In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200017 |
Resumo: | This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours post infection in comparison with parental viruses. No statistical differences were observed when plaque sizes of parental viruses or gE- viruses we analyzed separately in each cell type. However, both gE- recombinants displayed a significantly reduced plaque areas on three different cell cultures, in comparison with parental viruses, indicating that the lack of gE had the same effect on both BHV-1 subtypes, manifested by a restricted cell-to-cell spread in infected cells. |
id |
SBM-1_db29a15465277d025ee8e7ab8ef61e9d |
---|---|
oai_identifier_str |
oai:scielo:S1517-83822004000200017 |
network_acronym_str |
SBM-1 |
network_name_str |
Brazilian Journal of Microbiology |
repository_id_str |
|
spelling |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a)infectious bovine rhinotracheitis/infectious pustular vulvovaginitisdifferential vaccinein vitro growth characteristicsgE- recombinantBHV-1.2abovine herpesvirusesThis study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours post infection in comparison with parental viruses. No statistical differences were observed when plaque sizes of parental viruses or gE- viruses we analyzed separately in each cell type. However, both gE- recombinants displayed a significantly reduced plaque areas on three different cell cultures, in comparison with parental viruses, indicating that the lack of gE had the same effect on both BHV-1 subtypes, manifested by a restricted cell-to-cell spread in infected cells.Sociedade Brasileira de Microbiologia2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200017Brazilian Journal of Microbiology v.35 n.3 2004reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822004000200017info:eu-repo/semantics/openAccessSpilki,Fernando R.Franco,Ana C.Rijsewijk,Frans A. M.Weiblen,RudiFlores,Eduardo F.Roehe,Paulo M.eng2005-11-25T00:00:00Zoai:scielo:S1517-83822004000200017Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2005-11-25T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a) |
title |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a) |
spellingShingle |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a) Spilki,Fernando R. infectious bovine rhinotracheitis/infectious pustular vulvovaginitis differential vaccine in vitro growth characteristics gE- recombinant BHV-1.2a bovine herpesviruses |
title_short |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a) |
title_full |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a) |
title_fullStr |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a) |
title_full_unstemmed |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a) |
title_sort |
In vitro characterization of gE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2a (BHV-1.2a) |
author |
Spilki,Fernando R. |
author_facet |
Spilki,Fernando R. Franco,Ana C. Rijsewijk,Frans A. M. Weiblen,Rudi Flores,Eduardo F. Roehe,Paulo M. |
author_role |
author |
author2 |
Franco,Ana C. Rijsewijk,Frans A. M. Weiblen,Rudi Flores,Eduardo F. Roehe,Paulo M. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Spilki,Fernando R. Franco,Ana C. Rijsewijk,Frans A. M. Weiblen,Rudi Flores,Eduardo F. Roehe,Paulo M. |
dc.subject.por.fl_str_mv |
infectious bovine rhinotracheitis/infectious pustular vulvovaginitis differential vaccine in vitro growth characteristics gE- recombinant BHV-1.2a bovine herpesviruses |
topic |
infectious bovine rhinotracheitis/infectious pustular vulvovaginitis differential vaccine in vitro growth characteristics gE- recombinant BHV-1.2a bovine herpesviruses |
description |
This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours post infection in comparison with parental viruses. No statistical differences were observed when plaque sizes of parental viruses or gE- viruses we analyzed separately in each cell type. However, both gE- recombinants displayed a significantly reduced plaque areas on three different cell cultures, in comparison with parental viruses, indicating that the lack of gE had the same effect on both BHV-1 subtypes, manifested by a restricted cell-to-cell spread in infected cells. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822004000200017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.35 n.3 2004 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122200129798144 |