Development of an electrochemical biosensor for Galectin-3 detection in point-of-care

Detalhes bibliográficos
Autor(a) principal: Cerqueira, Sofia M.V.
Data de Publicação: 2021
Outros Autores: Fernandes, Rúben, Moreira, Felismina, Sales, Maria Goreti Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/18563
Resumo: This research work aims the development and optimization of an electrochemical biosensor based on molecularly-imprinted polymers [MIPs], for monitoring a melanoma biomarker, Galectin-3 (Gal-3). As it is a multifunctional protein that plays an important role in different types of tumors including melanoma, and has shown good results as a potential biomarker in several areas, the construction of a biosensor for the detection of this protein would be a simple and quick strategy to support the treatment of this type of pathology. The target molecule was recognized by a MIP material, created on the electrode’s surface by electropolymerizing a mixture of analyte (Gal-3) and monomer (2-aminophenol). Then, the protein was removed from the polymeric material by oxalic acid treatment. This process formed a non-conductive polymer with recognition sites showing affinity for the Gal-3. The control of the surface modification was monitored by Raman spectroscopy and electroanalytical techniques, namely electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The analytical performance of the sensor was evaluated by EIS, by following the analytical response of standard solutions ranging from 0.5 ng/mL to 5000 ng/mL Gal-3 in spyked serum. In general, the biosensor displayed good analytical features, considering limit of detection, response time and reproducibility. Overall, this study resulted from the need to create a new strategy for monitoring melanoma through the creation of a cheaper, faster and sensitive device, which can be commercialized and thus integrate the entire process associated with the treatment and follow-up of this pathology.
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spelling Development of an electrochemical biosensor for Galectin-3 detection in point-of-careMelanomaScreen-printed-electrodeMolecularly-imprinted polymerBiosensorGalectin-3This research work aims the development and optimization of an electrochemical biosensor based on molecularly-imprinted polymers [MIPs], for monitoring a melanoma biomarker, Galectin-3 (Gal-3). As it is a multifunctional protein that plays an important role in different types of tumors including melanoma, and has shown good results as a potential biomarker in several areas, the construction of a biosensor for the detection of this protein would be a simple and quick strategy to support the treatment of this type of pathology. The target molecule was recognized by a MIP material, created on the electrode’s surface by electropolymerizing a mixture of analyte (Gal-3) and monomer (2-aminophenol). Then, the protein was removed from the polymeric material by oxalic acid treatment. This process formed a non-conductive polymer with recognition sites showing affinity for the Gal-3. The control of the surface modification was monitored by Raman spectroscopy and electroanalytical techniques, namely electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The analytical performance of the sensor was evaluated by EIS, by following the analytical response of standard solutions ranging from 0.5 ng/mL to 5000 ng/mL Gal-3 in spyked serum. In general, the biosensor displayed good analytical features, considering limit of detection, response time and reproducibility. Overall, this study resulted from the need to create a new strategy for monitoring melanoma through the creation of a cheaper, faster and sensitive device, which can be commercialized and thus integrate the entire process associated with the treatment and follow-up of this pathology.MindGAP –, Bridging the gap between Mind, Brain and Body: exosome role and monitoring, FET-Open/H2020, European Commission (Brussels), Grant agreement 829040. 0624_2IQBIONEURO_6_E, , Impulso de una red de I + i en química biológica para diagnóstico y tratamiento de enfermedades neurológicas EP – INTERREG V A España Portugal (POCTEP).ElsevierRepositório Científico do Instituto Politécnico do PortoCerqueira, Sofia M.V.Fernandes, RúbenMoreira, FelisminaSales, Maria Goreti Ferreira20212031-01-01T00:00:00Z2021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.22/18563eng10.1016/j.microc.2021.105992info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:09:54Zoai:recipp.ipp.pt:10400.22/18563Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:37:54.808819Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of an electrochemical biosensor for Galectin-3 detection in point-of-care
title Development of an electrochemical biosensor for Galectin-3 detection in point-of-care
spellingShingle Development of an electrochemical biosensor for Galectin-3 detection in point-of-care
Cerqueira, Sofia M.V.
Melanoma
Screen-printed-electrode
Molecularly-imprinted polymer
Biosensor
Galectin-3
title_short Development of an electrochemical biosensor for Galectin-3 detection in point-of-care
title_full Development of an electrochemical biosensor for Galectin-3 detection in point-of-care
title_fullStr Development of an electrochemical biosensor for Galectin-3 detection in point-of-care
title_full_unstemmed Development of an electrochemical biosensor for Galectin-3 detection in point-of-care
title_sort Development of an electrochemical biosensor for Galectin-3 detection in point-of-care
author Cerqueira, Sofia M.V.
author_facet Cerqueira, Sofia M.V.
Fernandes, Rúben
Moreira, Felismina
Sales, Maria Goreti Ferreira
author_role author
author2 Fernandes, Rúben
Moreira, Felismina
Sales, Maria Goreti Ferreira
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Cerqueira, Sofia M.V.
Fernandes, Rúben
Moreira, Felismina
Sales, Maria Goreti Ferreira
dc.subject.por.fl_str_mv Melanoma
Screen-printed-electrode
Molecularly-imprinted polymer
Biosensor
Galectin-3
topic Melanoma
Screen-printed-electrode
Molecularly-imprinted polymer
Biosensor
Galectin-3
description This research work aims the development and optimization of an electrochemical biosensor based on molecularly-imprinted polymers [MIPs], for monitoring a melanoma biomarker, Galectin-3 (Gal-3). As it is a multifunctional protein that plays an important role in different types of tumors including melanoma, and has shown good results as a potential biomarker in several areas, the construction of a biosensor for the detection of this protein would be a simple and quick strategy to support the treatment of this type of pathology. The target molecule was recognized by a MIP material, created on the electrode’s surface by electropolymerizing a mixture of analyte (Gal-3) and monomer (2-aminophenol). Then, the protein was removed from the polymeric material by oxalic acid treatment. This process formed a non-conductive polymer with recognition sites showing affinity for the Gal-3. The control of the surface modification was monitored by Raman spectroscopy and electroanalytical techniques, namely electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The analytical performance of the sensor was evaluated by EIS, by following the analytical response of standard solutions ranging from 0.5 ng/mL to 5000 ng/mL Gal-3 in spyked serum. In general, the biosensor displayed good analytical features, considering limit of detection, response time and reproducibility. Overall, this study resulted from the need to create a new strategy for monitoring melanoma through the creation of a cheaper, faster and sensitive device, which can be commercialized and thus integrate the entire process associated with the treatment and follow-up of this pathology.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2031-01-01T00:00:00Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/18563
url http://hdl.handle.net/10400.22/18563
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.microc.2021.105992
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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