ESTs and putative line-specific (broiler and layer) SNPs identified in genes expressed in Gallus gallus pituitary and hypothalamus

Detalhes bibliográficos
Autor(a) principal: Cassoli,Clarissa Sanches da Silva
Data de Publicação: 2007
Outros Autores: Jorge,Erika Cirstina, Patrício,Mateus, Alves,Helena Javiel, Almeida,Erik Amazonas de, Ledur,Mônica Corrêa, Coutinho,Luiz Lehmann
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008
Resumo: Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single nucleotide polymorphisms (SNPs). The collection of expressed sequence tags (ESTs) and SNPs identified in this study represents an important resource for future studies aimed at identifying genes responsible for growth in chicken.