Experimento didático sobre cromatografia gasosa: uma abordagem analítica e ambiental

• Autor(a) principal: Penteado,José Carlos P.
• Data de Publicação: 2008
• Outros Autores: Magalhães,Dulce, Masini,Jorge C.
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000800047
• topic: benzene; internal standard; contamination

This paper describes an experiment to teach the principles of gas chromatography exploring the boiling points and polarities to explain the elution order of a series of alcohols, benzene and n-propanone, as well as to teach the response factor concept and the internal standard addition method. Retention times and response factors are used for qualitative identification and quantitative analysis of a hypothetical contamination source in a simulated water sample. The internal standard n-propanol is further used for quantification of benzene and n-butanol in the water sample. This experiment has been taught in the instrumental analysis course offered to chemistry and oceanography students.