Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans

Detalhes bibliográficos
Autor(a) principal: Regueiras A.
Data de Publicação: 2018
Outros Autores: Pereira S., Costa M.S., Vasconcelos V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/120511
Resumo: Marine sponges and cyanobacteria have a long history of co-evolution, with documented genome adaptations in cyanobionts. Both organisms are known to produce a wide variety of natural compounds, with only scarce information about novel natural compounds produced by cyanobionts. In the present study, we aimed to address their toxicological potential, isolating cyanobacteria (n = 12) from different sponge species from the coast of Portugal (mainland, Azores, and Madeira Islands). After large-scale growth, we obtained both organic and aqueous extracts to perform a series of ecologically-relevant bioassays. In the acute toxicity assay, using nauplii of Artemia salina, only organic extracts showed lethality, especially in picocyanobacterial strains. In the bioassay with Paracentrotus lividus, both organic and aqueous extracts produced embryogenic toxicity (respectively 58% and 36%), pointing to the presence of compounds that interfere with growth factors on cells. No development of pluteus larvae was observed for the organic extract of the strain Chroococcales 6MA13ti, indicating the presence of compounds that affect skeleton formation. In the hemolytic assay, none of the extracts induced red blood cells lysis. Organic extracts, especially from picoplanktonic strains, proved to be the most promising for future bioassay-guided fractionation and compounds isolation. This approach allows us to classify the compounds extracted from the cyanobacteria into effect categories and bioactivity profiles. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceansanimal tissueArtemia salinaArticlebacterium isolationbiomass productionChroococcalescontrolled studycyanobacteriumecotoxicologyhemoglobin blood levelhemolysis assaylethalitymarine environmentmicrobial diversitymousenonhumanParacentrotus lividusPortugalsponge (Porifera)SynechococcusSynechocystistoxicity assayMarine sponges and cyanobacteria have a long history of co-evolution, with documented genome adaptations in cyanobionts. Both organisms are known to produce a wide variety of natural compounds, with only scarce information about novel natural compounds produced by cyanobionts. In the present study, we aimed to address their toxicological potential, isolating cyanobacteria (n = 12) from different sponge species from the coast of Portugal (mainland, Azores, and Madeira Islands). After large-scale growth, we obtained both organic and aqueous extracts to perform a series of ecologically-relevant bioassays. In the acute toxicity assay, using nauplii of Artemia salina, only organic extracts showed lethality, especially in picocyanobacterial strains. In the bioassay with Paracentrotus lividus, both organic and aqueous extracts produced embryogenic toxicity (respectively 58% and 36%), pointing to the presence of compounds that interfere with growth factors on cells. No development of pluteus larvae was observed for the organic extract of the strain Chroococcales 6MA13ti, indicating the presence of compounds that affect skeleton formation. In the hemolytic assay, none of the extracts induced red blood cells lysis. Organic extracts, especially from picoplanktonic strains, proved to be the most promising for future bioassay-guided fractionation and compounds isolation. This approach allows us to classify the compounds extracted from the cyanobacteria into effect categories and bioactivity profiles. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.MDPI20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120511eng2072665110.3390/toxins10070297Regueiras A.Pereira S.Costa M.S.Vasconcelos V.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:40:30Zoai:repositorio-aberto.up.pt:10216/120511Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:45:23.921704Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
title Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
spellingShingle Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
Regueiras A.
animal tissue
Artemia salina
Article
bacterium isolation
biomass production
Chroococcales
controlled study
cyanobacterium
ecotoxicology
hemoglobin blood level
hemolysis assay
lethality
marine environment
microbial diversity
mouse
nonhuman
Paracentrotus lividus
Portugal
sponge (Porifera)
Synechococcus
Synechocystis
toxicity assay
title_short Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
title_full Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
title_fullStr Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
title_full_unstemmed Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
title_sort Differential toxicity of cyanobacteria isolated from marine sponges towards echinoderms and crustaceans
author Regueiras A.
author_facet Regueiras A.
Pereira S.
Costa M.S.
Vasconcelos V.
author_role author
author2 Pereira S.
Costa M.S.
Vasconcelos V.
author2_role author
author
author
dc.contributor.author.fl_str_mv Regueiras A.
Pereira S.
Costa M.S.
Vasconcelos V.
dc.subject.por.fl_str_mv animal tissue
Artemia salina
Article
bacterium isolation
biomass production
Chroococcales
controlled study
cyanobacterium
ecotoxicology
hemoglobin blood level
hemolysis assay
lethality
marine environment
microbial diversity
mouse
nonhuman
Paracentrotus lividus
Portugal
sponge (Porifera)
Synechococcus
Synechocystis
toxicity assay
topic animal tissue
Artemia salina
Article
bacterium isolation
biomass production
Chroococcales
controlled study
cyanobacterium
ecotoxicology
hemoglobin blood level
hemolysis assay
lethality
marine environment
microbial diversity
mouse
nonhuman
Paracentrotus lividus
Portugal
sponge (Porifera)
Synechococcus
Synechocystis
toxicity assay
description Marine sponges and cyanobacteria have a long history of co-evolution, with documented genome adaptations in cyanobionts. Both organisms are known to produce a wide variety of natural compounds, with only scarce information about novel natural compounds produced by cyanobionts. In the present study, we aimed to address their toxicological potential, isolating cyanobacteria (n = 12) from different sponge species from the coast of Portugal (mainland, Azores, and Madeira Islands). After large-scale growth, we obtained both organic and aqueous extracts to perform a series of ecologically-relevant bioassays. In the acute toxicity assay, using nauplii of Artemia salina, only organic extracts showed lethality, especially in picocyanobacterial strains. In the bioassay with Paracentrotus lividus, both organic and aqueous extracts produced embryogenic toxicity (respectively 58% and 36%), pointing to the presence of compounds that interfere with growth factors on cells. No development of pluteus larvae was observed for the organic extract of the strain Chroococcales 6MA13ti, indicating the presence of compounds that affect skeleton formation. In the hemolytic assay, none of the extracts induced red blood cells lysis. Organic extracts, especially from picoplanktonic strains, proved to be the most promising for future bioassay-guided fractionation and compounds isolation. This approach allows us to classify the compounds extracted from the cyanobacteria into effect categories and bioactivity profiles. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/120511
url https://hdl.handle.net/10216/120511
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 20726651
10.3390/toxins10070297
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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