Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab

Bibliographic Details
Main Author: Lopes,João Vieira
Publication Date: 2008
Other Authors: Freitas,Luís Alberto Mendonça de, Marques,Ravi Dias, Bocca,Anamélia Lorenzetti, Sousa,João Batista de, Oliveira,Paulo Gonçalves de
Format: Article
Language: eng
Source: Acta Cirúrgica Brasileira (Online)
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000500009
Summary: PURPOSE: To evaluate the effects of infliximab, a murine/human chimeric monoclonal antibody, on the tensile strength of abdominal wall surgical wounds. METHODS: Sixty Wistar healthy male rats with initial body weight between 215 and 390 g and 60 and 90 days of age were randomly assigned into two groups, E (Experimental) and C (Control) with 30 animals each. Group E animals received a single subcutaneous dose of 5mg/Kg of infliximab, and Group C animals received equivalent subcutaneous volume of a solution of 0.9% NaCl. After 48h, animals from both groups were submitted to a 4 cm median incision in the abdominal wall, including all layers that had been reconstituted with continuous suture of the aponeurotic muscle and skin, with 5.0 nylon thread. Then, Group E animals were separated by simple allotment into three subgroups named E3, E7 and E14 with ten animals each, and those from group C into C3, C7, C14 and were submitted, respectively, the reoperation and euthanasia at the third, seventh and fourteenth postoperative day. The anterior abdominal wall, which was resected during reoperation, was cut with No 15 scalpel lamina perpendicularly to the surgical wound. Each specimen, in the form of a 6 cm x 2 cm strip, was fixed by the extremity so that the suture line was equidistant from the fixation points of the dynamometer, in order to undergo the tensile strength test. The dynamometer, which was gauged for each series of measures, was calibrated to apply velocity to the 25 mm/min rupture test; the rupture value was expressed in N (Newton). Prior to euthanasia, the abdominal vena cava was identified and punctured in order to collect blood for TNF-α dosage. RESULTS: The mean tensile strength found for animals from subgroups E3, E7, E14, C3, C7, C14 were, respectively, 16.03, 18.69, 27.01, 28.40, 27.22, 29.15 and 24.30 N. In the results of the multiple comparisons tests, significant differences (p<0.05) was found between subgroups E3 and E7 compared with C3, C7 and C14. CONCLUSION: The infliximab interfered in the healing of the abdominal wall wound decreasing the rupture strength in the inflammatory and proliferative phases.
id SBDPC-1_e84677fcdbe7259e054fafa758fbf39b
oai_identifier_str oai:scielo:S0102-86502008000500009
network_acronym_str SBDPC-1
network_name_str Acta Cirúrgica Brasileira (Online)
repository_id_str
spelling Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximabAntibodies, MonoclonalAbdominal WallWound HealingRatsPURPOSE: To evaluate the effects of infliximab, a murine/human chimeric monoclonal antibody, on the tensile strength of abdominal wall surgical wounds. METHODS: Sixty Wistar healthy male rats with initial body weight between 215 and 390 g and 60 and 90 days of age were randomly assigned into two groups, E (Experimental) and C (Control) with 30 animals each. Group E animals received a single subcutaneous dose of 5mg/Kg of infliximab, and Group C animals received equivalent subcutaneous volume of a solution of 0.9% NaCl. After 48h, animals from both groups were submitted to a 4 cm median incision in the abdominal wall, including all layers that had been reconstituted with continuous suture of the aponeurotic muscle and skin, with 5.0 nylon thread. Then, Group E animals were separated by simple allotment into three subgroups named E3, E7 and E14 with ten animals each, and those from group C into C3, C7, C14 and were submitted, respectively, the reoperation and euthanasia at the third, seventh and fourteenth postoperative day. The anterior abdominal wall, which was resected during reoperation, was cut with No 15 scalpel lamina perpendicularly to the surgical wound. Each specimen, in the form of a 6 cm x 2 cm strip, was fixed by the extremity so that the suture line was equidistant from the fixation points of the dynamometer, in order to undergo the tensile strength test. The dynamometer, which was gauged for each series of measures, was calibrated to apply velocity to the 25 mm/min rupture test; the rupture value was expressed in N (Newton). Prior to euthanasia, the abdominal vena cava was identified and punctured in order to collect blood for TNF-α dosage. RESULTS: The mean tensile strength found for animals from subgroups E3, E7, E14, C3, C7, C14 were, respectively, 16.03, 18.69, 27.01, 28.40, 27.22, 29.15 and 24.30 N. In the results of the multiple comparisons tests, significant differences (p<0.05) was found between subgroups E3 and E7 compared with C3, C7 and C14. CONCLUSION: The infliximab interfered in the healing of the abdominal wall wound decreasing the rupture strength in the inflammatory and proliferative phases.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000500009Acta Cirúrgica Brasileira v.23 n.5 2008reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502008000500009info:eu-repo/semantics/openAccessLopes,João VieiraFreitas,Luís Alberto Mendonça deMarques,Ravi DiasBocca,Anamélia LorenzettiSousa,João Batista deOliveira,Paulo Gonçalves deeng2008-09-03T00:00:00Zoai:scielo:S0102-86502008000500009Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2008-09-03T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab
title Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab
spellingShingle Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab
Lopes,João Vieira
Antibodies, Monoclonal
Abdominal Wall
Wound Healing
Rats
title_short Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab
title_full Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab
title_fullStr Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab
title_full_unstemmed Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab
title_sort Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab
author Lopes,João Vieira
author_facet Lopes,João Vieira
Freitas,Luís Alberto Mendonça de
Marques,Ravi Dias
Bocca,Anamélia Lorenzetti
Sousa,João Batista de
Oliveira,Paulo Gonçalves de
author_role author
author2 Freitas,Luís Alberto Mendonça de
Marques,Ravi Dias
Bocca,Anamélia Lorenzetti
Sousa,João Batista de
Oliveira,Paulo Gonçalves de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lopes,João Vieira
Freitas,Luís Alberto Mendonça de
Marques,Ravi Dias
Bocca,Anamélia Lorenzetti
Sousa,João Batista de
Oliveira,Paulo Gonçalves de
dc.subject.por.fl_str_mv Antibodies, Monoclonal
Abdominal Wall
Wound Healing
Rats
topic Antibodies, Monoclonal
Abdominal Wall
Wound Healing
Rats
description PURPOSE: To evaluate the effects of infliximab, a murine/human chimeric monoclonal antibody, on the tensile strength of abdominal wall surgical wounds. METHODS: Sixty Wistar healthy male rats with initial body weight between 215 and 390 g and 60 and 90 days of age were randomly assigned into two groups, E (Experimental) and C (Control) with 30 animals each. Group E animals received a single subcutaneous dose of 5mg/Kg of infliximab, and Group C animals received equivalent subcutaneous volume of a solution of 0.9% NaCl. After 48h, animals from both groups were submitted to a 4 cm median incision in the abdominal wall, including all layers that had been reconstituted with continuous suture of the aponeurotic muscle and skin, with 5.0 nylon thread. Then, Group E animals were separated by simple allotment into three subgroups named E3, E7 and E14 with ten animals each, and those from group C into C3, C7, C14 and were submitted, respectively, the reoperation and euthanasia at the third, seventh and fourteenth postoperative day. The anterior abdominal wall, which was resected during reoperation, was cut with No 15 scalpel lamina perpendicularly to the surgical wound. Each specimen, in the form of a 6 cm x 2 cm strip, was fixed by the extremity so that the suture line was equidistant from the fixation points of the dynamometer, in order to undergo the tensile strength test. The dynamometer, which was gauged for each series of measures, was calibrated to apply velocity to the 25 mm/min rupture test; the rupture value was expressed in N (Newton). Prior to euthanasia, the abdominal vena cava was identified and punctured in order to collect blood for TNF-α dosage. RESULTS: The mean tensile strength found for animals from subgroups E3, E7, E14, C3, C7, C14 were, respectively, 16.03, 18.69, 27.01, 28.40, 27.22, 29.15 and 24.30 N. In the results of the multiple comparisons tests, significant differences (p<0.05) was found between subgroups E3 and E7 compared with C3, C7 and C14. CONCLUSION: The infliximab interfered in the healing of the abdominal wall wound decreasing the rupture strength in the inflammatory and proliferative phases.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000500009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502008000500009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502008000500009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.23 n.5 2008
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
_version_ 1752126439165001728