Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L.
Main Author: | |
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Publication Date: | 2012 |
Other Authors: | , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10174/5290 |
Summary: | Stone pine (Pinus pinea L.) is an economically important forest tree in the Mediterranean region and has been the target of breeding and selection through micropropagation mainly for its ecological and ornamental aspects. A crucial step in micropropagation is adventitious rooting of microshoots, which often is highly inefficient in most conifer species including stone pine. Hence, we conducted in vitro co-culture of Pinus pinea microshoots with the ectomycorrhizal fungus Pisolithus arhizus (isolated from natural stands) in order to promote adventitious root growth and plant survival during acclimatization. Significant differences were found in the number of branches, in the number of roots plus branches, in total length of roots, in total length of roots plus branches, in average root length and in the length of the longest root in inoculated plants during in vitro rooting compared with non-inoculated plants. The roots of inoculated plants also grew better in vermiculite and during acclimatization in a mixed substrate compared with roots of control plants resulting in the development of vigorous root system. Overall, mycorrhizal inoculation increased the survival rate of the regenerated pine. |
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Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L.Stone pinemycorrhizationco-culturebiotizationacclimationStone pine (Pinus pinea L.) is an economically important forest tree in the Mediterranean region and has been the target of breeding and selection through micropropagation mainly for its ecological and ornamental aspects. A crucial step in micropropagation is adventitious rooting of microshoots, which often is highly inefficient in most conifer species including stone pine. Hence, we conducted in vitro co-culture of Pinus pinea microshoots with the ectomycorrhizal fungus Pisolithus arhizus (isolated from natural stands) in order to promote adventitious root growth and plant survival during acclimatization. Significant differences were found in the number of branches, in the number of roots plus branches, in total length of roots, in total length of roots plus branches, in average root length and in the length of the longest root in inoculated plants during in vitro rooting compared with non-inoculated plants. The roots of inoculated plants also grew better in vermiculite and during acclimatization in a mixed substrate compared with roots of control plants resulting in the development of vigorous root system. Overall, mycorrhizal inoculation increased the survival rate of the regenerated pine.Propagation of Ornamental Plants2012-09-24T17:16:16Z2012-09-242012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/5290http://hdl.handle.net/10174/5290engRagonezi C, Caldeira AT, Martins MR, Dias LS, Santos-Silva C, Ganhão E, Miralto O, Pereira I, Louro R, Klimaszewska K, Zavattieri A 2012. Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. Propagation of Ornamental Plants 12: 139-147cazi04@yahoo.com.bratc@uevora.ptmrm@uevora.ptndcss@uevora.ptemng@uevora.ptmos@uevora.ptipp@uevora.ptndndzavattieri@uevora.pt578Ragonezi, C.Caldeira, A.T.Martins, M.R.Dias, L.S.Santos-Silva, C.Ganhão, E.Miralto, O.Pereira, I.Louro, R.Klimaszewska, K.Zavattieri, A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:43:49Zoai:dspace.uevora.pt:10174/5290Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:00:17.520387Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. |
title |
Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. |
spellingShingle |
Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. Ragonezi, C. Stone pine mycorrhization co-culture biotization acclimation |
title_short |
Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. |
title_full |
Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. |
title_fullStr |
Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. |
title_full_unstemmed |
Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. |
title_sort |
Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. |
author |
Ragonezi, C. |
author_facet |
Ragonezi, C. Caldeira, A.T. Martins, M.R. Dias, L.S. Santos-Silva, C. Ganhão, E. Miralto, O. Pereira, I. Louro, R. Klimaszewska, K. Zavattieri, A. |
author_role |
author |
author2 |
Caldeira, A.T. Martins, M.R. Dias, L.S. Santos-Silva, C. Ganhão, E. Miralto, O. Pereira, I. Louro, R. Klimaszewska, K. Zavattieri, A. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ragonezi, C. Caldeira, A.T. Martins, M.R. Dias, L.S. Santos-Silva, C. Ganhão, E. Miralto, O. Pereira, I. Louro, R. Klimaszewska, K. Zavattieri, A. |
dc.subject.por.fl_str_mv |
Stone pine mycorrhization co-culture biotization acclimation |
topic |
Stone pine mycorrhization co-culture biotization acclimation |
description |
Stone pine (Pinus pinea L.) is an economically important forest tree in the Mediterranean region and has been the target of breeding and selection through micropropagation mainly for its ecological and ornamental aspects. A crucial step in micropropagation is adventitious rooting of microshoots, which often is highly inefficient in most conifer species including stone pine. Hence, we conducted in vitro co-culture of Pinus pinea microshoots with the ectomycorrhizal fungus Pisolithus arhizus (isolated from natural stands) in order to promote adventitious root growth and plant survival during acclimatization. Significant differences were found in the number of branches, in the number of roots plus branches, in total length of roots, in total length of roots plus branches, in average root length and in the length of the longest root in inoculated plants during in vitro rooting compared with non-inoculated plants. The roots of inoculated plants also grew better in vermiculite and during acclimatization in a mixed substrate compared with roots of control plants resulting in the development of vigorous root system. Overall, mycorrhizal inoculation increased the survival rate of the regenerated pine. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-24T17:16:16Z 2012-09-24 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/5290 http://hdl.handle.net/10174/5290 |
url |
http://hdl.handle.net/10174/5290 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ragonezi C, Caldeira AT, Martins MR, Dias LS, Santos-Silva C, Ganhão E, Miralto O, Pereira I, Louro R, Klimaszewska K, Zavattieri A 2012. Pisolithus arhizus (Scop.) Rauschert improves growth of adventitious roots and acclimatization of in vitro regenerated plantlets of Pinus pinea L. Propagation of Ornamental Plants 12: 139-147 cazi04@yahoo.com.br atc@uevora.pt mrm@uevora.pt nd css@uevora.pt emng@uevora.pt mos@uevora.pt ipp@uevora.pt nd nd zavattieri@uevora.pt 578 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Propagation of Ornamental Plants |
publisher.none.fl_str_mv |
Propagation of Ornamental Plants |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136485332484096 |