IgG anti-Ige autoantibodies in visceral leishmaniasis

p. 101-103

Access type:openAccess
Publication Date:2002
Main Author: Atta, Ajax Mercês
Other Authors: Atta, Maria Luiza Brito de Sousa, D'Oliveira Junior, Argemiro, Almeida, Roque Pacheco de, Araujo, Maria Ilma Andrade Santos, Carvalho Filho, Edgar Marcelino de
Document type: Article
Portuguese subjects:
Online Access:http://www.repositorio.ufba.br/ri/handle/ri/7598
Portuguese abstract:Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa e-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.