Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics

Detalhes bibliográficos
Autor(a) principal: Alves, Maria José
Data de Publicação: 2012
Outros Autores: Ferreira, Isabel C.F.R., Martins, Anabela, Pintado, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/7814
Resumo: Aims: This work aimed to screen the antimicrobial activity of aqueous methanolic extracts of 13 mushroom species, collected in Braganc¸a, against several clinical isolates obtained in Hospital Center of Tra´s-os-Montes and Alto Douro, Portugal. Methods and Results: Microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). MIC results showed that Russula delica and Fistulina hepatica extracts inhibited the growth of gram-negative (Escherichia coli, Morganella morganni and Pasteurella multocida) and gram-positive (Staphylococcus aureus, MRSA, Enterococcus faecalis, Listeria monocytogenes, Streptococcus agalactiae and Streptococcus pyogenes) bacteria. A bactericide effect of both extracts was observed in Past. multocida, Strep. agalactiae and Strep. pyogenes with MBC of 20, 10 and 5 mg ml 1, respectively. Lepista nuda extract exhibited a bactericide effect upon Past. multocida at 5 mg ml 1 and inhibited Proteus mirabilis at 20 mg ml 1. Ramaria botrytis extract showed activity against Enterococcus faecalis and L. monocytogenes, being bactericide for Past. multocida, Strep. agalactiae (MBCs 20 mg ml 1) and Strep. pyogenes (MBC 10 mg ml 1). Leucopaxillus giganteus extract inhibited the growth of E. coli and Pr. mirabilis, being bactericide for Past. multocida, Strep. pyogenes and Strep. agalactiae. Conclusions: Fistulina hepatica, R. botrytis and R. delica are the most promising species as antimicrobial agents. Significance and Impact of the Study: Mushroom extracts could be an alternative as antimicrobials against pathogenic micro-organisms resistant to conventional treatments.
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spelling Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibioticsAntimicrobial activityClinical isolatesNortheast PortugalWild mushroomsAims: This work aimed to screen the antimicrobial activity of aqueous methanolic extracts of 13 mushroom species, collected in Braganc¸a, against several clinical isolates obtained in Hospital Center of Tra´s-os-Montes and Alto Douro, Portugal. Methods and Results: Microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). MIC results showed that Russula delica and Fistulina hepatica extracts inhibited the growth of gram-negative (Escherichia coli, Morganella morganni and Pasteurella multocida) and gram-positive (Staphylococcus aureus, MRSA, Enterococcus faecalis, Listeria monocytogenes, Streptococcus agalactiae and Streptococcus pyogenes) bacteria. A bactericide effect of both extracts was observed in Past. multocida, Strep. agalactiae and Strep. pyogenes with MBC of 20, 10 and 5 mg ml 1, respectively. Lepista nuda extract exhibited a bactericide effect upon Past. multocida at 5 mg ml 1 and inhibited Proteus mirabilis at 20 mg ml 1. Ramaria botrytis extract showed activity against Enterococcus faecalis and L. monocytogenes, being bactericide for Past. multocida, Strep. agalactiae (MBCs 20 mg ml 1) and Strep. pyogenes (MBC 10 mg ml 1). Leucopaxillus giganteus extract inhibited the growth of E. coli and Pr. mirabilis, being bactericide for Past. multocida, Strep. pyogenes and Strep. agalactiae. Conclusions: Fistulina hepatica, R. botrytis and R. delica are the most promising species as antimicrobial agents. Significance and Impact of the Study: Mushroom extracts could be an alternative as antimicrobials against pathogenic micro-organisms resistant to conventional treatments.WileyBiblioteca Digital do IPBAlves, Maria JoséFerreira, Isabel C.F.R.Martins, AnabelaPintado, Manuela2013-01-04T12:30:22Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10198/7814engAlves, Maria José; Ferreira, Isabel C.F.R.; Martins, Anabela; Pintado, Manuela (2012). Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics. Journal of Apllied Microbiology. ISSN 1364-5072. 113:2, p. 466-4751364-507210.1111/j.1365-2672.2012.05347.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:18:13Zoai:bibliotecadigital.ipb.pt:10198/7814Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:59:07.963861Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics
title Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics
spellingShingle Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics
Alves, Maria José
Antimicrobial activity
Clinical isolates
Northeast Portugal
Wild mushrooms
title_short Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics
title_full Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics
title_fullStr Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics
title_full_unstemmed Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics
title_sort Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics
author Alves, Maria José
author_facet Alves, Maria José
Ferreira, Isabel C.F.R.
Martins, Anabela
Pintado, Manuela
author_role author
author2 Ferreira, Isabel C.F.R.
Martins, Anabela
Pintado, Manuela
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Alves, Maria José
Ferreira, Isabel C.F.R.
Martins, Anabela
Pintado, Manuela
dc.subject.por.fl_str_mv Antimicrobial activity
Clinical isolates
Northeast Portugal
Wild mushrooms
topic Antimicrobial activity
Clinical isolates
Northeast Portugal
Wild mushrooms
description Aims: This work aimed to screen the antimicrobial activity of aqueous methanolic extracts of 13 mushroom species, collected in Braganc¸a, against several clinical isolates obtained in Hospital Center of Tra´s-os-Montes and Alto Douro, Portugal. Methods and Results: Microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). MIC results showed that Russula delica and Fistulina hepatica extracts inhibited the growth of gram-negative (Escherichia coli, Morganella morganni and Pasteurella multocida) and gram-positive (Staphylococcus aureus, MRSA, Enterococcus faecalis, Listeria monocytogenes, Streptococcus agalactiae and Streptococcus pyogenes) bacteria. A bactericide effect of both extracts was observed in Past. multocida, Strep. agalactiae and Strep. pyogenes with MBC of 20, 10 and 5 mg ml 1, respectively. Lepista nuda extract exhibited a bactericide effect upon Past. multocida at 5 mg ml 1 and inhibited Proteus mirabilis at 20 mg ml 1. Ramaria botrytis extract showed activity against Enterococcus faecalis and L. monocytogenes, being bactericide for Past. multocida, Strep. agalactiae (MBCs 20 mg ml 1) and Strep. pyogenes (MBC 10 mg ml 1). Leucopaxillus giganteus extract inhibited the growth of E. coli and Pr. mirabilis, being bactericide for Past. multocida, Strep. pyogenes and Strep. agalactiae. Conclusions: Fistulina hepatica, R. botrytis and R. delica are the most promising species as antimicrobial agents. Significance and Impact of the Study: Mushroom extracts could be an alternative as antimicrobials against pathogenic micro-organisms resistant to conventional treatments.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2013-01-04T12:30:22Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/7814
url http://hdl.handle.net/10198/7814
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, Maria José; Ferreira, Isabel C.F.R.; Martins, Anabela; Pintado, Manuela (2012). Antimicrobial activity of wild mushrooms extracts against clinical isolates resistant to different antibiotics. Journal of Apllied Microbiology. ISSN 1364-5072. 113:2, p. 466-475
1364-5072
10.1111/j.1365-2672.2012.05347.x
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