Comparison of three decontamination methods for Mycobacterium bovis isolation
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200008 |
Resumo: | Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method. |
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Brazilian Journal of Microbiology |
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Comparison of three decontamination methods for Mycobacterium bovis isolationMycobacterium bovisdecontaminationPetroffHPCSixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.Sociedade Brasileira de Microbiologia2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200008Brazilian Journal of Microbiology v.39 n.2 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000200008info:eu-repo/semantics/openAccessAmbrosio,Simone RodriguesOliveira,Eugenia Márcia de DeusRodriguez,Cesar Alejandro RosalesFerreira Neto,José SoaresAmaku,Marcoseng2008-07-31T00:00:00Zoai:scielo:S1517-83822008000200008Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-07-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Comparison of three decontamination methods for Mycobacterium bovis isolation |
title |
Comparison of three decontamination methods for Mycobacterium bovis isolation |
spellingShingle |
Comparison of three decontamination methods for Mycobacterium bovis isolation Ambrosio,Simone Rodrigues Mycobacterium bovis decontamination Petroff HPC |
title_short |
Comparison of three decontamination methods for Mycobacterium bovis isolation |
title_full |
Comparison of three decontamination methods for Mycobacterium bovis isolation |
title_fullStr |
Comparison of three decontamination methods for Mycobacterium bovis isolation |
title_full_unstemmed |
Comparison of three decontamination methods for Mycobacterium bovis isolation |
title_sort |
Comparison of three decontamination methods for Mycobacterium bovis isolation |
author |
Ambrosio,Simone Rodrigues |
author_facet |
Ambrosio,Simone Rodrigues Oliveira,Eugenia Márcia de Deus Rodriguez,Cesar Alejandro Rosales Ferreira Neto,José Soares Amaku,Marcos |
author_role |
author |
author2 |
Oliveira,Eugenia Márcia de Deus Rodriguez,Cesar Alejandro Rosales Ferreira Neto,José Soares Amaku,Marcos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ambrosio,Simone Rodrigues Oliveira,Eugenia Márcia de Deus Rodriguez,Cesar Alejandro Rosales Ferreira Neto,José Soares Amaku,Marcos |
dc.subject.por.fl_str_mv |
Mycobacterium bovis decontamination Petroff HPC |
topic |
Mycobacterium bovis decontamination Petroff HPC |
description |
Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822008000200008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.39 n.2 2008 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201747750912 |