Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells
Main Author: | |
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Publication Date: | 2018 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/56324 |
Summary: | Gravity influences physical and biological processes, especially during development and homeostasis of several tissues in the human body. Studies under altered gravity have been receiving great attention towards a better understanding of microgravity, hypogravity (<1g) or hypergravity (>1g) induced alterations. In the present work, the influence of simulated hypergravity over human tendon-derived cells (hTDCs) was studied at 5, 10, 15 and 20g for 4 or 16 h, using a large diameter centrifuge (LDC). Main results showed that 16 h of simulated hypergravity limited cell proliferation. Cell area was higher in hTDCs cultured at 5, 10 and 15g for 16 h, in comparison to 1g control. Actin filaments were more pronounced in hTDCs cultured at 5 and 10g for 16 h. Focal adhesion kinase (FAK) was mainly expressed in focal adhesion sites upon hypergravity stimulation, in comparison to perinuclear localization in control cells after 16 h; and FAK number/cell increased with increasing g-levels. A tendency towards an up-regulation of tenogenic markers was observed; SCX, TNC, COL3A1 and DCN were significantly up-regulated in hTDCs cultured at 15g and COL3A1 and DCN were significantly up-regulated in hTDCs cultured at 20g. Overall, simulated hypergravity affected the behavior of hTDCs, with more pronounced effects in the long-term period (16 h) of stimulation. |
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Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cellsaltered gravityCytoskeletonF-actinlarge diameter centrifugeMechanosensingTenogenic markersScience & TechnologyGravity influences physical and biological processes, especially during development and homeostasis of several tissues in the human body. Studies under altered gravity have been receiving great attention towards a better understanding of microgravity, hypogravity (<1g) or hypergravity (>1g) induced alterations. In the present work, the influence of simulated hypergravity over human tendon-derived cells (hTDCs) was studied at 5, 10, 15 and 20g for 4 or 16 h, using a large diameter centrifuge (LDC). Main results showed that 16 h of simulated hypergravity limited cell proliferation. Cell area was higher in hTDCs cultured at 5, 10 and 15g for 16 h, in comparison to 1g control. Actin filaments were more pronounced in hTDCs cultured at 5 and 10g for 16 h. Focal adhesion kinase (FAK) was mainly expressed in focal adhesion sites upon hypergravity stimulation, in comparison to perinuclear localization in control cells after 16 h; and FAK number/cell increased with increasing g-levels. A tendency towards an up-regulation of tenogenic markers was observed; SCX, TNC, COL3A1 and DCN were significantly up-regulated in hTDCs cultured at 15g and COL3A1 and DCN were significantly up-regulated in hTDCs cultured at 20g. Overall, simulated hypergravity affected the behavior of hTDCs, with more pronounced effects in the long-term period (16 h) of stimulation.The experiments reported here were performed in the framework of the Spin Your Thesis! 2016 programme, organized by ESA Education Office. Authors are grateful to the staff of ESA Education Office, Nigel Savage and Lukas Pfeiffer, for the insightful discussion and support; and to Alan Dowson and Elsa Silva for the technical support before and during the campaign. The authors would like to thank to Hospital da Prelada (Porto, Portugal) for providing tendon tissue samples. Authors also acknowledge Portuguese funds through FCT – Fundação para a Ciência e a Tecnologia in the framework of FCT‐POPH‐FSE, the PhD grant SFRH/BD/96593/2013 of R.C‐A and the consolidator grant IF/00593/2015 of M.E.G.; to the project RL3‐TECT‐NORTE‐07‐0124‐FEDER‐000020 cofinanced by ON.2 under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF). info:eu-repo/semantics/publishedVersionMary Ann Liebert Inc.Universidade do MinhoCosta-Almeida, R.Carvalho, D. T. O.Ferreira, M. J. S.Pesqueira, T.Monici, M.van Loon, J. J. W. A.Granja, P. L.Gomes, M. E.2018-032018-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/56324engCosta-Almeida R., Carvalho D. T. O., Ferreira M. J. S., Pesqueira T., Monici M., van Loon J. J. W. A., Granja P. L., Gomes M. E. Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells, Stem Cells And Development, Vol. 27, Issue 12, pp. 858-869, doi:10.1089/scd.2017.0206, 20181547-328710.1089/scd.2017.020629649412https://www.liebertpub.com/doi/10.1089/scd.2017.0206info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:26Zoai:repositorium.sdum.uminho.pt:1822/56324Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:10:04.460316Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells |
title |
Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells |
spellingShingle |
Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells Costa-Almeida, R. altered gravity Cytoskeleton F-actin large diameter centrifuge Mechanosensing Tenogenic markers Science & Technology |
title_short |
Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells |
title_full |
Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells |
title_fullStr |
Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells |
title_full_unstemmed |
Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells |
title_sort |
Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells |
author |
Costa-Almeida, R. |
author_facet |
Costa-Almeida, R. Carvalho, D. T. O. Ferreira, M. J. S. Pesqueira, T. Monici, M. van Loon, J. J. W. A. Granja, P. L. Gomes, M. E. |
author_role |
author |
author2 |
Carvalho, D. T. O. Ferreira, M. J. S. Pesqueira, T. Monici, M. van Loon, J. J. W. A. Granja, P. L. Gomes, M. E. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Costa-Almeida, R. Carvalho, D. T. O. Ferreira, M. J. S. Pesqueira, T. Monici, M. van Loon, J. J. W. A. Granja, P. L. Gomes, M. E. |
dc.subject.por.fl_str_mv |
altered gravity Cytoskeleton F-actin large diameter centrifuge Mechanosensing Tenogenic markers Science & Technology |
topic |
altered gravity Cytoskeleton F-actin large diameter centrifuge Mechanosensing Tenogenic markers Science & Technology |
description |
Gravity influences physical and biological processes, especially during development and homeostasis of several tissues in the human body. Studies under altered gravity have been receiving great attention towards a better understanding of microgravity, hypogravity (<1g) or hypergravity (>1g) induced alterations. In the present work, the influence of simulated hypergravity over human tendon-derived cells (hTDCs) was studied at 5, 10, 15 and 20g for 4 or 16 h, using a large diameter centrifuge (LDC). Main results showed that 16 h of simulated hypergravity limited cell proliferation. Cell area was higher in hTDCs cultured at 5, 10 and 15g for 16 h, in comparison to 1g control. Actin filaments were more pronounced in hTDCs cultured at 5 and 10g for 16 h. Focal adhesion kinase (FAK) was mainly expressed in focal adhesion sites upon hypergravity stimulation, in comparison to perinuclear localization in control cells after 16 h; and FAK number/cell increased with increasing g-levels. A tendency towards an up-regulation of tenogenic markers was observed; SCX, TNC, COL3A1 and DCN were significantly up-regulated in hTDCs cultured at 15g and COL3A1 and DCN were significantly up-regulated in hTDCs cultured at 20g. Overall, simulated hypergravity affected the behavior of hTDCs, with more pronounced effects in the long-term period (16 h) of stimulation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03 2018-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/56324 |
url |
http://hdl.handle.net/1822/56324 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Costa-Almeida R., Carvalho D. T. O., Ferreira M. J. S., Pesqueira T., Monici M., van Loon J. J. W. A., Granja P. L., Gomes M. E. Continuous exposure to simulated hypergravity induced changes in proliferation, morphology and gene expression of human tendon cells, Stem Cells And Development, Vol. 27, Issue 12, pp. 858-869, doi:10.1089/scd.2017.0206, 2018 1547-3287 10.1089/scd.2017.0206 29649412 https://www.liebertpub.com/doi/10.1089/scd.2017.0206 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert Inc. |
publisher.none.fl_str_mv |
Mary Ann Liebert Inc. |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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