Characterization of the Saccharomyces cerevisae Y500-4L killer toxin

Detalhes bibliográficos
Autor(a) principal: Soares,Giselle A. M.
Data de Publicação: 2000
Outros Autores: Sato,Hélia H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400010
Resumo: The strain Saccharomyces cerevisiae Y500-4L, selected from the must of alcohol producing plants, liberates a toxin which is lethal to the commercial yeast produced by Fleischmann Royal Nabisco and other strains of yeast. This toxin was characterized, and the maximum production was obtained after 24 hours of incubation at 25ºC in YEPD medium. The maximum activity was achieved between pH 4.1 and 4.5 and between 22 and 25ºC and maximum stability in the pH range 3.8 to 4.5 at -10ºC. The killer toxin was inactivated by heating at 40ºC for 1 hour at pH 4.1. After concentration by ultrafiltration of culture supernatants and purification by gel filtration chromatography, the molecular weight of the purified toxin was estimated by SDS-PAGE to be about 18-20 kDa.
id SBM-1_c60d1f3f17000196c086d4c574dc5213
oai_identifier_str oai:scielo:S1517-83822000000400010
network_acronym_str SBM-1
network_name_str Brazilian Journal of Microbiology
repository_id_str
spelling Characterization of the Saccharomyces cerevisae Y500-4L killer toxinkiller toxinSaccharomyces cerevisiaekiller yeastThe strain Saccharomyces cerevisiae Y500-4L, selected from the must of alcohol producing plants, liberates a toxin which is lethal to the commercial yeast produced by Fleischmann Royal Nabisco and other strains of yeast. This toxin was characterized, and the maximum production was obtained after 24 hours of incubation at 25ºC in YEPD medium. The maximum activity was achieved between pH 4.1 and 4.5 and between 22 and 25ºC and maximum stability in the pH range 3.8 to 4.5 at -10ºC. The killer toxin was inactivated by heating at 40ºC for 1 hour at pH 4.1. After concentration by ultrafiltration of culture supernatants and purification by gel filtration chromatography, the molecular weight of the purified toxin was estimated by SDS-PAGE to be about 18-20 kDa.Sociedade Brasileira de Microbiologia2000-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400010Brazilian Journal of Microbiology v.31 n.4 2000reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822000000400010info:eu-repo/semantics/openAccessSoares,Giselle A. M.Sato,Hélia H.eng2001-05-23T00:00:00Zoai:scielo:S1517-83822000000400010Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2001-05-23T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
title Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
spellingShingle Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
Soares,Giselle A. M.
killer toxin
Saccharomyces cerevisiae
killer yeast
title_short Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
title_full Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
title_fullStr Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
title_full_unstemmed Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
title_sort Characterization of the Saccharomyces cerevisae Y500-4L killer toxin
author Soares,Giselle A. M.
author_facet Soares,Giselle A. M.
Sato,Hélia H.
author_role author
author2 Sato,Hélia H.
author2_role author
dc.contributor.author.fl_str_mv Soares,Giselle A. M.
Sato,Hélia H.
dc.subject.por.fl_str_mv killer toxin
Saccharomyces cerevisiae
killer yeast
topic killer toxin
Saccharomyces cerevisiae
killer yeast
description The strain Saccharomyces cerevisiae Y500-4L, selected from the must of alcohol producing plants, liberates a toxin which is lethal to the commercial yeast produced by Fleischmann Royal Nabisco and other strains of yeast. This toxin was characterized, and the maximum production was obtained after 24 hours of incubation at 25ºC in YEPD medium. The maximum activity was achieved between pH 4.1 and 4.5 and between 22 and 25ºC and maximum stability in the pH range 3.8 to 4.5 at -10ºC. The killer toxin was inactivated by heating at 40ºC for 1 hour at pH 4.1. After concentration by ultrafiltration of culture supernatants and purification by gel filtration chromatography, the molecular weight of the purified toxin was estimated by SDS-PAGE to be about 18-20 kDa.
publishDate 2000
dc.date.none.fl_str_mv 2000-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822000000400010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.31 n.4 2000
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
_version_ 1752122198854729728

Registros relacionados