Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000600702 |
Resumo: | ABSTRACT In general, Passiflora species have been reported for their folk medicinal use as sedative and anti-inflammatory. However, P. caerulea has already been reported to treat pulmonary diseases. Severe pulmonary tuberculosis, generally caused by Mycobacterium tuberculosis strains resistant to multiple drugs, can lead to deleterious inflammation and high mortality, encouraging new approaches in drug discovery. Thus, the aim of this work was to evaluate the Passiflora mucronata Lam., Passifloraceae, potential for tuberculosis treatment. Specifically, related to antimycobacterial activity and anti-inflammatory related effects (based on inhibition of nitric oxide, tumor necrosis factor-alpha production and antioxidant potential), as well as the chemical profile of P. mucronata. High performance liquid chromatography coupled with diode-array ultraviolet and mass spectrometer analyses of crude hydroalcoholic extract and ethyl acetate fraction showed the presence of flavonoids. Ethyl acetate fraction showed to be as antioxidant as Ginkgo biloba standard extract with EC50 of 14.61 ± 1.25 µg/ml. One major flavonoid isolated from ethyl acetate fraction was characterized as isoorientin. The hexane fraction and its main isolated compound, the triterpene β-amyrin, exhibited significant growth inhibitory activity against Mycobacterium bovis BCG (MIC50 1.61 ± 1.43 and 3.93 ± 1.05 µg/ml, respectively). In addition, Passiflora mucronata samples, specially hexane and dichloromethane fractions, as well as pure β-amyrin, showed a dose-related inhibition of lipopolysaccharide (LPS)-induced nitric oxide production. In conclusion, Passiflora mucronata presented relevant biological potential and should be considered for further studies using in vivo pulmonary tuberculosis model. |
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Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restingaPassifloraceaeTerpenesFlavonoidAntimycobacterialImmunomodulatoryAntioxidantABSTRACT In general, Passiflora species have been reported for their folk medicinal use as sedative and anti-inflammatory. However, P. caerulea has already been reported to treat pulmonary diseases. Severe pulmonary tuberculosis, generally caused by Mycobacterium tuberculosis strains resistant to multiple drugs, can lead to deleterious inflammation and high mortality, encouraging new approaches in drug discovery. Thus, the aim of this work was to evaluate the Passiflora mucronata Lam., Passifloraceae, potential for tuberculosis treatment. Specifically, related to antimycobacterial activity and anti-inflammatory related effects (based on inhibition of nitric oxide, tumor necrosis factor-alpha production and antioxidant potential), as well as the chemical profile of P. mucronata. High performance liquid chromatography coupled with diode-array ultraviolet and mass spectrometer analyses of crude hydroalcoholic extract and ethyl acetate fraction showed the presence of flavonoids. Ethyl acetate fraction showed to be as antioxidant as Ginkgo biloba standard extract with EC50 of 14.61 ± 1.25 µg/ml. One major flavonoid isolated from ethyl acetate fraction was characterized as isoorientin. The hexane fraction and its main isolated compound, the triterpene β-amyrin, exhibited significant growth inhibitory activity against Mycobacterium bovis BCG (MIC50 1.61 ± 1.43 and 3.93 ± 1.05 µg/ml, respectively). In addition, Passiflora mucronata samples, specially hexane and dichloromethane fractions, as well as pure β-amyrin, showed a dose-related inhibition of lipopolysaccharide (LPS)-induced nitric oxide production. In conclusion, Passiflora mucronata presented relevant biological potential and should be considered for further studies using in vivo pulmonary tuberculosis model.Sociedade Brasileira de Farmacognosia2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000600702Revista Brasileira de Farmacognosia v.27 n.6 2017reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1016/j.bjp.2017.07.005info:eu-repo/semantics/openAccessAraujo,Marlon H. deSilva,Isabel C.V. daOliveira,Pollyana F. deBarreto,Arielly R.R.Konno,Tatiana U.P.Esteves,Francisco de A.Barth,ThiagoAguiar,Fernando A.Lopes,Norberto P.Dermenjian,Renee K.Guimarães,Denise O.Leal,Ivana C.R.Lasunskaia,Elena B.Muzitano,Michelle Frazãoeng2018-01-08T00:00:00Zoai:scielo:S0102-695X2017000600702Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2018-01-08T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga |
title |
Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga |
spellingShingle |
Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga Araujo,Marlon H. de Passifloraceae Terpenes Flavonoid Antimycobacterial Immunomodulatory Antioxidant |
title_short |
Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga |
title_full |
Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga |
title_fullStr |
Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga |
title_full_unstemmed |
Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga |
title_sort |
Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga |
author |
Araujo,Marlon H. de |
author_facet |
Araujo,Marlon H. de Silva,Isabel C.V. da Oliveira,Pollyana F. de Barreto,Arielly R.R. Konno,Tatiana U.P. Esteves,Francisco de A. Barth,Thiago Aguiar,Fernando A. Lopes,Norberto P. Dermenjian,Renee K. Guimarães,Denise O. Leal,Ivana C.R. Lasunskaia,Elena B. Muzitano,Michelle Frazão |
author_role |
author |
author2 |
Silva,Isabel C.V. da Oliveira,Pollyana F. de Barreto,Arielly R.R. Konno,Tatiana U.P. Esteves,Francisco de A. Barth,Thiago Aguiar,Fernando A. Lopes,Norberto P. Dermenjian,Renee K. Guimarães,Denise O. Leal,Ivana C.R. Lasunskaia,Elena B. Muzitano,Michelle Frazão |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Araujo,Marlon H. de Silva,Isabel C.V. da Oliveira,Pollyana F. de Barreto,Arielly R.R. Konno,Tatiana U.P. Esteves,Francisco de A. Barth,Thiago Aguiar,Fernando A. Lopes,Norberto P. Dermenjian,Renee K. Guimarães,Denise O. Leal,Ivana C.R. Lasunskaia,Elena B. Muzitano,Michelle Frazão |
dc.subject.por.fl_str_mv |
Passifloraceae Terpenes Flavonoid Antimycobacterial Immunomodulatory Antioxidant |
topic |
Passifloraceae Terpenes Flavonoid Antimycobacterial Immunomodulatory Antioxidant |
description |
ABSTRACT In general, Passiflora species have been reported for their folk medicinal use as sedative and anti-inflammatory. However, P. caerulea has already been reported to treat pulmonary diseases. Severe pulmonary tuberculosis, generally caused by Mycobacterium tuberculosis strains resistant to multiple drugs, can lead to deleterious inflammation and high mortality, encouraging new approaches in drug discovery. Thus, the aim of this work was to evaluate the Passiflora mucronata Lam., Passifloraceae, potential for tuberculosis treatment. Specifically, related to antimycobacterial activity and anti-inflammatory related effects (based on inhibition of nitric oxide, tumor necrosis factor-alpha production and antioxidant potential), as well as the chemical profile of P. mucronata. High performance liquid chromatography coupled with diode-array ultraviolet and mass spectrometer analyses of crude hydroalcoholic extract and ethyl acetate fraction showed the presence of flavonoids. Ethyl acetate fraction showed to be as antioxidant as Ginkgo biloba standard extract with EC50 of 14.61 ± 1.25 µg/ml. One major flavonoid isolated from ethyl acetate fraction was characterized as isoorientin. The hexane fraction and its main isolated compound, the triterpene β-amyrin, exhibited significant growth inhibitory activity against Mycobacterium bovis BCG (MIC50 1.61 ± 1.43 and 3.93 ± 1.05 µg/ml, respectively). In addition, Passiflora mucronata samples, specially hexane and dichloromethane fractions, as well as pure β-amyrin, showed a dose-related inhibition of lipopolysaccharide (LPS)-induced nitric oxide production. In conclusion, Passiflora mucronata presented relevant biological potential and should be considered for further studies using in vivo pulmonary tuberculosis model. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000600702 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000600702 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjp.2017.07.005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.27 n.6 2017 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
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SBFGNOSIA |
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SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
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1752122470584811520 |