Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444

Detalhes bibliográficos
Autor(a) principal: Machado-Santos, Clarice
Data de Publicação: 2013
Outros Autores: Teixeira, Monalisa Jales, Sales, Armando, Abidu-Figueiredo, Marcelo
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18444
Resumo: The broad-snouted caiman, Caiman latirostris, is widely distributed in countries of South America. In Brazil it is considered an endangered species because of natural habitat destruction and illegal hunting.  In reptiles, the thyroid gland plays an integral part in ecdysis, reproduction, tail regeneration, growth, endocrine function and metabolism rate. The aim of the present study was to characterize the thyroid gland morphology of C. latirostris, based on histochemical and immunohistochemical techniques. The thyroid glands were fixed in Bouin's fluid and serial cross sections were stained with hematoxylin-eosin, Mallory’s trichrome, periodic acid-Schiff (PAS) and Alcian blue (AB pH 1.5 and 2.5). The immunohistochemical technique for 5-HT-IR cells was used. The thyroid gland has a dense irregular connective tissue forms a capsule enveloping the gland. There are several follicular acini of varying size lined by simple cuboidal or columnar epithelium in the thyroid gland. The follicles are connected by interfollicular connective tissue which contains blood vessels. We observed the presence of lymph nodes around the entire gland. There was a positive PAS reaction and negative AB reaction in the colloid. 5-HT-IR cells were detected around the follicle cells. No striking morphological differences were observed between C. latirostris and other domesticated mammals.  
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spelling Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444Thyroidhistologyreptiles5-HTHistologiaThe broad-snouted caiman, Caiman latirostris, is widely distributed in countries of South America. In Brazil it is considered an endangered species because of natural habitat destruction and illegal hunting.  In reptiles, the thyroid gland plays an integral part in ecdysis, reproduction, tail regeneration, growth, endocrine function and metabolism rate. The aim of the present study was to characterize the thyroid gland morphology of C. latirostris, based on histochemical and immunohistochemical techniques. The thyroid glands were fixed in Bouin's fluid and serial cross sections were stained with hematoxylin-eosin, Mallory’s trichrome, periodic acid-Schiff (PAS) and Alcian blue (AB pH 1.5 and 2.5). The immunohistochemical technique for 5-HT-IR cells was used. The thyroid gland has a dense irregular connective tissue forms a capsule enveloping the gland. There are several follicular acini of varying size lined by simple cuboidal or columnar epithelium in the thyroid gland. The follicles are connected by interfollicular connective tissue which contains blood vessels. We observed the presence of lymph nodes around the entire gland. There was a positive PAS reaction and negative AB reaction in the colloid. 5-HT-IR cells were detected around the follicle cells. No striking morphological differences were observed between C. latirostris and other domesticated mammals.  Universidade Estadual De Maringá2013-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1844410.4025/actascibiolsci.v35i4.18444Acta Scientiarum. Biological Sciences; Vol 35 No 4 (2013); 585-589Acta Scientiarum. Biological Sciences; v. 35 n. 4 (2013); 585-5891807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18444/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18444/pdf_1Machado-Santos, ClariceTeixeira, Monalisa JalesSales, ArmandoAbidu-Figueiredo, Marceloinfo:eu-repo/semantics/openAccess2022-11-23T17:34:11Zoai:periodicos.uem.br/ojs:article/18444Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:34:11Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444
title Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444
spellingShingle Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444
Machado-Santos, Clarice
Thyroid
histology
reptiles
5-HT
Histologia
title_short Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444
title_full Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444
title_fullStr Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444
title_full_unstemmed Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444
title_sort Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444
author Machado-Santos, Clarice
author_facet Machado-Santos, Clarice
Teixeira, Monalisa Jales
Sales, Armando
Abidu-Figueiredo, Marcelo
author_role author
author2 Teixeira, Monalisa Jales
Sales, Armando
Abidu-Figueiredo, Marcelo
author2_role author
author
author
dc.contributor.author.fl_str_mv Machado-Santos, Clarice
Teixeira, Monalisa Jales
Sales, Armando
Abidu-Figueiredo, Marcelo
dc.subject.por.fl_str_mv Thyroid
histology
reptiles
5-HT
Histologia
topic Thyroid
histology
reptiles
5-HT
Histologia
description The broad-snouted caiman, Caiman latirostris, is widely distributed in countries of South America. In Brazil it is considered an endangered species because of natural habitat destruction and illegal hunting.  In reptiles, the thyroid gland plays an integral part in ecdysis, reproduction, tail regeneration, growth, endocrine function and metabolism rate. The aim of the present study was to characterize the thyroid gland morphology of C. latirostris, based on histochemical and immunohistochemical techniques. The thyroid glands were fixed in Bouin's fluid and serial cross sections were stained with hematoxylin-eosin, Mallory’s trichrome, periodic acid-Schiff (PAS) and Alcian blue (AB pH 1.5 and 2.5). The immunohistochemical technique for 5-HT-IR cells was used. The thyroid gland has a dense irregular connective tissue forms a capsule enveloping the gland. There are several follicular acini of varying size lined by simple cuboidal or columnar epithelium in the thyroid gland. The follicles are connected by interfollicular connective tissue which contains blood vessels. We observed the presence of lymph nodes around the entire gland. There was a positive PAS reaction and negative AB reaction in the colloid. 5-HT-IR cells were detected around the follicle cells. No striking morphological differences were observed between C. latirostris and other domesticated mammals.  
publishDate 2013
dc.date.none.fl_str_mv 2013-07-04
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dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18444
10.4025/actascibiolsci.v35i4.18444
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18444
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dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18444/pdf
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dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 35 No 4 (2013); 585-589
Acta Scientiarum. Biological Sciences; v. 35 n. 4 (2013); 585-589
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
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reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
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