Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection

Detalhes bibliográficos
Autor(a) principal: Gilber,Soraia Reda
Data de Publicação: 2013
Outros Autores: Alban,Silvana Maria, Gobor,Luiza, Bescrovaine,Jessica de Oliveira, Myiazaki,Marcia Iurico, Thomaz-Soccol,Vanete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300310
Resumo: Introduction Trypanosoma cruzi, a flagellated protozoan, is the etiologic agent of Chagas disease, and it is estimated that approximately 5 million people in Brazil are infected with this parasite. This work aimed to compare the current diagnostic methods for Chagas disease, including conventional serological (IFAT and ELISA) and molecular techniques (PCR), to introduce PCR as an auxiliary technique. Methods A total of 106 chagasic patients were evaluated: 88 from endemic areas of Parana, 6 from São Paulo, 3 from Minas Gerais, 3 from Rio Grande do Sul, 1 from Bahia and 5 from the Santa Catarina T. cruzi outbreak. The samples were analyzed by conventional serological methods (IFAT, ELISA), hemoculture and PCR to confirm Chagas disease. Results When IFAT was used to determine antibody levels, the sensitivity was 81.7% for patients with the cardiac form of the disease and 100% for the other clinical forms. In contrast, ELISA showed 84% sensitivity and 100% specificity. The use of serological and molecular techniques and their implications for the diagnosis of Chagas disease in non-endemics area are discussed. Conclusions PCR constitutes an excellent support methodology for the laboratory diagnosis of Chagas disease due to its high sensitivity and specificity.
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spelling Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infectionChagas diseaseInconclusive diagnosisRoutine diagnosis Introduction Trypanosoma cruzi, a flagellated protozoan, is the etiologic agent of Chagas disease, and it is estimated that approximately 5 million people in Brazil are infected with this parasite. This work aimed to compare the current diagnostic methods for Chagas disease, including conventional serological (IFAT and ELISA) and molecular techniques (PCR), to introduce PCR as an auxiliary technique. Methods A total of 106 chagasic patients were evaluated: 88 from endemic areas of Parana, 6 from São Paulo, 3 from Minas Gerais, 3 from Rio Grande do Sul, 1 from Bahia and 5 from the Santa Catarina T. cruzi outbreak. The samples were analyzed by conventional serological methods (IFAT, ELISA), hemoculture and PCR to confirm Chagas disease. Results When IFAT was used to determine antibody levels, the sensitivity was 81.7% for patients with the cardiac form of the disease and 100% for the other clinical forms. In contrast, ELISA showed 84% sensitivity and 100% specificity. The use of serological and molecular techniques and their implications for the diagnosis of Chagas disease in non-endemics area are discussed. Conclusions PCR constitutes an excellent support methodology for the laboratory diagnosis of Chagas disease due to its high sensitivity and specificity. Sociedade Brasileira de Medicina Tropical - SBMT2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300310Revista da Sociedade Brasileira de Medicina Tropical v.46 n.3 2013reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0046-2013info:eu-repo/semantics/openAccessGilber,Soraia RedaAlban,Silvana MariaGobor,LuizaBescrovaine,Jessica de OliveiraMyiazaki,Marcia IuricoThomaz-Soccol,Vaneteeng2013-07-05T00:00:00Zoai:scielo:S0037-86822013000300310Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2013-07-05T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection
title Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection
spellingShingle Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection
Gilber,Soraia Reda
Chagas disease
Inconclusive diagnosis
Routine diagnosis
title_short Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection
title_full Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection
title_fullStr Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection
title_full_unstemmed Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection
title_sort Comparison of conventional serology and PCR methods for the routine diagnosis of Trypanosoma cruzi infection
author Gilber,Soraia Reda
author_facet Gilber,Soraia Reda
Alban,Silvana Maria
Gobor,Luiza
Bescrovaine,Jessica de Oliveira
Myiazaki,Marcia Iurico
Thomaz-Soccol,Vanete
author_role author
author2 Alban,Silvana Maria
Gobor,Luiza
Bescrovaine,Jessica de Oliveira
Myiazaki,Marcia Iurico
Thomaz-Soccol,Vanete
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gilber,Soraia Reda
Alban,Silvana Maria
Gobor,Luiza
Bescrovaine,Jessica de Oliveira
Myiazaki,Marcia Iurico
Thomaz-Soccol,Vanete
dc.subject.por.fl_str_mv Chagas disease
Inconclusive diagnosis
Routine diagnosis
topic Chagas disease
Inconclusive diagnosis
Routine diagnosis
description Introduction Trypanosoma cruzi, a flagellated protozoan, is the etiologic agent of Chagas disease, and it is estimated that approximately 5 million people in Brazil are infected with this parasite. This work aimed to compare the current diagnostic methods for Chagas disease, including conventional serological (IFAT and ELISA) and molecular techniques (PCR), to introduce PCR as an auxiliary technique. Methods A total of 106 chagasic patients were evaluated: 88 from endemic areas of Parana, 6 from São Paulo, 3 from Minas Gerais, 3 from Rio Grande do Sul, 1 from Bahia and 5 from the Santa Catarina T. cruzi outbreak. The samples were analyzed by conventional serological methods (IFAT, ELISA), hemoculture and PCR to confirm Chagas disease. Results When IFAT was used to determine antibody levels, the sensitivity was 81.7% for patients with the cardiac form of the disease and 100% for the other clinical forms. In contrast, ELISA showed 84% sensitivity and 100% specificity. The use of serological and molecular techniques and their implications for the diagnosis of Chagas disease in non-endemics area are discussed. Conclusions PCR constitutes an excellent support methodology for the laboratory diagnosis of Chagas disease due to its high sensitivity and specificity.
publishDate 2013
dc.date.none.fl_str_mv 2013-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000300310
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/0037-8682-0046-2013
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.46 n.3 2013
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repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
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