Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares

Detalhes bibliográficos
Autor(a) principal: Modesto, Karina Ribeiro
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UCB
Texto Completo: https://bdtd.ucb.br:8443/jspui/handle/tede/2270
Resumo: Recent discoveries points towards seminal plasma playing a major role in directing processes on mammalian spermatozoa as well as influencing offspring development prior to and after birth. Amongst the candidates for such role, recent data has brought the extracellular vesicles (EVs) into the field. Aiming to characterize EVs present on the intraluminal fluid of different segments of the epididymis, such as cauda and caput in male Wistar rats, this research assessed the influence of sexual developmental stages on the concentration and size distribution of epididymal EVs. The individuals were also grouped in terms of sexual developmental stages, clustered as ???prior to puberty, ???puberty???, and ???post-puberty???. TRPS based analysis showed a significant shifts in concentration and diameter of such EVs between clustering groups as well as epididymal segment groups. Caput epididymal EVs were found presenting reduced concentration and reduced diameter when compared to cauda epididymis EVs. Regarding the more sexually developed individuals, those showed higher EVs counts than ???prior to puberty??? ones. Additional findings suggest that post-puberty epididymal EVs are enlarged in comparison to the two other groups. Such data might serve a purpose of better comprehending the processes regarding spermatic maturation and capacitation (fertilization), offering findings that bring up clinically important issues regarding male epigenesis and father-to-offspring effects.
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spelling Pereira, Rinaldo Wellersonhttp://lattes.cnpq.br/9065501029560884http://lattes.cnpq.br/7057713890623927Modesto, Karina Ribeiro2017-09-08T11:57:33Z2017-03-31MODESTO, Karina Ribeiro. Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares. 2017. 82 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2017.https://bdtd.ucb.br:8443/jspui/handle/tede/2270Recent discoveries points towards seminal plasma playing a major role in directing processes on mammalian spermatozoa as well as influencing offspring development prior to and after birth. Amongst the candidates for such role, recent data has brought the extracellular vesicles (EVs) into the field. Aiming to characterize EVs present on the intraluminal fluid of different segments of the epididymis, such as cauda and caput in male Wistar rats, this research assessed the influence of sexual developmental stages on the concentration and size distribution of epididymal EVs. The individuals were also grouped in terms of sexual developmental stages, clustered as ???prior to puberty, ???puberty???, and ???post-puberty???. TRPS based analysis showed a significant shifts in concentration and diameter of such EVs between clustering groups as well as epididymal segment groups. Caput epididymal EVs were found presenting reduced concentration and reduced diameter when compared to cauda epididymis EVs. Regarding the more sexually developed individuals, those showed higher EVs counts than ???prior to puberty??? ones. Additional findings suggest that post-puberty epididymal EVs are enlarged in comparison to the two other groups. Such data might serve a purpose of better comprehending the processes regarding spermatic maturation and capacitation (fertilization), offering findings that bring up clinically important issues regarding male epigenesis and father-to-offspring effects.Evid??ncias crescentes t??m apontado para a import??ncia do conte??do presente no plasma seminal de mam??feros sobre as c??lulas esperm??ticas bem como sobre o desenvolvimento da prole durante os per??odos pr?? e p??s-natal. Entre os mediadores aparentemente envolvidos nesse processo, podemos citar as ves??culas extracelulares (VEs). Com o objetivo de caracterizar as VEs presentes no l??quido intraluminal de diferentes compartimentos do epid??dimo, cabe??a e cauda, de ratos machos da linhagem Wistar, o presente trabalho avaliou a influ??ncia da idade sobre a concentra????o e a distribui????o de tamanho dessas ves??culas nos compartimentos do epid??dimo. Para isso, utilizou-se tr??s grupos de ratos em idades distintas, sendo um grupo com indiv??duos em idade pr??-p??bere, um grupo com indiv??duos em idade p??bere e um grupo com indiv??duos em idade p??s-p??bere. A contagem de part??culas por detec????o de pulso resistivo ajust??vel (TRPS) revelou diferen??as importantes na concentra????o e no di??metro dessas VEs, tanto em fun????o do segmento anat??mico epididim??rio, quanto em fun????o da idade dos indiv??duos. A cabe??a do epid??dimo apresentou menor concentra????o de part??culas em compara????o com a cauda, bem como part??culas com di??metro menor. Com rela????o a idade, os ratos mais velhos apresentaram maior concentra????o de VEs quando comparados com os ratos imaturos sexualmente. Al??m disso, foi observado que as VEs presentes no ducto epididim??rio dos ratos p??s-p??beres possui tamanho maior que as VEs dos outros dois grupos experimentais. Os resultados aqui obtidos podem servir para uma melhor compreens??o dos processos que englobam tanto a matura????o e capacita????o esperm??tica em si, oferecendo propriedades clinicamente relevantes, quanto processos da espermatog??nese masculina e poss??veis efeitos epigen??ticos transgeracionais.Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-09-08T11:57:20Z No. of bitstreams: 1 KarinaRibeiroModestoDissertacao2017.pdf: 6705375 bytes, checksum: 161944cc6fbeb260f63db5fea9e61056 (MD5)Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-09-08T11:57:33Z (GMT) No. of bitstreams: 1 KarinaRibeiroModestoDissertacao2017.pdf: 6705375 bytes, checksum: 161944cc6fbeb260f63db5fea9e61056 (MD5)Made available in DSpace on 2017-09-08T11:57:33Z (GMT). No. of bitstreams: 1 KarinaRibeiroModestoDissertacao2017.pdf: 6705375 bytes, checksum: 161944cc6fbeb260f63db5fea9e61056 (MD5) Previous issue date: 2017-03-31application/pdfhttps://bdtd.ucb.br:8443/jspui/retrieve/5119/KarinaRibeiroModestoDissertacao2017.pdf.jpgporUniversidade Cat??lica de Bras??liaPrograma Strictu Sensu em Ci??ncias Gen??micas e BiotecnologiaUCBBrasilEscola de Sa??de e MedicinaEpigen??ticaProstassomoEspermatozoideEpididimossomoL??quido epididim??rioVes??cula extracelularEpigen??tica transgeracionalCNPQ::CIENCIAS BIOLOGICAS::GENETICAImpacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelularesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UCBinstname:Universidade Católica de Brasília (UCB)instacron:UCBLICENSElicense.txtlicense.txttext/plain; charset=utf-82122https://200.214.135.178:8443/jspui/bitstream/tede/2270/1/license.txt302d2cd6169132532f8ce4ab3974cba3MD51ORIGINALKarinaRibeiroModestoDissertacao2017.pdfKarinaRibeiroModestoDissertacao2017.pdfapplication/pdf6705375https://200.214.135.178:8443/jspui/bitstream/tede/2270/2/KarinaRibeiroModestoDissertacao2017.pdf161944cc6fbeb260f63db5fea9e61056MD52TEXTKarinaRibeiroModestoDissertacao2017.pdf.txtKarinaRibeiroModestoDissertacao2017.pdf.txttext/plain153024https://200.214.135.178:8443/jspui/bitstream/tede/2270/3/KarinaRibeiroModestoDissertacao2017.pdf.txtd226bc807a851bb4af01caf3c8c59df2MD53THUMBNAILKarinaRibeiroModestoDissertacao2017.pdf.jpgKarinaRibeiroModestoDissertacao2017.pdf.jpgimage/jpeg6051https://200.214.135.178:8443/jspui/bitstream/tede/2270/4/KarinaRibeiroModestoDissertacao2017.pdf.jpg237d46099fd213e8f0ad883b18ad2c9aMD54tede/22702019-09-10 11:41:13.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Biblioteca Digital de Teses e Dissertaçõeshttps://bdtd.ucb.br:8443/jspui/
dc.title.por.fl_str_mv Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares
title Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares
spellingShingle Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares
Modesto, Karina Ribeiro
Epigen??tica
Prostassomo
Espermatozoide
Epididimossomo
L??quido epididim??rio
Ves??cula extracelular
Epigen??tica transgeracional
CNPQ::CIENCIAS BIOLOGICAS::GENETICA
title_short Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares
title_full Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares
title_fullStr Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares
title_full_unstemmed Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares
title_sort Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares
author Modesto, Karina Ribeiro
author_facet Modesto, Karina Ribeiro
author_role author
dc.contributor.advisor1.fl_str_mv Pereira, Rinaldo Wellerson
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9065501029560884
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7057713890623927
dc.contributor.author.fl_str_mv Modesto, Karina Ribeiro
contributor_str_mv Pereira, Rinaldo Wellerson
dc.subject.por.fl_str_mv Epigen??tica
Prostassomo
Espermatozoide
Epididimossomo
L??quido epididim??rio
Ves??cula extracelular
Epigen??tica transgeracional
topic Epigen??tica
Prostassomo
Espermatozoide
Epididimossomo
L??quido epididim??rio
Ves??cula extracelular
Epigen??tica transgeracional
CNPQ::CIENCIAS BIOLOGICAS::GENETICA
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::GENETICA
dc.description.abstract.eng.fl_txt_mv Recent discoveries points towards seminal plasma playing a major role in directing processes on mammalian spermatozoa as well as influencing offspring development prior to and after birth. Amongst the candidates for such role, recent data has brought the extracellular vesicles (EVs) into the field. Aiming to characterize EVs present on the intraluminal fluid of different segments of the epididymis, such as cauda and caput in male Wistar rats, this research assessed the influence of sexual developmental stages on the concentration and size distribution of epididymal EVs. The individuals were also grouped in terms of sexual developmental stages, clustered as ???prior to puberty, ???puberty???, and ???post-puberty???. TRPS based analysis showed a significant shifts in concentration and diameter of such EVs between clustering groups as well as epididymal segment groups. Caput epididymal EVs were found presenting reduced concentration and reduced diameter when compared to cauda epididymis EVs. Regarding the more sexually developed individuals, those showed higher EVs counts than ???prior to puberty??? ones. Additional findings suggest that post-puberty epididymal EVs are enlarged in comparison to the two other groups. Such data might serve a purpose of better comprehending the processes regarding spermatic maturation and capacitation (fertilization), offering findings that bring up clinically important issues regarding male epigenesis and father-to-offspring effects.
dc.description.abstract.por.fl_txt_mv Evid??ncias crescentes t??m apontado para a import??ncia do conte??do presente no plasma seminal de mam??feros sobre as c??lulas esperm??ticas bem como sobre o desenvolvimento da prole durante os per??odos pr?? e p??s-natal. Entre os mediadores aparentemente envolvidos nesse processo, podemos citar as ves??culas extracelulares (VEs). Com o objetivo de caracterizar as VEs presentes no l??quido intraluminal de diferentes compartimentos do epid??dimo, cabe??a e cauda, de ratos machos da linhagem Wistar, o presente trabalho avaliou a influ??ncia da idade sobre a concentra????o e a distribui????o de tamanho dessas ves??culas nos compartimentos do epid??dimo. Para isso, utilizou-se tr??s grupos de ratos em idades distintas, sendo um grupo com indiv??duos em idade pr??-p??bere, um grupo com indiv??duos em idade p??bere e um grupo com indiv??duos em idade p??s-p??bere. A contagem de part??culas por detec????o de pulso resistivo ajust??vel (TRPS) revelou diferen??as importantes na concentra????o e no di??metro dessas VEs, tanto em fun????o do segmento anat??mico epididim??rio, quanto em fun????o da idade dos indiv??duos. A cabe??a do epid??dimo apresentou menor concentra????o de part??culas em compara????o com a cauda, bem como part??culas com di??metro menor. Com rela????o a idade, os ratos mais velhos apresentaram maior concentra????o de VEs quando comparados com os ratos imaturos sexualmente. Al??m disso, foi observado que as VEs presentes no ducto epididim??rio dos ratos p??s-p??beres possui tamanho maior que as VEs dos outros dois grupos experimentais. Os resultados aqui obtidos podem servir para uma melhor compreens??o dos processos que englobam tanto a matura????o e capacita????o esperm??tica em si, oferecendo propriedades clinicamente relevantes, quanto processos da espermatog??nese masculina e poss??veis efeitos epigen??ticos transgeracionais.
description Recent discoveries points towards seminal plasma playing a major role in directing processes on mammalian spermatozoa as well as influencing offspring development prior to and after birth. Amongst the candidates for such role, recent data has brought the extracellular vesicles (EVs) into the field. Aiming to characterize EVs present on the intraluminal fluid of different segments of the epididymis, such as cauda and caput in male Wistar rats, this research assessed the influence of sexual developmental stages on the concentration and size distribution of epididymal EVs. The individuals were also grouped in terms of sexual developmental stages, clustered as ???prior to puberty, ???puberty???, and ???post-puberty???. TRPS based analysis showed a significant shifts in concentration and diameter of such EVs between clustering groups as well as epididymal segment groups. Caput epididymal EVs were found presenting reduced concentration and reduced diameter when compared to cauda epididymis EVs. Regarding the more sexually developed individuals, those showed higher EVs counts than ???prior to puberty??? ones. Additional findings suggest that post-puberty epididymal EVs are enlarged in comparison to the two other groups. Such data might serve a purpose of better comprehending the processes regarding spermatic maturation and capacitation (fertilization), offering findings that bring up clinically important issues regarding male epigenesis and father-to-offspring effects.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-09-08T11:57:33Z
dc.date.issued.fl_str_mv 2017-03-31
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dc.identifier.citation.fl_str_mv MODESTO, Karina Ribeiro. Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares. 2017. 82 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2017.
dc.identifier.uri.fl_str_mv https://bdtd.ucb.br:8443/jspui/handle/tede/2270
identifier_str_mv MODESTO, Karina Ribeiro. Impacto da idade e do segmento epididim??rio na concentra????o e na distribui????o de tamanho de ves??culas extracelulares. 2017. 82 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, Bras??lia, 2017.
url https://bdtd.ucb.br:8443/jspui/handle/tede/2270
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dc.publisher.none.fl_str_mv Universidade Cat??lica de Bras??lia
dc.publisher.program.fl_str_mv Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia
dc.publisher.initials.fl_str_mv UCB
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Sa??de e Medicina
publisher.none.fl_str_mv Universidade Cat??lica de Bras??lia
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UCB
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