Humoral immune response of horses experimentally infected with Trypanosoma evansi

Detalhes bibliográficos
Autor(a) principal: Marques, Luiz Carlos
Data de Publicação: 2001
Outros Autores: Machado, Rosângela Zacarias, Alessi, Antônio Carlos, Aquino, Lúcia Padilha Cury Thomaz de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2034
Resumo: Six adult horses were experimentally infected with Trypanosoma evansi (106 parasites). Three other adult horses served as negative control. Serum samples of the experimentally infected horses with T. evansi and non-infected controls horses were obtained before inoculation, and daily thereafter until 14 days post infection (DPI). After that time the serum samples were obtained weekly. Sera of the infected and non-infected control horses was tested by indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against T. evansi. Both ELISA and IFAT detected trypanosomal antibodies shortly after infection and showed progressive increases in antibodies levels during early stages of infection. The responses started on the eighth and eleventh DPI. Maximum IFAT and ELISA values were reached after four weeks of infection and were maintained at this level until the end of the period of study.
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spelling Humoral immune response of horses experimentally infected with Trypanosoma evansiResposta imune humoral de eqüinos infectados experimentalmente com Trypanosoma evansiTrypanosoma evansiTrypanosomiasisHorsesImmune response.Trypanosoma evansiTripanossomiaseEqüinosResposta imune.Six adult horses were experimentally infected with Trypanosoma evansi (106 parasites). Three other adult horses served as negative control. Serum samples of the experimentally infected horses with T. evansi and non-infected controls horses were obtained before inoculation, and daily thereafter until 14 days post infection (DPI). After that time the serum samples were obtained weekly. Sera of the infected and non-infected control horses was tested by indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against T. evansi. Both ELISA and IFAT detected trypanosomal antibodies shortly after infection and showed progressive increases in antibodies levels during early stages of infection. The responses started on the eighth and eleventh DPI. Maximum IFAT and ELISA values were reached after four weeks of infection and were maintained at this level until the end of the period of study.Seis eqüinos foram inoculados com 106 tripomastigota sangüícolas de Trypanosoma evansi. Três outros animais foram mantidos como testemunhas. Amostras de soro sangüíneo foram obtidas de todos os animais, antes da inoculação, e diariamente até o 14º dia pós inoculação (DPI); após este período uma vez por semana. Pesquisa de anticorpos anti- T. evansi, foram realizadas através da reação de imunofluorescência indireta (RIFI) e do ensaio de imunoabsorção enzimática (ELISA). A resposta imune humoral, detectada através da RIFI e do ELISA, iniciou-se, em média, a partir do oitavo DPI, alcançando títulos máximos após quatro semanas de evolução, e os titulos de anticorpos anti- T. evansi mantiveram-se elevadas até o término das observações. UEL2001-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/203410.5433/1679-0359.2001v22n2p131Semina: Ciências Agrárias; Vol. 22 No. 2 (2001); 131-133Semina: Ciências Agrárias; v. 22 n. 2 (2001); 131-1331679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2034/1775Marques, Luiz CarlosMachado, Rosângela ZacariasAlessi, Antônio CarlosAquino, Lúcia Padilha Cury Thomaz deinfo:eu-repo/semantics/openAccess2009-05-18T13:38:37Zoai:ojs.pkp.sfu.ca:article/2034Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2009-05-18T13:38:37Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Humoral immune response of horses experimentally infected with Trypanosoma evansi
Resposta imune humoral de eqüinos infectados experimentalmente com Trypanosoma evansi
title Humoral immune response of horses experimentally infected with Trypanosoma evansi
spellingShingle Humoral immune response of horses experimentally infected with Trypanosoma evansi
Marques, Luiz Carlos
Trypanosoma evansi
Trypanosomiasis
Horses
Immune response.
Trypanosoma evansi
Tripanossomiase
Eqüinos
Resposta imune.
title_short Humoral immune response of horses experimentally infected with Trypanosoma evansi
title_full Humoral immune response of horses experimentally infected with Trypanosoma evansi
title_fullStr Humoral immune response of horses experimentally infected with Trypanosoma evansi
title_full_unstemmed Humoral immune response of horses experimentally infected with Trypanosoma evansi
title_sort Humoral immune response of horses experimentally infected with Trypanosoma evansi
author Marques, Luiz Carlos
author_facet Marques, Luiz Carlos
Machado, Rosângela Zacarias
Alessi, Antônio Carlos
Aquino, Lúcia Padilha Cury Thomaz de
author_role author
author2 Machado, Rosângela Zacarias
Alessi, Antônio Carlos
Aquino, Lúcia Padilha Cury Thomaz de
author2_role author
author
author
dc.contributor.author.fl_str_mv Marques, Luiz Carlos
Machado, Rosângela Zacarias
Alessi, Antônio Carlos
Aquino, Lúcia Padilha Cury Thomaz de
dc.subject.por.fl_str_mv Trypanosoma evansi
Trypanosomiasis
Horses
Immune response.
Trypanosoma evansi
Tripanossomiase
Eqüinos
Resposta imune.
topic Trypanosoma evansi
Trypanosomiasis
Horses
Immune response.
Trypanosoma evansi
Tripanossomiase
Eqüinos
Resposta imune.
description Six adult horses were experimentally infected with Trypanosoma evansi (106 parasites). Three other adult horses served as negative control. Serum samples of the experimentally infected horses with T. evansi and non-infected controls horses were obtained before inoculation, and daily thereafter until 14 days post infection (DPI). After that time the serum samples were obtained weekly. Sera of the infected and non-infected control horses was tested by indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against T. evansi. Both ELISA and IFAT detected trypanosomal antibodies shortly after infection and showed progressive increases in antibodies levels during early stages of infection. The responses started on the eighth and eleventh DPI. Maximum IFAT and ELISA values were reached after four weeks of infection and were maintained at this level until the end of the period of study.
publishDate 2001
dc.date.none.fl_str_mv 2001-02-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2034
10.5433/1679-0359.2001v22n2p131
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2034
identifier_str_mv 10.5433/1679-0359.2001v22n2p131
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2034/1775
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 22 No. 2 (2001); 131-133
Semina: Ciências Agrárias; v. 22 n. 2 (2001); 131-133
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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