NDAE1 knockdown strongly impacted mosquito survival, resulting in the death of half of the treated mosquitoes. Overall, our findings indicate the importance of prestinA and NDAE1 in interactions between mosquito SGs and Plasmodium, and suggest the need for further research.
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spelling Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glandsBiochemistry, Genetics and Molecular Biology (miscellaneous)ParasitologyInsect ScienceInfectious DiseasesSDG 3 - Good Health and Well-beingMalaria is caused by mosquito-borne Plasmodium spp. parasites that must infect and survive within mosquito salivary glands (SGs) prior to host transmission. Recent advances in transcriptomics and the complete genome sequencing of mosquito vectors have increased our knowledge of the SG genes and proteins involved in pathogen infection and transmission. Membrane solute carriers are key proteins involved in drug transport and are useful in the development of new interventions for transmission blocking. Herein, we applied transcriptomics analysis to compare SGs mRNA levels in Anopheles stephensi fed on non-infected and P. berghei-infected mice. The A. stephensi solute carriers prestinA and NDAE1 were up-regulated in response to infection. These molecules are predicted to interact with each other, and are reportedly involved in the maintenance of cell homeostasis. To further evaluate their functions in mosquito survival and parasite infection, these genes were knocked down by RNA interference. Knockdown of prestinA and NDAE1 resulted in reduction of the number of sporozoites in mosquito SGs. Moreover, NDAE1 knockdown strongly impacted mosquito survival, resulting in the death of half of the treated mosquitoes. Overall, our findings indicate the importance of prestinA and NDAE1 in interactions between mosquito SGs and Plasmodium, and suggest the need for further research.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNCouto, J.Antunes, S.Pinheiro-Silva, R.Do Rosário, V.De La Fuente, J.Domingos, A.2018-05-10T22:14:54Z2017-12-012017-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttps://doi.org/10.1038/s41598-017-06317-6eng2045-2322PURE: 3179969http://www.scopus.com/inward/record.url?scp=85025661813&partnerID=8YFLogxKhttps://doi.org/10.1038/s41598-017-06317-6info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:19:49Zoai:run.unl.pt:10362/36448Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:29.052709Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
title Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
spellingShingle Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
Couto, J.
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Parasitology
Insect Science
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
title_full Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
title_fullStr Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
title_full_unstemmed Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
title_sort Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands
author Couto, J.
author_facet Couto, J.
Antunes, S.
Pinheiro-Silva, R.
Do Rosário, V.
De La Fuente, J.
Domingos, A.
author_role author
author2 Antunes, S.
Pinheiro-Silva, R.
Do Rosário, V.
De La Fuente, J.
Domingos, A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv Couto, J.
Antunes, S.
Pinheiro-Silva, R.
Do Rosário, V.
De La Fuente, J.
Domingos, A.
dc.subject.por.fl_str_mv Biochemistry, Genetics and Molecular Biology (miscellaneous)
Parasitology
Insect Science
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Biochemistry, Genetics and Molecular Biology (miscellaneous)
Parasitology
Insect Science
Infectious Diseases
SDG 3 - Good Health and Well-being
description Malaria is caused by mosquito-borne Plasmodium spp. parasites that must infect and survive within mosquito salivary glands (SGs) prior to host transmission. Recent advances in transcriptomics and the complete genome sequencing of mosquito vectors have increased our knowledge of the SG genes and proteins involved in pathogen infection and transmission. Membrane solute carriers are key proteins involved in drug transport and are useful in the development of new interventions for transmission blocking. Herein, we applied transcriptomics analysis to compare SGs mRNA levels in Anopheles stephensi fed on non-infected and P. berghei-infected mice. The A. stephensi solute carriers prestinA and NDAE1 were up-regulated in response to infection. These molecules are predicted to interact with each other, and are reportedly involved in the maintenance of cell homeostasis. To further evaluate their functions in mosquito survival and parasite infection, these genes were knocked down by RNA interference. Knockdown of prestinA and NDAE1 resulted in reduction of the number of sporozoites in mosquito SGs. Moreover, NDAE1 knockdown strongly impacted mosquito survival, resulting in the death of half of the treated mosquitoes. Overall, our findings indicate the importance of prestinA and NDAE1 in interactions between mosquito SGs and Plasmodium, and suggest the need for further research.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
2017-12-01T00:00:00Z
2018-05-10T22:14:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1038/s41598-017-06317-6
url https://doi.org/10.1038/s41598-017-06317-6
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 3179969
http://www.scopus.com/inward/record.url?scp=85025661813&partnerID=8YFLogxK
https://doi.org/10.1038/s41598-017-06317-6
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