An evaluation of antibacterial activities of Psidium guajava (L.)
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300014 |
Resumo: | The present study was designated to evaluate the antibacterial activities of aqueous and ethanol:water extracts from leaves, roots and stem bark of Psidium guajava L. The antibacterial activities of the extracts against bacteria were tested by using both microdilution assay. The aqueous extracts of P. guajava leaves, roots and stem bark were active against the gram-positive bacteria Staphylococcus aureus (MICs=500, 125 and 250 µg/ml, respectively) and Bacillus subtilis (MICs=500 µg/ml), and virtually inactive against the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (MICs >1000 µg/ml). The ethanol:water extracts showed higher antimicrobial activity as compared to aqueous extracts. Based on this finding, the ethanol:water extract of P. guajava leaves was fractionated on silica gel column chromatography in a bioassay-guided fractionation affording flavonoid mixture, triterpenes (alpha- and beta-amyrin) and sterol (beta-sitosterol). Flavonoid mixture showed good activity on S. aureus with MIC of 25 µg/ml. beta-sitosterol was inactive for all the bacteria tested. |
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An evaluation of antibacterial activities of Psidium guajava (L.)Psidium guajavaantibacterial activitiesflavonoidsalpha- and beta-amyrinbeta-sitosterolThe present study was designated to evaluate the antibacterial activities of aqueous and ethanol:water extracts from leaves, roots and stem bark of Psidium guajava L. The antibacterial activities of the extracts against bacteria were tested by using both microdilution assay. The aqueous extracts of P. guajava leaves, roots and stem bark were active against the gram-positive bacteria Staphylococcus aureus (MICs=500, 125 and 250 µg/ml, respectively) and Bacillus subtilis (MICs=500 µg/ml), and virtually inactive against the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (MICs >1000 µg/ml). The ethanol:water extracts showed higher antimicrobial activity as compared to aqueous extracts. Based on this finding, the ethanol:water extract of P. guajava leaves was fractionated on silica gel column chromatography in a bioassay-guided fractionation affording flavonoid mixture, triterpenes (alpha- and beta-amyrin) and sterol (beta-sitosterol). Flavonoid mixture showed good activity on S. aureus with MIC of 25 µg/ml. beta-sitosterol was inactive for all the bacteria tested.Instituto de Tecnologia do Paraná - Tecpar2005-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300014Brazilian Archives of Biology and Technology v.48 n.3 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000300014info:eu-repo/semantics/openAccessSanches,Neviton RogérioGarcia Cortez,Diógenes AparícioSchiavini,Michelle SimoneNakamura,Celso VataruDias Filho,Benedito Pradoeng2005-08-03T00:00:00Zoai:scielo:S1516-89132005000300014Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-08-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
An evaluation of antibacterial activities of Psidium guajava (L.) |
title |
An evaluation of antibacterial activities of Psidium guajava (L.) |
spellingShingle |
An evaluation of antibacterial activities of Psidium guajava (L.) Sanches,Neviton Rogério Psidium guajava antibacterial activities flavonoids alpha- and beta-amyrin beta-sitosterol |
title_short |
An evaluation of antibacterial activities of Psidium guajava (L.) |
title_full |
An evaluation of antibacterial activities of Psidium guajava (L.) |
title_fullStr |
An evaluation of antibacterial activities of Psidium guajava (L.) |
title_full_unstemmed |
An evaluation of antibacterial activities of Psidium guajava (L.) |
title_sort |
An evaluation of antibacterial activities of Psidium guajava (L.) |
author |
Sanches,Neviton Rogério |
author_facet |
Sanches,Neviton Rogério Garcia Cortez,Diógenes Aparício Schiavini,Michelle Simone Nakamura,Celso Vataru Dias Filho,Benedito Prado |
author_role |
author |
author2 |
Garcia Cortez,Diógenes Aparício Schiavini,Michelle Simone Nakamura,Celso Vataru Dias Filho,Benedito Prado |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Sanches,Neviton Rogério Garcia Cortez,Diógenes Aparício Schiavini,Michelle Simone Nakamura,Celso Vataru Dias Filho,Benedito Prado |
dc.subject.por.fl_str_mv |
Psidium guajava antibacterial activities flavonoids alpha- and beta-amyrin beta-sitosterol |
topic |
Psidium guajava antibacterial activities flavonoids alpha- and beta-amyrin beta-sitosterol |
description |
The present study was designated to evaluate the antibacterial activities of aqueous and ethanol:water extracts from leaves, roots and stem bark of Psidium guajava L. The antibacterial activities of the extracts against bacteria were tested by using both microdilution assay. The aqueous extracts of P. guajava leaves, roots and stem bark were active against the gram-positive bacteria Staphylococcus aureus (MICs=500, 125 and 250 µg/ml, respectively) and Bacillus subtilis (MICs=500 µg/ml), and virtually inactive against the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (MICs >1000 µg/ml). The ethanol:water extracts showed higher antimicrobial activity as compared to aqueous extracts. Based on this finding, the ethanol:water extract of P. guajava leaves was fractionated on silica gel column chromatography in a bioassay-guided fractionation affording flavonoid mixture, triterpenes (alpha- and beta-amyrin) and sterol (beta-sitosterol). Flavonoid mixture showed good activity on S. aureus with MIC of 25 µg/ml. beta-sitosterol was inactive for all the bacteria tested. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132005000300014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.48 n.3 2005 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318270278270976 |