Avaliação da qualidade dos gametas de tambaqui (Colossoma macropomum) ao longo da estação reprodutiva

• Autor(a) principal: Galo, Juliana Minardi
• Data de Publicação: 2013
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
• Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/1496
• topic: Tambaqui; (Colossoma macropomum); Characidae; Parâmetros seminais; Período reprodutivo; Plasma seminal; Oócitos; Brasil.; Amazonian fish; (Colossoma macropomum); Characidae; Seminal parameters; Reproductive period; Seminal plasma; Oocytes; Brazil.; Ciências Agrárias; Zootecnia

The objective, in this work, was to analyze the reproductive behavior of the Colossoma macropomum specie, on quality of its male and female gametes throughout along the reproductive station, in order to determine the best time to accomplish the reproduction. Besides, it was analyzed the interaction among the presence of proteins of the seminal plasma of the C. macropomum as an indicators of seminal quality post-thawing. The experiment was carried out in Pimenta Bueno-Rondônia, in the reproductive station of November of 2009 to January of 2010, and November of 2010 to March of 2011. 23 males of C. macropomum were used during the reproductive station of 2009-2010, and 36 females in the station of 2010-2011. The male gametes were collected for the quali-quantitativas analyses along the reproductive station, every 15±5 days, the same happened with the collection of the feminine gametes, being classified as: period (1) - beginning of November (11/09); (2) - final of November (11/25); (3) - middle of December (12/14); (4) - beginning of January (01/03), and (5) - final of January (01/22); (6) - beginning of February (02/07); (7) - final of February (02/23); (8) - beginning of March (03/10) and (9) - final of March (03/28). During the evaluations of the spermatic quality in the periods three (middle of December) and four (beginning of January) from 2009/2010, an aliquot of semen of each animal was cry preserved, using Beltsville Thawing Solution (BTS) with 8% DMSO. A sample of 200 μL of semen of each animal, was diluted in 800 μL of BTS, and centrifuged in 800 rpm, and only the supernatant (proteins) was cry preserved to subsequent analysis of the protein outline of the seminal plasma, through the one-dimensional electrophoresis (SDS-PAGE). After 15 days the cryopreservation, a "pallet" with cry preserved semen was thawed for analysis of the quali-quantitative parameters. For the sperm parameters, it was not verified (P>0.05) period effect inside station for semen volume, spermatic vigor, time motility and spermatic concentration. When the progressive motility was evaluated among the collection periods, the effect (P <0.05) found was of quadratic behavior. About the sperm morphology significant differences were verified (P <0.05) in the probability of occurrences of normal sperms, primary and secondary abnormalities in function of the collection period. The fertilization rate and hatching presented significant effect (P <0.05) inside the period. For the qualitative parameters of the female gametes during the season 2010-2011, it was verified (P <0.05) period effect (it collects) inside the season for amount of oocytes, productive index and fertilization rate (P <0.05). In spite of the period 3 (collection - month of December) had not differentiated (P>0.05) from some periods, it was the period that presented the best established parameters for the oocytes'quality . For the analysis of the seminal plasma, considering all the collections, SDS-PAGE identified 15 protein bands (12, 25, 29, 34, 37, 40, 44, 50, 65, 70, 75, 78, 85, 90 and 100 kDa). When the interaction was evaluated (presence or absence) of the proteins found in the seminal plasma, with the sperm parameters post-thawing, great influence of the presence of the proteins was observed for sperm quality. Most of the proteins found in the seminal plasma influenced (P <0.05) the progressive motility of the frozen semen with DMSO. In consequence, a larger fertilization rate was observed (P <0.05) with the presence of the proteins 12, 34, 44, 85 and 90 kDa. In conclusion, the qualitative parameters of the semen and oócitos of C. macropomum presented changes during the reproductive station. The changes in the qualitative parameters of the semen can be associated to the aging processes of the sperms in the end of the reproductive station that can take a consequent reduction of the fertilization rates and hatching. The weight of liberated oocytes, production index and fertilization rate, had better results observed in the periods of December and January, indicating a better time to place the species reproduction. The proteins of the seminal plasma of C. macropomum influence the sperm quality post-thawing, as well as they can be used as indicators for the sperm quality post-thawing, mainly the proteins with molecular weight 50 kDa.