Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35377 |
Resumo: | Leptospirosis is a zoonosis of worldwide distribution in which the agent can infect several animal species and accidentally humans. In view of the limited number of studies on anti-Leptospira antibodies in wild animal species, especially those living in aquatic environments, we sought in this study to investigate the presence of these antibodies in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity in the Rodrigues Alves Botanical Garden–Amazon Zoobotanical Park, located in Belém, Pará State, Brazil. Serum samples were collected from 31 turtles, and identification of anti-Leptospira antibodies was performed using the microscopic agglutination test, using a collection of 31 live antigens which represent 19 serogroups of Leptospira. Among the analyzed samples, 54.83% (17/31) were observed to be reactive, and co-agglutination was detected in a further six samples which were not accounted for in the frequency of serogroups. The most frequently detected serogroups were Tarassovi 72.72% (8/11), Celledoni 18.18% (2/11), and Pomona 9.09% (1/11)], with titers ranging from 200 to 400, being this the first study to report infection of these serogroups in this species of chelonios. The animals were kept in water tanks, which probably favored the transport of the agent and allowed its transmission to the spot-legged turtle. We thus confirmed presence of anti-Leptospira antibodies in chelonians maintained in the Rodrigues Alves Botanical Garden. |
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Semina. Ciências Agrárias (Online) |
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Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivityIdentificação de anticorpos para Leptospira spp. em aperemas (Rhinoclemmys punctularia) mantidas em cativeiroLeptospirosisMicroscopic agglutination testWild animal.LeptospiroseTeste de aglutinação microscópicaAnimal selvagem.Leptospirosis is a zoonosis of worldwide distribution in which the agent can infect several animal species and accidentally humans. In view of the limited number of studies on anti-Leptospira antibodies in wild animal species, especially those living in aquatic environments, we sought in this study to investigate the presence of these antibodies in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity in the Rodrigues Alves Botanical Garden–Amazon Zoobotanical Park, located in Belém, Pará State, Brazil. Serum samples were collected from 31 turtles, and identification of anti-Leptospira antibodies was performed using the microscopic agglutination test, using a collection of 31 live antigens which represent 19 serogroups of Leptospira. Among the analyzed samples, 54.83% (17/31) were observed to be reactive, and co-agglutination was detected in a further six samples which were not accounted for in the frequency of serogroups. The most frequently detected serogroups were Tarassovi 72.72% (8/11), Celledoni 18.18% (2/11), and Pomona 9.09% (1/11)], with titers ranging from 200 to 400, being this the first study to report infection of these serogroups in this species of chelonios. The animals were kept in water tanks, which probably favored the transport of the agent and allowed its transmission to the spot-legged turtle. We thus confirmed presence of anti-Leptospira antibodies in chelonians maintained in the Rodrigues Alves Botanical Garden.A leptospirose é uma zoonose de distribuição mundial, na qual o agente pode infectar várias espécies animais e acidentalmente, humanos. Tendo em vista o número limitado de estudos sobre anticorpos anti-Leptospira em espécies de animais silvestres, especialmente aqueles que vivem em ambientes aquáticos, buscou-se neste estudo investigar a presença desses anticorpos em aperemas (Rhinoclemmys punctularia) mantidas em cativeiro no Jardim Botânico Rodrigues Alves - Parque Zoobotânico da Amazônia, localizado em Belém, estado do Pará, Brasil. Amostras de soro foram coletadas de 31 tartarugas e a identificação de anticorpos anti-Leptospira foi realizada pelo teste de aglutinação microscópica, utilizando-se uma coleção de 31 antígenos vivos que representam 19 sorogrupos de Leptospira. Entre as amostras analisadas, observou-se que 54,83% (17/31) foram reativas, e detectou-se co-aglutinação em mais seis amostras que não foram contabilizadas na frequência de sorogrupos. Os sorogrupos mais frequentemente detectados foram Tarassovi 72,72% (8/11), Celledoni 18,18% (2/11) e Pomona 9,09% (1/11), com títulos variando de 200 a 400, sendo este o primeiro estudo a relatar a detecção de anticorpos anti-Leptospira spp. nesta espécie de quelônio. Os animais eram mantidos em tanques de água, o que provavelmente favoreceu o transporte do agente e permitindo sua transmissão para as aperemas. Assim, confirmou-se a presença de anticorpos anti-Leptospira em quelônios mantidos no Jardim Botânico Rodrigues Alves.UEL2019-10-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3537710.5433/1679-0359.2019v40n6Supl3p3763Semina: Ciências Agrárias; Vol. 40 No. 6Supl3 (2019); 3763-3768Semina: Ciências Agrárias; v. 40 n. 6Supl3 (2019); 3763-37681679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35377/26256Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessRocha, Katarine de SouzaBaia, Ianny Watuzy MonteiroMonteiro, Louysse HeleneMiranda, Juliana Maria SantosMonteiro, Thamillys Rayssa MarquesSilva, Andréia Ferreira daReis, Thalita Amaral dosFerreira, Maeli Fernanda SilvaMesquita, Ellen Yasmin EguchiMoraes, Carla Cristina Guimarães de2022-10-10T15:41:51Zoai:ojs.pkp.sfu.ca:article/35377Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T15:41:51Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity Identificação de anticorpos para Leptospira spp. em aperemas (Rhinoclemmys punctularia) mantidas em cativeiro |
title |
Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity |
spellingShingle |
Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity Rocha, Katarine de Souza Leptospirosis Microscopic agglutination test Wild animal. Leptospirose Teste de aglutinação microscópica Animal selvagem. |
title_short |
Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity |
title_full |
Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity |
title_fullStr |
Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity |
title_full_unstemmed |
Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity |
title_sort |
Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity |
author |
Rocha, Katarine de Souza |
author_facet |
Rocha, Katarine de Souza Baia, Ianny Watuzy Monteiro Monteiro, Louysse Helene Miranda, Juliana Maria Santos Monteiro, Thamillys Rayssa Marques Silva, Andréia Ferreira da Reis, Thalita Amaral dos Ferreira, Maeli Fernanda Silva Mesquita, Ellen Yasmin Eguchi Moraes, Carla Cristina Guimarães de |
author_role |
author |
author2 |
Baia, Ianny Watuzy Monteiro Monteiro, Louysse Helene Miranda, Juliana Maria Santos Monteiro, Thamillys Rayssa Marques Silva, Andréia Ferreira da Reis, Thalita Amaral dos Ferreira, Maeli Fernanda Silva Mesquita, Ellen Yasmin Eguchi Moraes, Carla Cristina Guimarães de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rocha, Katarine de Souza Baia, Ianny Watuzy Monteiro Monteiro, Louysse Helene Miranda, Juliana Maria Santos Monteiro, Thamillys Rayssa Marques Silva, Andréia Ferreira da Reis, Thalita Amaral dos Ferreira, Maeli Fernanda Silva Mesquita, Ellen Yasmin Eguchi Moraes, Carla Cristina Guimarães de |
dc.subject.por.fl_str_mv |
Leptospirosis Microscopic agglutination test Wild animal. Leptospirose Teste de aglutinação microscópica Animal selvagem. |
topic |
Leptospirosis Microscopic agglutination test Wild animal. Leptospirose Teste de aglutinação microscópica Animal selvagem. |
description |
Leptospirosis is a zoonosis of worldwide distribution in which the agent can infect several animal species and accidentally humans. In view of the limited number of studies on anti-Leptospira antibodies in wild animal species, especially those living in aquatic environments, we sought in this study to investigate the presence of these antibodies in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity in the Rodrigues Alves Botanical Garden–Amazon Zoobotanical Park, located in Belém, Pará State, Brazil. Serum samples were collected from 31 turtles, and identification of anti-Leptospira antibodies was performed using the microscopic agglutination test, using a collection of 31 live antigens which represent 19 serogroups of Leptospira. Among the analyzed samples, 54.83% (17/31) were observed to be reactive, and co-agglutination was detected in a further six samples which were not accounted for in the frequency of serogroups. The most frequently detected serogroups were Tarassovi 72.72% (8/11), Celledoni 18.18% (2/11), and Pomona 9.09% (1/11)], with titers ranging from 200 to 400, being this the first study to report infection of these serogroups in this species of chelonios. The animals were kept in water tanks, which probably favored the transport of the agent and allowed its transmission to the spot-legged turtle. We thus confirmed presence of anti-Leptospira antibodies in chelonians maintained in the Rodrigues Alves Botanical Garden. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35377 10.5433/1679-0359.2019v40n6Supl3p3763 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35377 |
identifier_str_mv |
10.5433/1679-0359.2019v40n6Supl3p3763 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35377/26256 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 6Supl3 (2019); 3763-3768 Semina: Ciências Agrárias; v. 40 n. 6Supl3 (2019); 3763-3768 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1750315429478268928 |