Endo-and exoglucanase activities in bacteria from mangrove sediment
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300048 |
Resumo: | The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose. |
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Brazilian Journal of Microbiology |
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Endo-and exoglucanase activities in bacteria from mangrove sedimentcelluloseendo-1,4-β-D-glucanaseexo-1,4-β-D-glucanasesalinityThe mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose.Sociedade Brasileira de Microbiologia2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300048Brazilian Journal of Microbiology v.44 n.3 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013000300048info:eu-repo/semantics/openAccessSoares Júnior,Fábio LinoDias,Armando Cavalcante FrancoFasanella,Cristiane CipolaTaketani,Rodrigo GouvêaLima,André Oliveira de SouzaMelo,Itamar SoaresAndreote,Fernando Dinieng2014-02-03T00:00:00Zoai:scielo:S1517-83822013000300048Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-02-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Endo-and exoglucanase activities in bacteria from mangrove sediment |
title |
Endo-and exoglucanase activities in bacteria from mangrove sediment |
spellingShingle |
Endo-and exoglucanase activities in bacteria from mangrove sediment Soares Júnior,Fábio Lino cellulose endo-1,4-β-D-glucanase exo-1,4-β-D-glucanase salinity |
title_short |
Endo-and exoglucanase activities in bacteria from mangrove sediment |
title_full |
Endo-and exoglucanase activities in bacteria from mangrove sediment |
title_fullStr |
Endo-and exoglucanase activities in bacteria from mangrove sediment |
title_full_unstemmed |
Endo-and exoglucanase activities in bacteria from mangrove sediment |
title_sort |
Endo-and exoglucanase activities in bacteria from mangrove sediment |
author |
Soares Júnior,Fábio Lino |
author_facet |
Soares Júnior,Fábio Lino Dias,Armando Cavalcante Franco Fasanella,Cristiane Cipola Taketani,Rodrigo Gouvêa Lima,André Oliveira de Souza Melo,Itamar Soares Andreote,Fernando Dini |
author_role |
author |
author2 |
Dias,Armando Cavalcante Franco Fasanella,Cristiane Cipola Taketani,Rodrigo Gouvêa Lima,André Oliveira de Souza Melo,Itamar Soares Andreote,Fernando Dini |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Soares Júnior,Fábio Lino Dias,Armando Cavalcante Franco Fasanella,Cristiane Cipola Taketani,Rodrigo Gouvêa Lima,André Oliveira de Souza Melo,Itamar Soares Andreote,Fernando Dini |
dc.subject.por.fl_str_mv |
cellulose endo-1,4-β-D-glucanase exo-1,4-β-D-glucanase salinity |
topic |
cellulose endo-1,4-β-D-glucanase exo-1,4-β-D-glucanase salinity |
description |
The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300048 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300048 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822013000300048 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.44 n.3 2013 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122205616996352 |