Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes)
Main Author: | |
---|---|
Publication Date: | 2014 |
Other Authors: | , |
Format: | Article |
Language: | eng |
Source: | Genetics and Molecular Biology |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100009 |
Summary: | The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade of the Serrasalmidae family: Colossoma macropomum, Mylossoma aureum, M. duriventre, and Piaractus mesopotamicus, in order to understand the chromosomal evolution in the family. All the species studied had diploid numbers 2n = 54 and exclusively biarmed chromosomes, but variations of the karyotypic formulas were observed. C-banding resulted in similar patterns among the analyzed species, with heterochromatic blocks mainly present in centromeric regions. The 18S rDNA mapping of C. macropomum and P. mesopotamicus revealed multiple sites of this gene; 5S rDNA sites were detected in two chromosome pairs in all species, although not all of them were homeologs. Hybridization with a telomeric probe revealed signals in the terminal portions of chromosomes in all the species and an interstitial signal was observed in one pair of C. macropomum. |
id |
SBG-1_1e4a566627ec6c936991644943e5e558 |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572014000100009 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes)5S rDNAtelomeric sequencestambaqui and pacuThe Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade of the Serrasalmidae family: Colossoma macropomum, Mylossoma aureum, M. duriventre, and Piaractus mesopotamicus, in order to understand the chromosomal evolution in the family. All the species studied had diploid numbers 2n = 54 and exclusively biarmed chromosomes, but variations of the karyotypic formulas were observed. C-banding resulted in similar patterns among the analyzed species, with heterochromatic blocks mainly present in centromeric regions. The 18S rDNA mapping of C. macropomum and P. mesopotamicus revealed multiple sites of this gene; 5S rDNA sites were detected in two chromosome pairs in all species, although not all of them were homeologs. Hybridization with a telomeric probe revealed signals in the terminal portions of chromosomes in all the species and an interstitial signal was observed in one pair of C. macropomum.Sociedade Brasileira de Genética2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100009Genetics and Molecular Biology v.37 n.1 2014reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572014000100009info:eu-repo/semantics/openAccessRibeiro,Leila BragaMatoso,Daniele AparecidaFeldberg,Elianaeng2014-03-24T00:00:00Zoai:scielo:S1415-47572014000100009Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2014-03-24T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) |
title |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) |
spellingShingle |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) Ribeiro,Leila Braga 5S rDNA telomeric sequences tambaqui and pacu |
title_short |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) |
title_full |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) |
title_fullStr |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) |
title_full_unstemmed |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) |
title_sort |
Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) |
author |
Ribeiro,Leila Braga |
author_facet |
Ribeiro,Leila Braga Matoso,Daniele Aparecida Feldberg,Eliana |
author_role |
author |
author2 |
Matoso,Daniele Aparecida Feldberg,Eliana |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ribeiro,Leila Braga Matoso,Daniele Aparecida Feldberg,Eliana |
dc.subject.por.fl_str_mv |
5S rDNA telomeric sequences tambaqui and pacu |
topic |
5S rDNA telomeric sequences tambaqui and pacu |
description |
The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade of the Serrasalmidae family: Colossoma macropomum, Mylossoma aureum, M. duriventre, and Piaractus mesopotamicus, in order to understand the chromosomal evolution in the family. All the species studied had diploid numbers 2n = 54 and exclusively biarmed chromosomes, but variations of the karyotypic formulas were observed. C-banding resulted in similar patterns among the analyzed species, with heterochromatic blocks mainly present in centromeric regions. The 18S rDNA mapping of C. macropomum and P. mesopotamicus revealed multiple sites of this gene; 5S rDNA sites were detected in two chromosome pairs in all species, although not all of them were homeologs. Hybridization with a telomeric probe revealed signals in the terminal portions of chromosomes in all the species and an interstitial signal was observed in one pair of C. macropomum. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572014000100009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.37 n.1 2014 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122385824219136 |