Effect of aging on liver regeneration in rats
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006000400002 |
Resumo: | PURPOSE: Regeneration and/or healing of tissues is believed to be more difficult in elderly people. The liver is one of the most complex organs in the human body, and is involved in a variety of functions. Liver regeneration is the body's protection mechanism against loss of functional liver tissue. The aim of this study is to identify the regenerative capacity of the liver in older animals and to compare it with that of young adult animals. METHODS: Thirty-four Wistar rats were used, of which 17 were 90 days old (young animals) and 17 were 460 days old (old animals). Approximately 70% of the liver was surgically removed. Examinations were carried out after 24 hours and on day 7, using 3 methods: KWON et al.'s formula to identify increase in volume; mitotic figure count in 5 fields; and the percentage of PCNA-positive nuclei in 5 fields. RESULTS: The increase in volume of the remaining liver was greater in the young animals after both 24 hours (p=0.0006) and on day 7 (p=0.0000). Histological cuts showed a greater mitotic figure count in young animals evaluated after 24 hours (p=0.0000). Upon evaluation on day 7, recovery was observed in the old animals. This recovery was similar to that of the young ones (p=0.2851). The PCNA-positive nucleus count was greater in the young animals' liver cuts after 24 hours (p=0.0310), and, while it had decreased in young animals by day 7, recovery was observed in the older animals (p=0.0298). CONCLUSION: The data confirm that age is related to delay in liver regeneration in rats. |
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Acta Cirúrgica Brasileira (Online) |
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Effect of aging on liver regeneration in ratsLiver RegenerationAgingHepatectomyPURPOSE: Regeneration and/or healing of tissues is believed to be more difficult in elderly people. The liver is one of the most complex organs in the human body, and is involved in a variety of functions. Liver regeneration is the body's protection mechanism against loss of functional liver tissue. The aim of this study is to identify the regenerative capacity of the liver in older animals and to compare it with that of young adult animals. METHODS: Thirty-four Wistar rats were used, of which 17 were 90 days old (young animals) and 17 were 460 days old (old animals). Approximately 70% of the liver was surgically removed. Examinations were carried out after 24 hours and on day 7, using 3 methods: KWON et al.'s formula to identify increase in volume; mitotic figure count in 5 fields; and the percentage of PCNA-positive nuclei in 5 fields. RESULTS: The increase in volume of the remaining liver was greater in the young animals after both 24 hours (p=0.0006) and on day 7 (p=0.0000). Histological cuts showed a greater mitotic figure count in young animals evaluated after 24 hours (p=0.0000). Upon evaluation on day 7, recovery was observed in the old animals. This recovery was similar to that of the young ones (p=0.2851). The PCNA-positive nucleus count was greater in the young animals' liver cuts after 24 hours (p=0.0310), and, while it had decreased in young animals by day 7, recovery was observed in the older animals (p=0.0298). CONCLUSION: The data confirm that age is related to delay in liver regeneration in rats.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006000400002Acta Cirúrgica Brasileira v.21 n.4 2006reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502006000400002info:eu-repo/semantics/openAccessBiondo-Simões,Maria de Lourdes PessoleMatias,Jorge Eduardo FoutoMontibeller,Guilherme RaminaSiqueira,Letícia Cristina DalledoneNunes,Eduardo da SilvaGrassi,Cristiano Antonioeng2006-09-20T00:00:00Zoai:scielo:S0102-86502006000400002Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2006-09-20T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Effect of aging on liver regeneration in rats |
title |
Effect of aging on liver regeneration in rats |
spellingShingle |
Effect of aging on liver regeneration in rats Biondo-Simões,Maria de Lourdes Pessole Liver Regeneration Aging Hepatectomy |
title_short |
Effect of aging on liver regeneration in rats |
title_full |
Effect of aging on liver regeneration in rats |
title_fullStr |
Effect of aging on liver regeneration in rats |
title_full_unstemmed |
Effect of aging on liver regeneration in rats |
title_sort |
Effect of aging on liver regeneration in rats |
author |
Biondo-Simões,Maria de Lourdes Pessole |
author_facet |
Biondo-Simões,Maria de Lourdes Pessole Matias,Jorge Eduardo Fouto Montibeller,Guilherme Ramina Siqueira,Letícia Cristina Dalledone Nunes,Eduardo da Silva Grassi,Cristiano Antonio |
author_role |
author |
author2 |
Matias,Jorge Eduardo Fouto Montibeller,Guilherme Ramina Siqueira,Letícia Cristina Dalledone Nunes,Eduardo da Silva Grassi,Cristiano Antonio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Biondo-Simões,Maria de Lourdes Pessole Matias,Jorge Eduardo Fouto Montibeller,Guilherme Ramina Siqueira,Letícia Cristina Dalledone Nunes,Eduardo da Silva Grassi,Cristiano Antonio |
dc.subject.por.fl_str_mv |
Liver Regeneration Aging Hepatectomy |
topic |
Liver Regeneration Aging Hepatectomy |
description |
PURPOSE: Regeneration and/or healing of tissues is believed to be more difficult in elderly people. The liver is one of the most complex organs in the human body, and is involved in a variety of functions. Liver regeneration is the body's protection mechanism against loss of functional liver tissue. The aim of this study is to identify the regenerative capacity of the liver in older animals and to compare it with that of young adult animals. METHODS: Thirty-four Wistar rats were used, of which 17 were 90 days old (young animals) and 17 were 460 days old (old animals). Approximately 70% of the liver was surgically removed. Examinations were carried out after 24 hours and on day 7, using 3 methods: KWON et al.'s formula to identify increase in volume; mitotic figure count in 5 fields; and the percentage of PCNA-positive nuclei in 5 fields. RESULTS: The increase in volume of the remaining liver was greater in the young animals after both 24 hours (p=0.0006) and on day 7 (p=0.0000). Histological cuts showed a greater mitotic figure count in young animals evaluated after 24 hours (p=0.0000). Upon evaluation on day 7, recovery was observed in the old animals. This recovery was similar to that of the young ones (p=0.2851). The PCNA-positive nucleus count was greater in the young animals' liver cuts after 24 hours (p=0.0310), and, while it had decreased in young animals by day 7, recovery was observed in the older animals (p=0.0298). CONCLUSION: The data confirm that age is related to delay in liver regeneration in rats. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006000400002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006000400002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502006000400002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.21 n.4 2006 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126438147883008 |