Does mesenchymal stem cell improve the liver regeneration after the 70% hepatectomy?

• Autor(a) principal: Alves,Ana Karina Soares
• Data de Publicação: 2017
• Outros Autores: Lanzoni,Valéria, Fuziy,Rogério Aoki, Franco,Rita Maria Aparecida Monteiro Moura, Maeda,Carlos Toshinori, Lopes Filho,Gaspar de Jesus, Linhares,Marcelo Moura
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Cirúrgica Brasileira (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000700515
• topic: Mesenchymal Stem Cell Transplantation; Liver Regeneration; Hepatectomy; Cell- and Tissue-Based Therapy; Rats

Abstract Purpose: To evaluate the effects of mesenchymal stem cells on liver regeneration in rats following a 70% hepatectomy. Methods: Forty rats were subjected to 70% hepatectomy and then ~106 mesenchymal stem cells (test group), or saline solution (control group), were infused into their livers via the portal vein. Each treatment group was divided into early and late subgroups (euthanized 3 d and 5 d following the operation, respectively). Group comparisons of Albumin, aminotransaminases (AST, ALT), and Alcaline Phosphatase (AP) levels, proliferative index (ki-67+ straining), and mitotic cell counts were conducted. Results: No significant differences in liver regeneration rate, number of mitoses, proliferative index, or serum levels of albumin, AST, or AP were observed. ALT levels were higher in the test group than in the control group (p<.05). Conclusions: Mesenchymal stem-cell therapy did not improve liver regeneration rate 3 d or 5 d after 70% hepatectomy in rats. Likewise, the therapy appeared not to affect liver function, proliferative index, or number of mitoses significantly.