Chagas' disease: an algorithm for donor screening and positive donor counseling

Detalhes bibliográficos
Autor(a) principal: Hamerschlak,Nelson
Data de Publicação: 1997
Outros Autores: Pasternak,Jacyr, Amato Neto,Vicente, Carvalho,Márcia B. de, Guerra,Carmen S., Coscina,Antonio L., Ferreira,Orlando C., Rosenblit,Jacob, Szterling,Leonel N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000300006
Resumo: Classical serological screening assays for Chagas' disease are time consuming and subjective. The objective of the present work is to evaluate the enzyme immuno-assay (ELISA) methodology and to propose an algorithm for blood banks to be applied to Chagas' disease. Seven thousand, nine hundred and ninety nine blood donor samples were screened by both reverse passive hemagglutination (RPHA) and indirect immunofluorescence assay (IFA). Samples reactive on RPHA and/or IFA were submitted to supplementary RPHA, IFA and complement fixation (CFA) tests. This strategy allowed us to create a panel of 60 samples to evaluate the ELISA methodology from 3 different manufacturers. The sensitivity of the screening by IFA and the 3 different ELISA's was 100%. The specificity was better on ELISA methodology. For Chagas disease, ELISA seems to be the best test for blood donor screening, because it showed high sensitivity and specificity, it is not subjective and can be automated. Therefore, it was possible to propose an algorithm to screen samples and confirm donor results at the blood bank.
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spelling Chagas' disease: an algorithm for donor screening and positive donor counselingChagas' diseaseChagas' serologyBlood donor screeningEnzyme immuno assay (ELISA)Classical serological screening assays for Chagas' disease are time consuming and subjective. The objective of the present work is to evaluate the enzyme immuno-assay (ELISA) methodology and to propose an algorithm for blood banks to be applied to Chagas' disease. Seven thousand, nine hundred and ninety nine blood donor samples were screened by both reverse passive hemagglutination (RPHA) and indirect immunofluorescence assay (IFA). Samples reactive on RPHA and/or IFA were submitted to supplementary RPHA, IFA and complement fixation (CFA) tests. This strategy allowed us to create a panel of 60 samples to evaluate the ELISA methodology from 3 different manufacturers. The sensitivity of the screening by IFA and the 3 different ELISA's was 100%. The specificity was better on ELISA methodology. For Chagas disease, ELISA seems to be the best test for blood donor screening, because it showed high sensitivity and specificity, it is not subjective and can be automated. Therefore, it was possible to propose an algorithm to screen samples and confirm donor results at the blood bank.Sociedade Brasileira de Medicina Tropical - SBMT1997-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000300006Revista da Sociedade Brasileira de Medicina Tropical v.30 n.3 1997reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86821997000300006info:eu-repo/semantics/openAccessHamerschlak,NelsonPasternak,JacyrAmato Neto,VicenteCarvalho,Márcia B. deGuerra,Carmen S.Coscina,Antonio L.Ferreira,Orlando C.Rosenblit,JacobSzterling,Leonel N.eng2000-06-14T00:00:00Zoai:scielo:S0037-86821997000300006Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2000-06-14T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Chagas' disease: an algorithm for donor screening and positive donor counseling
title Chagas' disease: an algorithm for donor screening and positive donor counseling
spellingShingle Chagas' disease: an algorithm for donor screening and positive donor counseling
Hamerschlak,Nelson
Chagas' disease
Chagas' serology
Blood donor screening
Enzyme immuno assay (ELISA)
title_short Chagas' disease: an algorithm for donor screening and positive donor counseling
title_full Chagas' disease: an algorithm for donor screening and positive donor counseling
title_fullStr Chagas' disease: an algorithm for donor screening and positive donor counseling
title_full_unstemmed Chagas' disease: an algorithm for donor screening and positive donor counseling
title_sort Chagas' disease: an algorithm for donor screening and positive donor counseling
author Hamerschlak,Nelson
author_facet Hamerschlak,Nelson
Pasternak,Jacyr
Amato Neto,Vicente
Carvalho,Márcia B. de
Guerra,Carmen S.
Coscina,Antonio L.
Ferreira,Orlando C.
Rosenblit,Jacob
Szterling,Leonel N.
author_role author
author2 Pasternak,Jacyr
Amato Neto,Vicente
Carvalho,Márcia B. de
Guerra,Carmen S.
Coscina,Antonio L.
Ferreira,Orlando C.
Rosenblit,Jacob
Szterling,Leonel N.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hamerschlak,Nelson
Pasternak,Jacyr
Amato Neto,Vicente
Carvalho,Márcia B. de
Guerra,Carmen S.
Coscina,Antonio L.
Ferreira,Orlando C.
Rosenblit,Jacob
Szterling,Leonel N.
dc.subject.por.fl_str_mv Chagas' disease
Chagas' serology
Blood donor screening
Enzyme immuno assay (ELISA)
topic Chagas' disease
Chagas' serology
Blood donor screening
Enzyme immuno assay (ELISA)
description Classical serological screening assays for Chagas' disease are time consuming and subjective. The objective of the present work is to evaluate the enzyme immuno-assay (ELISA) methodology and to propose an algorithm for blood banks to be applied to Chagas' disease. Seven thousand, nine hundred and ninety nine blood donor samples were screened by both reverse passive hemagglutination (RPHA) and indirect immunofluorescence assay (IFA). Samples reactive on RPHA and/or IFA were submitted to supplementary RPHA, IFA and complement fixation (CFA) tests. This strategy allowed us to create a panel of 60 samples to evaluate the ELISA methodology from 3 different manufacturers. The sensitivity of the screening by IFA and the 3 different ELISA's was 100%. The specificity was better on ELISA methodology. For Chagas disease, ELISA seems to be the best test for blood donor screening, because it showed high sensitivity and specificity, it is not subjective and can be automated. Therefore, it was possible to propose an algorithm to screen samples and confirm donor results at the blood bank.
publishDate 1997
dc.date.none.fl_str_mv 1997-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000300006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000300006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86821997000300006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.30 n.3 1997
reponame:Revista da Sociedade Brasileira de Medicina Tropical
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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