Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100019 |
Resumo: | PURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress. |
id |
SBDPC-1_1a95938e44c346c64516b401c6c4b190 |
---|---|
oai_identifier_str |
oai:scielo:S0102-86502010000100019 |
network_acronym_str |
SBDPC-1 |
network_name_str |
Acta Cirúrgica Brasileira (Online) |
repository_id_str |
|
spelling |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stressKeratinocytesCell SurvivalOxidative StressPURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100019Acta Cirúrgica Brasileira v.25 n.1 2010reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502010000100019info:eu-repo/semantics/openAccessGragnani,AlfredoRossi,Marina BertelliAlbuquerque,Janne Cely MedeirosBrito,Gabriela Soares SilvaAloise,Antonio CarlosFerreira,Lydia Masakoeng2010-01-19T00:00:00Zoai:scielo:S0102-86502010000100019Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2010-01-19T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress |
title |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress |
spellingShingle |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress Gragnani,Alfredo Keratinocytes Cell Survival Oxidative Stress |
title_short |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress |
title_full |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress |
title_fullStr |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress |
title_full_unstemmed |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress |
title_sort |
Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress |
author |
Gragnani,Alfredo |
author_facet |
Gragnani,Alfredo Rossi,Marina Bertelli Albuquerque,Janne Cely Medeiros Brito,Gabriela Soares Silva Aloise,Antonio Carlos Ferreira,Lydia Masako |
author_role |
author |
author2 |
Rossi,Marina Bertelli Albuquerque,Janne Cely Medeiros Brito,Gabriela Soares Silva Aloise,Antonio Carlos Ferreira,Lydia Masako |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gragnani,Alfredo Rossi,Marina Bertelli Albuquerque,Janne Cely Medeiros Brito,Gabriela Soares Silva Aloise,Antonio Carlos Ferreira,Lydia Masako |
dc.subject.por.fl_str_mv |
Keratinocytes Cell Survival Oxidative Stress |
topic |
Keratinocytes Cell Survival Oxidative Stress |
description |
PURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502010000100019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.25 n.1 2010 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126439926267904 |