Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
Main Author: | |
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Publication Date: | 2018 |
Other Authors: | , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | https://hdl.handle.net/10216/120511 |
Summary: | Marine sponges and cyanobacteria have a long history of co-evolution, with documented genome adaptations in cyanobionts. Both organisms are known to produce a wide variety of natural compounds, with only scarce information about novel natural compounds produced by cyanobionts. In the present study, we aimed to address their toxicological potential, isolating cyanobacteria (n = 12) from different sponge species from the coast of Portugal (mainland, Azores, and Madeira Islands). After large-scale growth, we obtained both organic and aqueous extracts to perform a series of ecologically-relevant bioassays. In the acute toxicity assay, using nauplii of Artemia salina, only organic extracts showed lethality, especially in picocyanobacterial strains. In the bioassay with Paracentrotus lividus, both organic and aqueous extracts produced embryogenic toxicity (respectively 58% and 36%), pointing to the presence of compounds that interfere with growth factors on cells. No development of pluteus larvae was observed for the organic extract of the strain Chroococcales 6MA13ti, indicating the presence of compounds that affect skeleton formation. In the hemolytic assay, none of the extracts induced red blood cells lysis. Organic extracts, especially from picoplanktonic strains, proved to be the most promising for future bioassay-guided fractionation and compounds isolation. This approach allows us to classify the compounds extracted from the cyanobacteria into effect categories and bioactivity profiles. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
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Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceansanimal tissueArtemia salinaArticlebacterium isolationbiomass productionChroococcalescontrolled studycyanobacteriumecotoxicologyhemoglobin blood levelhemolysis assaylethalitymarine environmentmicrobial diversitymousenonhumanParacentrotus lividusPortugalsponge (Porifera)SynechococcusSynechocystistoxicity assayMarine sponges and cyanobacteria have a long history of co-evolution, with documented genome adaptations in cyanobionts. Both organisms are known to produce a wide variety of natural compounds, with only scarce information about novel natural compounds produced by cyanobionts. In the present study, we aimed to address their toxicological potential, isolating cyanobacteria (n = 12) from different sponge species from the coast of Portugal (mainland, Azores, and Madeira Islands). After large-scale growth, we obtained both organic and aqueous extracts to perform a series of ecologically-relevant bioassays. In the acute toxicity assay, using nauplii of Artemia salina, only organic extracts showed lethality, especially in picocyanobacterial strains. In the bioassay with Paracentrotus lividus, both organic and aqueous extracts produced embryogenic toxicity (respectively 58% and 36%), pointing to the presence of compounds that interfere with growth factors on cells. No development of pluteus larvae was observed for the organic extract of the strain Chroococcales 6MA13ti, indicating the presence of compounds that affect skeleton formation. In the hemolytic assay, none of the extracts induced red blood cells lysis. Organic extracts, especially from picoplanktonic strains, proved to be the most promising for future bioassay-guided fractionation and compounds isolation. This approach allows us to classify the compounds extracted from the cyanobacteria into effect categories and bioactivity profiles. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.MDPI20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120511eng2072665110.3390/toxins10070297Regueiras A.Pereira S.Costa M.S.Vasconcelos V.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:40:30Zoai:repositorio-aberto.up.pt:10216/120511Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:45:23.921704Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans |
title |
Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans |
spellingShingle |
Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans Regueiras A. animal tissue Artemia salina Article bacterium isolation biomass production Chroococcales controlled study cyanobacterium ecotoxicology hemoglobin blood level hemolysis assay lethality marine environment microbial diversity mouse nonhuman Paracentrotus lividus Portugal sponge (Porifera) Synechococcus Synechocystis toxicity assay |
title_short |
Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans |
title_full |
Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans |
title_fullStr |
Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans |
title_full_unstemmed |
Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans |
title_sort |
Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans |
author |
Regueiras A. |
author_facet |
Regueiras A. Pereira S. Costa M.S. Vasconcelos V. |
author_role |
author |
author2 |
Pereira S. Costa M.S. Vasconcelos V. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Regueiras A. Pereira S. Costa M.S. Vasconcelos V. |
dc.subject.por.fl_str_mv |
animal tissue Artemia salina Article bacterium isolation biomass production Chroococcales controlled study cyanobacterium ecotoxicology hemoglobin blood level hemolysis assay lethality marine environment microbial diversity mouse nonhuman Paracentrotus lividus Portugal sponge (Porifera) Synechococcus Synechocystis toxicity assay |
topic |
animal tissue Artemia salina Article bacterium isolation biomass production Chroococcales controlled study cyanobacterium ecotoxicology hemoglobin blood level hemolysis assay lethality marine environment microbial diversity mouse nonhuman Paracentrotus lividus Portugal sponge (Porifera) Synechococcus Synechocystis toxicity assay |
description |
Marine sponges and cyanobacteria have a long history of co-evolution, with documented genome adaptations in cyanobionts. Both organisms are known to produce a wide variety of natural compounds, with only scarce information about novel natural compounds produced by cyanobionts. In the present study, we aimed to address their toxicological potential, isolating cyanobacteria (n = 12) from different sponge species from the coast of Portugal (mainland, Azores, and Madeira Islands). After large-scale growth, we obtained both organic and aqueous extracts to perform a series of ecologically-relevant bioassays. In the acute toxicity assay, using nauplii of Artemia salina, only organic extracts showed lethality, especially in picocyanobacterial strains. In the bioassay with Paracentrotus lividus, both organic and aqueous extracts produced embryogenic toxicity (respectively 58% and 36%), pointing to the presence of compounds that interfere with growth factors on cells. No development of pluteus larvae was observed for the organic extract of the strain Chroococcales 6MA13ti, indicating the presence of compounds that affect skeleton formation. In the hemolytic assay, none of the extracts induced red blood cells lysis. Organic extracts, especially from picoplanktonic strains, proved to be the most promising for future bioassay-guided fractionation and compounds isolation. This approach allows us to classify the compounds extracted from the cyanobacteria into effect categories and bioactivity profiles. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/120511 |
url |
https://hdl.handle.net/10216/120511 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
20726651 10.3390/toxins10070297 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135770557022208 |