Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000200148 |
Resumo: | Abstract Purpose: To evaluate wound contraction and the concentration of mast cells in skin wounds treated with wild plum (Ximenia americana) essential oil-based ointment in rats. Methods: Sixty rats were submitted to two cutaneous wounds in the thoracic region, on the right and left antimeres. Thereon, they were divided into three groups: GX (wounds treated once a day with hydro alcoholic branch extract of Ximenia americana), GP (wounds that received vehicle), and GC (wounds without product application). Wounds were measured immediately after the injury as well as 4, 7, 14 and 21 days post-topical application of the extract. At these days, five rats from each group were euthanatized. Thereafter, samples were fixed in 10% formaldehyde and processed for paraffin embedding. Sections were stained with H.E, Masson's Trichrome and toluidine blue for morphological, morphometrical and histopathological analysis, under light microscopy. The degree of epithelial contraction was measured and mast cell concentrations were also evaluated with an image analyzer (Image Pro-plus®software) . Results: The extract treated group showed lower mast cell concentrations in the 4th day of lesion, as compared to GP (GX<GP=GC, p=0.029), as well as with increased contraction at 7th and 14th days, respectively (7th and 14th days, GX > GP = GC; p<0.05) . Conclusion: Ointment containing 10% X. americana induces a decrease in mast cell concentration, at the beginning of the healing process, and promotes early skin wound contraction in rats. |
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Mast cell concentration and skin wound contraction in rats treated with Ximenia americana LWound HealingPhytotherapyMast CellsSkinRatsAbstract Purpose: To evaluate wound contraction and the concentration of mast cells in skin wounds treated with wild plum (Ximenia americana) essential oil-based ointment in rats. Methods: Sixty rats were submitted to two cutaneous wounds in the thoracic region, on the right and left antimeres. Thereon, they were divided into three groups: GX (wounds treated once a day with hydro alcoholic branch extract of Ximenia americana), GP (wounds that received vehicle), and GC (wounds without product application). Wounds were measured immediately after the injury as well as 4, 7, 14 and 21 days post-topical application of the extract. At these days, five rats from each group were euthanatized. Thereafter, samples were fixed in 10% formaldehyde and processed for paraffin embedding. Sections were stained with H.E, Masson's Trichrome and toluidine blue for morphological, morphometrical and histopathological analysis, under light microscopy. The degree of epithelial contraction was measured and mast cell concentrations were also evaluated with an image analyzer (Image Pro-plus®software) . Results: The extract treated group showed lower mast cell concentrations in the 4th day of lesion, as compared to GP (GX<GP=GC, p=0.029), as well as with increased contraction at 7th and 14th days, respectively (7th and 14th days, GX > GP = GC; p<0.05) . Conclusion: Ointment containing 10% X. americana induces a decrease in mast cell concentration, at the beginning of the healing process, and promotes early skin wound contraction in rats.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000200148Acta Cirúrgica Brasileira v.32 n.2 2017reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020170207info:eu-repo/semantics/openAccessCastro Souza Junior Neto,José deEstevão,Lígia Reis de MouraBaratella-Evêncio,LirianeVieira,Marcela Gabriela FeitosaSimões,Ricardo SantosFlorencio-Silva,RinaldoEvêncio-Luz,LuísEvêncio-Neto,Joaquimeng2017-03-08T00:00:00Zoai:scielo:S0102-86502017000200148Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2017-03-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L |
title |
Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L |
spellingShingle |
Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L Castro Souza Junior Neto,José de Wound Healing Phytotherapy Mast Cells Skin Rats |
title_short |
Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L |
title_full |
Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L |
title_fullStr |
Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L |
title_full_unstemmed |
Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L |
title_sort |
Mast cell concentration and skin wound contraction in rats treated with Ximenia americana L |
author |
Castro Souza Junior Neto,José de |
author_facet |
Castro Souza Junior Neto,José de Estevão,Lígia Reis de Moura Baratella-Evêncio,Liriane Vieira,Marcela Gabriela Feitosa Simões,Ricardo Santos Florencio-Silva,Rinaldo Evêncio-Luz,Luís Evêncio-Neto,Joaquim |
author_role |
author |
author2 |
Estevão,Lígia Reis de Moura Baratella-Evêncio,Liriane Vieira,Marcela Gabriela Feitosa Simões,Ricardo Santos Florencio-Silva,Rinaldo Evêncio-Luz,Luís Evêncio-Neto,Joaquim |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Castro Souza Junior Neto,José de Estevão,Lígia Reis de Moura Baratella-Evêncio,Liriane Vieira,Marcela Gabriela Feitosa Simões,Ricardo Santos Florencio-Silva,Rinaldo Evêncio-Luz,Luís Evêncio-Neto,Joaquim |
dc.subject.por.fl_str_mv |
Wound Healing Phytotherapy Mast Cells Skin Rats |
topic |
Wound Healing Phytotherapy Mast Cells Skin Rats |
description |
Abstract Purpose: To evaluate wound contraction and the concentration of mast cells in skin wounds treated with wild plum (Ximenia americana) essential oil-based ointment in rats. Methods: Sixty rats were submitted to two cutaneous wounds in the thoracic region, on the right and left antimeres. Thereon, they were divided into three groups: GX (wounds treated once a day with hydro alcoholic branch extract of Ximenia americana), GP (wounds that received vehicle), and GC (wounds without product application). Wounds were measured immediately after the injury as well as 4, 7, 14 and 21 days post-topical application of the extract. At these days, five rats from each group were euthanatized. Thereafter, samples were fixed in 10% formaldehyde and processed for paraffin embedding. Sections were stained with H.E, Masson's Trichrome and toluidine blue for morphological, morphometrical and histopathological analysis, under light microscopy. The degree of epithelial contraction was measured and mast cell concentrations were also evaluated with an image analyzer (Image Pro-plus®software) . Results: The extract treated group showed lower mast cell concentrations in the 4th day of lesion, as compared to GP (GX<GP=GC, p=0.029), as well as with increased contraction at 7th and 14th days, respectively (7th and 14th days, GX > GP = GC; p<0.05) . Conclusion: Ointment containing 10% X. americana induces a decrease in mast cell concentration, at the beginning of the healing process, and promotes early skin wound contraction in rats. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000200148 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000200148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0102-865020170207 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.32 n.2 2017 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
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1752126444124766208 |