Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport

Detalhes bibliográficos
Autor(a) principal: Sousa, Maria João
Data de Publicação: 1992
Outros Autores: Mota, M., Leão, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/41784
Resumo: The transport system for malic acid present in Schizosaccharomyces pombe cells, growing in batch culture on several corbon sources, has been studied. It was found that the diarboxylic acid carrier of S. pombe is a proton-dicarboxylate symporter that allows transport and accumulation as a function of pH with the following kinetic parameters at pH 5·0: Vmax = 0·01 nmol of total malic acids 1 mg (dry weight) of cells, 1and Km = 0·1mM total malic acid uptake (pH 5·0) was accompanied by disappearance of extracellular protons, the uptake rates of which followed Michaelis-Menten kinetics as a function of the acid concentration. The Km values, calculated as the concentrations either of anions or of undissociated acid, at various extracellular pH values, pointed to the monoanionic form as the transported species. Furthermore, accumulated free acid suffered rapid efflux after the addition of the portonophore carbonyl cyanid m-chlorophenyl hydrazone. These results suggested that the transport system was a dicarboxylateproton symporter. Growth of cells in a medium with glucose (up to 14%, w/v) and malic acid (1·5%, w/v) also resulted in proton-dicarboxylate activity, suggesting that the system, besides being constitutive, was still active at high glucose concentrations. The following dicarboxylic acids acted as competitive inhibitors of malic acid transport at pH 5·0: D- malic acid, succinic acid, fumaric acid oxaloacetic acid, -Ketoglutaric acid, maleic acid, maleic and malonic acid. In addition all of these dicarboxylic acids induced proton movements that followed MichaelisMenten kinetics. It was concluded that the malic negatively charged form (probably the monoanionic form) was transported by a proton-symport mechanism and that the carrier appeared to be a common dicarboxylat transport sysmem. The undissociated acid entered the cells slowly by simple diffusion.
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spelling Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symportSchizosaccharomycesMalic acidTransportSaccharomycescerevisiaeScience & TechnologyThe transport system for malic acid present in Schizosaccharomyces pombe cells, growing in batch culture on several corbon sources, has been studied. It was found that the diarboxylic acid carrier of S. pombe is a proton-dicarboxylate symporter that allows transport and accumulation as a function of pH with the following kinetic parameters at pH 5·0: Vmax = 0·01 nmol of total malic acids 1 mg (dry weight) of cells, 1and Km = 0·1mM total malic acid uptake (pH 5·0) was accompanied by disappearance of extracellular protons, the uptake rates of which followed Michaelis-Menten kinetics as a function of the acid concentration. The Km values, calculated as the concentrations either of anions or of undissociated acid, at various extracellular pH values, pointed to the monoanionic form as the transported species. Furthermore, accumulated free acid suffered rapid efflux after the addition of the portonophore carbonyl cyanid m-chlorophenyl hydrazone. These results suggested that the transport system was a dicarboxylateproton symporter. Growth of cells in a medium with glucose (up to 14%, w/v) and malic acid (1·5%, w/v) also resulted in proton-dicarboxylate activity, suggesting that the system, besides being constitutive, was still active at high glucose concentrations. The following dicarboxylic acids acted as competitive inhibitors of malic acid transport at pH 5·0: D- malic acid, succinic acid, fumaric acid oxaloacetic acid, -Ketoglutaric acid, maleic acid, maleic and malonic acid. In addition all of these dicarboxylic acids induced proton movements that followed MichaelisMenten kinetics. It was concluded that the malic negatively charged form (probably the monoanionic form) was transported by a proton-symport mechanism and that the carrier appeared to be a common dicarboxylat transport sysmem. The undissociated acid entered the cells slowly by simple diffusion.(undefined)John Wiley and SonsUniversidade do MinhoSousa, Maria JoãoMota, M.Leão, Cecília19921992-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/41784engSousa, M.; Mota, M.; Leão, C., Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport. Yeast, 8(12), 1025-1031, 19920749-503X1097-006110.1002/yea.3200812051293882http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0061info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:49Zoai:repositorium.sdum.uminho.pt:1822/41784Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:30:35.017936Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport
title Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport
spellingShingle Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport
Sousa, Maria João
Schizosaccharomyces
Malic acid
Transport
Saccharomycescerevisiae
Science & Technology
title_short Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport
title_full Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport
title_fullStr Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport
title_full_unstemmed Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport
title_sort Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport
author Sousa, Maria João
author_facet Sousa, Maria João
Mota, M.
Leão, Cecília
author_role author
author2 Mota, M.
Leão, Cecília
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Sousa, Maria João
Mota, M.
Leão, Cecília
dc.subject.por.fl_str_mv Schizosaccharomyces
Malic acid
Transport
Saccharomycescerevisiae
Science & Technology
topic Schizosaccharomyces
Malic acid
Transport
Saccharomycescerevisiae
Science & Technology
description The transport system for malic acid present in Schizosaccharomyces pombe cells, growing in batch culture on several corbon sources, has been studied. It was found that the diarboxylic acid carrier of S. pombe is a proton-dicarboxylate symporter that allows transport and accumulation as a function of pH with the following kinetic parameters at pH 5·0: Vmax = 0·01 nmol of total malic acids 1 mg (dry weight) of cells, 1and Km = 0·1mM total malic acid uptake (pH 5·0) was accompanied by disappearance of extracellular protons, the uptake rates of which followed Michaelis-Menten kinetics as a function of the acid concentration. The Km values, calculated as the concentrations either of anions or of undissociated acid, at various extracellular pH values, pointed to the monoanionic form as the transported species. Furthermore, accumulated free acid suffered rapid efflux after the addition of the portonophore carbonyl cyanid m-chlorophenyl hydrazone. These results suggested that the transport system was a dicarboxylateproton symporter. Growth of cells in a medium with glucose (up to 14%, w/v) and malic acid (1·5%, w/v) also resulted in proton-dicarboxylate activity, suggesting that the system, besides being constitutive, was still active at high glucose concentrations. The following dicarboxylic acids acted as competitive inhibitors of malic acid transport at pH 5·0: D- malic acid, succinic acid, fumaric acid oxaloacetic acid, -Ketoglutaric acid, maleic acid, maleic and malonic acid. In addition all of these dicarboxylic acids induced proton movements that followed MichaelisMenten kinetics. It was concluded that the malic negatively charged form (probably the monoanionic form) was transported by a proton-symport mechanism and that the carrier appeared to be a common dicarboxylat transport sysmem. The undissociated acid entered the cells slowly by simple diffusion.
publishDate 1992
dc.date.none.fl_str_mv 1992
1992-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/41784
url http://hdl.handle.net/1822/41784
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sousa, M.; Mota, M.; Leão, C., Transport of malic acid in the yeast Schizosaccharomyces pombe: evidence for a proton-dicarboxylate symport. Yeast, 8(12), 1025-1031, 1992
0749-503X
1097-0061
10.1002/yea.320081205
1293882
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0061
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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