Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034 |
Resumo: | The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent of those samples with SCC above 200,000 (n=13) were screened for real-time PCR identification of microorganisms and blood samples were genotyped for TLR4 SNPs. There was a higher prevalence of Gram-positive bacteria in the analyzed samples (88.9%) and animals that had the combined genotypes AACCCC, GGTCGG and GACCGC presented the lowest somatic cell scores, and consequently those genotypes have the potential to be applied as molecular markers for assisted animal selection to improve milk quality. |
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Brazilian Journal of Microbiology |
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Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteinsTLR-4single nucleotide polymorphismmastitismilk qualityThe identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent of those samples with SCC above 200,000 (n=13) were screened for real-time PCR identification of microorganisms and blood samples were genotyped for TLR4 SNPs. There was a higher prevalence of Gram-positive bacteria in the analyzed samples (88.9%) and animals that had the combined genotypes AACCCC, GGTCGG and GACCGC presented the lowest somatic cell scores, and consequently those genotypes have the potential to be applied as molecular markers for assisted animal selection to improve milk quality.Sociedade Brasileira de Microbiologia2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034Brazilian Journal of Microbiology v.43 n.2 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000200034info:eu-repo/semantics/openAccessMesquita,Adriano Queiroz deRezende,Cintia Silva Minafra eMesquita,Albenones José deJardim,Eurione Antonio Garcia da VeigaKipnis,Ana Paula Junqueiraeng2012-08-07T00:00:00Zoai:scielo:S1517-83822012000200034Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-08-07T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins |
title |
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins |
spellingShingle |
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins Mesquita,Adriano Queiroz de TLR-4 single nucleotide polymorphism mastitis milk quality |
title_short |
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins |
title_full |
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins |
title_fullStr |
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins |
title_full_unstemmed |
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins |
title_sort |
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins |
author |
Mesquita,Adriano Queiroz de |
author_facet |
Mesquita,Adriano Queiroz de Rezende,Cintia Silva Minafra e Mesquita,Albenones José de Jardim,Eurione Antonio Garcia da Veiga Kipnis,Ana Paula Junqueira |
author_role |
author |
author2 |
Rezende,Cintia Silva Minafra e Mesquita,Albenones José de Jardim,Eurione Antonio Garcia da Veiga Kipnis,Ana Paula Junqueira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Mesquita,Adriano Queiroz de Rezende,Cintia Silva Minafra e Mesquita,Albenones José de Jardim,Eurione Antonio Garcia da Veiga Kipnis,Ana Paula Junqueira |
dc.subject.por.fl_str_mv |
TLR-4 single nucleotide polymorphism mastitis milk quality |
topic |
TLR-4 single nucleotide polymorphism mastitis milk quality |
description |
The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent of those samples with SCC above 200,000 (n=13) were screened for real-time PCR identification of microorganisms and blood samples were genotyped for TLR4 SNPs. There was a higher prevalence of Gram-positive bacteria in the analyzed samples (88.9%) and animals that had the combined genotypes AACCCC, GGTCGG and GACCGC presented the lowest somatic cell scores, and consequently those genotypes have the potential to be applied as molecular markers for assisted animal selection to improve milk quality. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822012000200034 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.43 n.2 2012 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122204399599616 |