Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy

Detalhes bibliográficos
Autor(a) principal: Cui,Lunbiao
Data de Publicação: 2010
Outros Autores: Ge,Yiyue, Qi,Yuhua, Shi,Zhiyang, Jiao,Yongjun, Qi,Xian, Zhai,Xiangjun, Wang,Hua
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036
Resumo: The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection.
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spelling Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategyHBVPreS1ProteomicsGRP75The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection.Sociedade Brasileira de Microbiologia2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036Brazilian Journal of Microbiology v.41 n.2 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010000200036info:eu-repo/semantics/openAccessCui,LunbiaoGe,YiyueQi,YuhuaShi,ZhiyangJiao,YongjunQi,XianZhai,XiangjunWang,Huaeng2010-04-16T00:00:00Zoai:scielo:S1517-83822010000200036Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2010-04-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
title Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
spellingShingle Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
Cui,Lunbiao
HBV
PreS1
Proteomics
GRP75
title_short Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
title_full Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
title_fullStr Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
title_full_unstemmed Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
title_sort Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
author Cui,Lunbiao
author_facet Cui,Lunbiao
Ge,Yiyue
Qi,Yuhua
Shi,Zhiyang
Jiao,Yongjun
Qi,Xian
Zhai,Xiangjun
Wang,Hua
author_role author
author2 Ge,Yiyue
Qi,Yuhua
Shi,Zhiyang
Jiao,Yongjun
Qi,Xian
Zhai,Xiangjun
Wang,Hua
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cui,Lunbiao
Ge,Yiyue
Qi,Yuhua
Shi,Zhiyang
Jiao,Yongjun
Qi,Xian
Zhai,Xiangjun
Wang,Hua
dc.subject.por.fl_str_mv HBV
PreS1
Proteomics
GRP75
topic HBV
PreS1
Proteomics
GRP75
description The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822010000200036
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.41 n.2 2010
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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