Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?

Detalhes bibliográficos
Autor(a) principal: Krawczak,Felipe da Silva
Data de Publicação: 2015
Outros Autores: Reis,Ilka Afonso, Silveira,Julia Angélica da, Avelar,Daniel Moreira, Marcelino,Andreza Pain, Werneck,Guilherme Loureiro, Labruna,Marcelo Bahia, Paz,Gustavo Fontes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000100064
Resumo: INTRODUCTION: The present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-Leishmania spp., -Babesia canis vogeli and -Ehrlichia canis antibodies in urban dogs from an area endemic to these parasites. METHODS: The serum samples from dogs were tested for the Babesia canis vogeli strain Belo Horizonte antigen and Ehrlichia canis strain São Paulo by immunofluorescence antibody test (IFAT) and by anti-Leishmania immunoglobulin G (IgG) antibody detection to assess Leishmania infection. We used the following four commercial kits for canine visceral leishmaniasis: ELISA, IFAT, Dual Path Platform (DPP) (Bio Manguinhos(r)/FIOCRUZ/MS) and a rK39 RDT (Kalazar Detect Canine Rapid Test; Inbios). RESULTS : Of 96 serum samples submitted to serological assays, 4 (4.2%) were positive for Leishmania as determined by ELISA; 12 (12.5%), by IFAT; 14 (14.6%) by rK39 RDT; and 20 (20.8%), by DPP. Antibodies against Ehrlichia and Babesia were detected in 23/96 (23.9%) and 30/96 (31.2%) samples, respectively. No significant association was identified between the results of tests for detecting Babesia or Ehrlichia and those for detecting Leishmania (p-value>0.05). CONCLUSIONS: In the present study, we demonstrated co-infection with Ehrlichia or Babesia and Leishmania in dogs from Minas Gerais (Brazil); we also found that the serological tests that were used did not cross-react.
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spelling Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?Immunochromatographic testEnzyme-linked immunosorbent assayImmunofluorescence antibody testCanine visceral leishmaniasis INTRODUCTION: The present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-Leishmania spp., -Babesia canis vogeli and -Ehrlichia canis antibodies in urban dogs from an area endemic to these parasites. METHODS: The serum samples from dogs were tested for the Babesia canis vogeli strain Belo Horizonte antigen and Ehrlichia canis strain São Paulo by immunofluorescence antibody test (IFAT) and by anti-Leishmania immunoglobulin G (IgG) antibody detection to assess Leishmania infection. We used the following four commercial kits for canine visceral leishmaniasis: ELISA, IFAT, Dual Path Platform (DPP) (Bio Manguinhos(r)/FIOCRUZ/MS) and a rK39 RDT (Kalazar Detect Canine Rapid Test; Inbios). RESULTS : Of 96 serum samples submitted to serological assays, 4 (4.2%) were positive for Leishmania as determined by ELISA; 12 (12.5%), by IFAT; 14 (14.6%) by rK39 RDT; and 20 (20.8%), by DPP. Antibodies against Ehrlichia and Babesia were detected in 23/96 (23.9%) and 30/96 (31.2%) samples, respectively. No significant association was identified between the results of tests for detecting Babesia or Ehrlichia and those for detecting Leishmania (p-value>0.05). CONCLUSIONS: In the present study, we demonstrated co-infection with Ehrlichia or Babesia and Leishmania in dogs from Minas Gerais (Brazil); we also found that the serological tests that were used did not cross-react. Sociedade Brasileira de Medicina Tropical - SBMT2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000100064Revista da Sociedade Brasileira de Medicina Tropical v.48 n.1 2015reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0291-2014info:eu-repo/semantics/openAccessKrawczak,Felipe da SilvaReis,Ilka AfonsoSilveira,Julia Angélica daAvelar,Daniel MoreiraMarcelino,Andreza PainWerneck,Guilherme LoureiroLabruna,Marcelo BahiaPaz,Gustavo Fonteseng2015-03-31T00:00:00Zoai:scielo:S0037-86822015000100064Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2015-03-31T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
spellingShingle Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
Krawczak,Felipe da Silva
Immunochromatographic test
Enzyme-linked immunosorbent assay
Immunofluorescence antibody test
Canine visceral leishmaniasis
title_short Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title_full Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title_fullStr Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title_full_unstemmed Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title_sort Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
author Krawczak,Felipe da Silva
author_facet Krawczak,Felipe da Silva
Reis,Ilka Afonso
Silveira,Julia Angélica da
Avelar,Daniel Moreira
Marcelino,Andreza Pain
Werneck,Guilherme Loureiro
Labruna,Marcelo Bahia
Paz,Gustavo Fontes
author_role author
author2 Reis,Ilka Afonso
Silveira,Julia Angélica da
Avelar,Daniel Moreira
Marcelino,Andreza Pain
Werneck,Guilherme Loureiro
Labruna,Marcelo Bahia
Paz,Gustavo Fontes
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Krawczak,Felipe da Silva
Reis,Ilka Afonso
Silveira,Julia Angélica da
Avelar,Daniel Moreira
Marcelino,Andreza Pain
Werneck,Guilherme Loureiro
Labruna,Marcelo Bahia
Paz,Gustavo Fontes
dc.subject.por.fl_str_mv Immunochromatographic test
Enzyme-linked immunosorbent assay
Immunofluorescence antibody test
Canine visceral leishmaniasis
topic Immunochromatographic test
Enzyme-linked immunosorbent assay
Immunofluorescence antibody test
Canine visceral leishmaniasis
description INTRODUCTION: The present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-Leishmania spp., -Babesia canis vogeli and -Ehrlichia canis antibodies in urban dogs from an area endemic to these parasites. METHODS: The serum samples from dogs were tested for the Babesia canis vogeli strain Belo Horizonte antigen and Ehrlichia canis strain São Paulo by immunofluorescence antibody test (IFAT) and by anti-Leishmania immunoglobulin G (IgG) antibody detection to assess Leishmania infection. We used the following four commercial kits for canine visceral leishmaniasis: ELISA, IFAT, Dual Path Platform (DPP) (Bio Manguinhos(r)/FIOCRUZ/MS) and a rK39 RDT (Kalazar Detect Canine Rapid Test; Inbios). RESULTS : Of 96 serum samples submitted to serological assays, 4 (4.2%) were positive for Leishmania as determined by ELISA; 12 (12.5%), by IFAT; 14 (14.6%) by rK39 RDT; and 20 (20.8%), by DPP. Antibodies against Ehrlichia and Babesia were detected in 23/96 (23.9%) and 30/96 (31.2%) samples, respectively. No significant association was identified between the results of tests for detecting Babesia or Ehrlichia and those for detecting Leishmania (p-value>0.05). CONCLUSIONS: In the present study, we demonstrated co-infection with Ehrlichia or Babesia and Leishmania in dogs from Minas Gerais (Brazil); we also found that the serological tests that were used did not cross-react.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000100064
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000100064
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-8682-0291-2014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.48 n.1 2015
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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