Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100020 |
Resumo: | We used agromorphological and random amplified polymorphic DNA (RAPD) molecular marker data to identify duplicate common bean (Phaseolus vulgaris L. Fabaceae) accessions in the Common Bean Germplasm Bank of the Agronomical Institute - IAC (Banco de Germoplasma de feijoeiro do Instituto Agronômico de Campinas (IAC), SP, Brazil). A total of 116 accessions with the same names and similar agromorphological traits was analyzed. The divergence between the accessions was initially evaluated by means of the agromorphological descriptors using single linkage clustering, from the Euclidean distance. Multivariate analysis identified four duplicate accessions (Carioca Lustroso, Bico de Ouro, Jamapa and Preto), with 17 other same-name accessions being suspected duplicates due to their low divergence levels. Accessions with low genetic distance values (indicating that they were duplicates) were further compared using RAPD markers which confirmed the results of the multivariate analyses in relation to the four duplicate accessions, although only two of the other 17 suspect accessions were confirmed to be duplicates, in this case of accessions IAPAR 57 and Sacavem. These results show that the combined use of agromorphological and molecular information allowed a better characterization of the acessions in the common bean Germplasm Bank. |
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Genetics and Molecular Biology |
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Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular dataPhaseolus vulgarismultivariate analysisgermplasm banksdescriptorsWe used agromorphological and random amplified polymorphic DNA (RAPD) molecular marker data to identify duplicate common bean (Phaseolus vulgaris L. Fabaceae) accessions in the Common Bean Germplasm Bank of the Agronomical Institute - IAC (Banco de Germoplasma de feijoeiro do Instituto Agronômico de Campinas (IAC), SP, Brazil). A total of 116 accessions with the same names and similar agromorphological traits was analyzed. The divergence between the accessions was initially evaluated by means of the agromorphological descriptors using single linkage clustering, from the Euclidean distance. Multivariate analysis identified four duplicate accessions (Carioca Lustroso, Bico de Ouro, Jamapa and Preto), with 17 other same-name accessions being suspected duplicates due to their low divergence levels. Accessions with low genetic distance values (indicating that they were duplicates) were further compared using RAPD markers which confirmed the results of the multivariate analyses in relation to the four duplicate accessions, although only two of the other 17 suspect accessions were confirmed to be duplicates, in this case of accessions IAPAR 57 and Sacavem. These results show that the combined use of agromorphological and molecular information allowed a better characterization of the acessions in the common bean Germplasm Bank.Sociedade Brasileira de Genética2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100020Genetics and Molecular Biology v.29 n.1 2006reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572006000100020info:eu-repo/semantics/openAccessChiorato,Alisson FernandoCarbonell,Sérgio Augusto MoraisDias,Luiz Antonio dos SantosMoura,Rafaeli RamosChiavegato,Marilia BarbosaColombo,Carlos Augustoeng2006-03-10T00:00:00Zoai:scielo:S1415-47572006000100020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2006-03-10T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data |
title |
Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data |
spellingShingle |
Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data Chiorato,Alisson Fernando Phaseolus vulgaris multivariate analysis germplasm banks descriptors |
title_short |
Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data |
title_full |
Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data |
title_fullStr |
Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data |
title_full_unstemmed |
Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data |
title_sort |
Identification of common bean (Phaseolus vulgaris) duplicates using agromorphological and molecular data |
author |
Chiorato,Alisson Fernando |
author_facet |
Chiorato,Alisson Fernando Carbonell,Sérgio Augusto Morais Dias,Luiz Antonio dos Santos Moura,Rafaeli Ramos Chiavegato,Marilia Barbosa Colombo,Carlos Augusto |
author_role |
author |
author2 |
Carbonell,Sérgio Augusto Morais Dias,Luiz Antonio dos Santos Moura,Rafaeli Ramos Chiavegato,Marilia Barbosa Colombo,Carlos Augusto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Chiorato,Alisson Fernando Carbonell,Sérgio Augusto Morais Dias,Luiz Antonio dos Santos Moura,Rafaeli Ramos Chiavegato,Marilia Barbosa Colombo,Carlos Augusto |
dc.subject.por.fl_str_mv |
Phaseolus vulgaris multivariate analysis germplasm banks descriptors |
topic |
Phaseolus vulgaris multivariate analysis germplasm banks descriptors |
description |
We used agromorphological and random amplified polymorphic DNA (RAPD) molecular marker data to identify duplicate common bean (Phaseolus vulgaris L. Fabaceae) accessions in the Common Bean Germplasm Bank of the Agronomical Institute - IAC (Banco de Germoplasma de feijoeiro do Instituto Agronômico de Campinas (IAC), SP, Brazil). A total of 116 accessions with the same names and similar agromorphological traits was analyzed. The divergence between the accessions was initially evaluated by means of the agromorphological descriptors using single linkage clustering, from the Euclidean distance. Multivariate analysis identified four duplicate accessions (Carioca Lustroso, Bico de Ouro, Jamapa and Preto), with 17 other same-name accessions being suspected duplicates due to their low divergence levels. Accessions with low genetic distance values (indicating that they were duplicates) were further compared using RAPD markers which confirmed the results of the multivariate analyses in relation to the four duplicate accessions, although only two of the other 17 suspect accessions were confirmed to be duplicates, in this case of accessions IAPAR 57 and Sacavem. These results show that the combined use of agromorphological and molecular information allowed a better characterization of the acessions in the common bean Germplasm Bank. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572006000100020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.29 n.1 2006 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122379869356032 |