Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice

Detalhes bibliográficos
Autor(a) principal: Silva-Carvalho, Ricardo
Data de Publicação: 2019
Outros Autores: Silva, João P., Ferreirinha, Pedro, Leitão, A., Andrade, Fábia K., Costa, Rui M. Gil da, Cristelo, C., Rosa, Morsyleide F., Vilanova, Manuel, Gama, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/58888
Resumo: In view of the growing industrial use of Bacterial cellulose (BC), and taking into account that it might become airborne and be inhaled after industrial processing, assessing its potential pulmonary toxic effects assumes high relevance. In this work, the murine model was used to assess the effects of exposure to respirable BC nanofibrils (nBC), obtained by disintegration of BC produced by Komagataeibacter hansenii. Murine bone marrow-derived macrophages (BMM) were treated with different doses of nBC (0.02 and 0.2 mg/mL, respectively 1 and 10 g of fibrils) in absence or presence of 0.2% Carboxymethyl Cellulose (nBCMC). Furthermore, mice were instilled intratracheally with nBC or nBCMC at different concentrations and at different time-points and analyzed up to 6 months after treatments. Microcrystaline Avicel-plus® CM 2159, a plant-derived cellulose, was used for comparison. Markers of cellular damage (lactate dehydrogenase release and total protein) and oxidative stress (hydrogen peroxidase, reduced glutathione, lipid peroxidation and glutathione peroxidase activity) as well presence of inflammatory cells were evaluated in brochoalveolar lavage (BAL) fluids. Histological analysis of lungs, heart and liver tissues was also performed. BAL analysis showed that exposure to nBCMC or CMC did not induce major alterations in the assessed markers of cell damage, oxidative stress or inflammatory cell numbers in BAL fluid over time, even following cumulative treatments. Avicel-plus® CM 2159 significantly increased LDH release, detected 3 months after 4 weekly administrations. However, histological results revealed a chronic inflammatory response and tissue alterations, being hypertrophy of pulmonary arteries (observed 3 months after nBCMC treatment) of particular concern. These histological alterations remained after 6 months in animals treated with nBC, possibly due to foreign body reaction and the organisms inability to remove the fibers. Overall, despite being a safe and biocompatible biomaterial, BC-derived nanofibrils inhalation may lead to lung pathology and pose significant health risks.
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spelling Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of miceBacterial celluloseAirborne nanofibersLung toxicityInflammationScience & TechnologyIn view of the growing industrial use of Bacterial cellulose (BC), and taking into account that it might become airborne and be inhaled after industrial processing, assessing its potential pulmonary toxic effects assumes high relevance. In this work, the murine model was used to assess the effects of exposure to respirable BC nanofibrils (nBC), obtained by disintegration of BC produced by Komagataeibacter hansenii. Murine bone marrow-derived macrophages (BMM) were treated with different doses of nBC (0.02 and 0.2 mg/mL, respectively 1 and 10 g of fibrils) in absence or presence of 0.2% Carboxymethyl Cellulose (nBCMC). Furthermore, mice were instilled intratracheally with nBC or nBCMC at different concentrations and at different time-points and analyzed up to 6 months after treatments. Microcrystaline Avicel-plus® CM 2159, a plant-derived cellulose, was used for comparison. Markers of cellular damage (lactate dehydrogenase release and total protein) and oxidative stress (hydrogen peroxidase, reduced glutathione, lipid peroxidation and glutathione peroxidase activity) as well presence of inflammatory cells were evaluated in brochoalveolar lavage (BAL) fluids. Histological analysis of lungs, heart and liver tissues was also performed. BAL analysis showed that exposure to nBCMC or CMC did not induce major alterations in the assessed markers of cell damage, oxidative stress or inflammatory cell numbers in BAL fluid over time, even following cumulative treatments. Avicel-plus® CM 2159 significantly increased LDH release, detected 3 months after 4 weekly administrations. However, histological results revealed a chronic inflammatory response and tissue alterations, being hypertrophy of pulmonary arteries (observed 3 months after nBCMC treatment) of particular concern. These histological alterations remained after 6 months in animals treated with nBC, possibly due to foreign body reaction and the organisms inability to remove the fibers. Overall, despite being a safe and biocompatible biomaterial, BC-derived nanofibrils inhalation may lead to lung pathology and pose significant health risks.The authors acknowledge Embrapa Tropical Agroindustry and Coordination for the Improvement of Higher Education Personnel (CAPES) and the project under the bilateral program FCT/CAPES: Bacterial Cellulose: a platform for the development of bionanoproducts for funding this research. This work was also financially supported by: European Investment Funds by FEDER/COMPETE/POCI - Operational Competitiveness and Internationalization Program, under Project POCI-01-0145-FEDER-006958, National Funds by FCT - Portuguese Foundation for Science and Technology, Project POCI-01-0145-FEDER-006939 (Laboratory for Process Engineering, Environment, Biotechnology and Energy - LEPABE funded by FEDER, funds through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) - and by national funds through FCT. Rui Gil da Costa is supported by grant nº SFRH/BPD/85462/2012 from FCT, financed by the Portuguese Government and the Social European Fund. This study was supported by the Portuguese Foundation for Science and Technology (FCT) also under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionKorean Society of ToxicologyUniversidade do MinhoSilva-Carvalho, RicardoSilva, João P.Ferreirinha, PedroLeitão, A.Andrade, Fábia K.Costa, Rui M. Gil daCristelo, C.Rosa, Morsyleide F.Vilanova, ManuelGama, F. M.2019-01-152019-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58888engSilva-Carvalho, Ricardo; Silva, João P.; Ferreirinha, Pedro; Leitão, A.; Andrade, Fábia K.; Costa, Rui M. Gil da; Cristelo, C.; Rosa, Morsyleide F.; Vilanova, Manuel; Gama, F. M., Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice. Toxicological Research, 35(1), 45-63, 20191976-82572234-275310.5487/TR.2019.35.1.045http://www.toxicolres.orginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:07Zoai:repositorium.sdum.uminho.pt:1822/58888Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:49:57.310307Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice
title Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice
spellingShingle Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice
Silva-Carvalho, Ricardo
Bacterial cellulose
Airborne nanofibers
Lung toxicity
Inflammation
Science & Technology
title_short Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice
title_full Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice
title_fullStr Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice
title_full_unstemmed Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice
title_sort Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice
author Silva-Carvalho, Ricardo
author_facet Silva-Carvalho, Ricardo
Silva, João P.
Ferreirinha, Pedro
Leitão, A.
Andrade, Fábia K.
Costa, Rui M. Gil da
Cristelo, C.
Rosa, Morsyleide F.
Vilanova, Manuel
Gama, F. M.
author_role author
author2 Silva, João P.
Ferreirinha, Pedro
Leitão, A.
Andrade, Fábia K.
Costa, Rui M. Gil da
Cristelo, C.
Rosa, Morsyleide F.
Vilanova, Manuel
Gama, F. M.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva-Carvalho, Ricardo
Silva, João P.
Ferreirinha, Pedro
Leitão, A.
Andrade, Fábia K.
Costa, Rui M. Gil da
Cristelo, C.
Rosa, Morsyleide F.
Vilanova, Manuel
Gama, F. M.
dc.subject.por.fl_str_mv Bacterial cellulose
Airborne nanofibers
Lung toxicity
Inflammation
Science & Technology
topic Bacterial cellulose
Airborne nanofibers
Lung toxicity
Inflammation
Science & Technology
description In view of the growing industrial use of Bacterial cellulose (BC), and taking into account that it might become airborne and be inhaled after industrial processing, assessing its potential pulmonary toxic effects assumes high relevance. In this work, the murine model was used to assess the effects of exposure to respirable BC nanofibrils (nBC), obtained by disintegration of BC produced by Komagataeibacter hansenii. Murine bone marrow-derived macrophages (BMM) were treated with different doses of nBC (0.02 and 0.2 mg/mL, respectively 1 and 10 g of fibrils) in absence or presence of 0.2% Carboxymethyl Cellulose (nBCMC). Furthermore, mice were instilled intratracheally with nBC or nBCMC at different concentrations and at different time-points and analyzed up to 6 months after treatments. Microcrystaline Avicel-plus® CM 2159, a plant-derived cellulose, was used for comparison. Markers of cellular damage (lactate dehydrogenase release and total protein) and oxidative stress (hydrogen peroxidase, reduced glutathione, lipid peroxidation and glutathione peroxidase activity) as well presence of inflammatory cells were evaluated in brochoalveolar lavage (BAL) fluids. Histological analysis of lungs, heart and liver tissues was also performed. BAL analysis showed that exposure to nBCMC or CMC did not induce major alterations in the assessed markers of cell damage, oxidative stress or inflammatory cell numbers in BAL fluid over time, even following cumulative treatments. Avicel-plus® CM 2159 significantly increased LDH release, detected 3 months after 4 weekly administrations. However, histological results revealed a chronic inflammatory response and tissue alterations, being hypertrophy of pulmonary arteries (observed 3 months after nBCMC treatment) of particular concern. These histological alterations remained after 6 months in animals treated with nBC, possibly due to foreign body reaction and the organisms inability to remove the fibers. Overall, despite being a safe and biocompatible biomaterial, BC-derived nanofibrils inhalation may lead to lung pathology and pose significant health risks.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-15
2019-01-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/58888
url http://hdl.handle.net/1822/58888
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva-Carvalho, Ricardo; Silva, João P.; Ferreirinha, Pedro; Leitão, A.; Andrade, Fábia K.; Costa, Rui M. Gil da; Cristelo, C.; Rosa, Morsyleide F.; Vilanova, Manuel; Gama, F. M., Inhalation of bacterial cellulose nanofibrils triggers an inflammatory response and changes lung tissue morphology of mice. Toxicological Research, 35(1), 45-63, 2019
1976-8257
2234-2753
10.5487/TR.2019.35.1.045
http://www.toxicolres.org
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Korean Society of Toxicology
publisher.none.fl_str_mv Korean Society of Toxicology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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