Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast

Detalhes bibliográficos
Autor(a) principal: Guimarães, Rafaela Araújo
Data de Publicação: 2017
Outros Autores: Lobo, Valácia Lemes da Silva, Côrtes, Márcio Vinícius Carvalho Barros, de Filippi, Marta Cristina Corsi, Prabhu, Anne Sitarama
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Tropical (Online)
Texto Completo: https://revistas.ufg.br/pat/article/view/42738
Resumo: The integrated management of rice leaf blast (Magnaporthe oryzae) is carried out mainly with the adoption of chemical control. However, the search for alternative practices has grown in recent years. Thus, the variability of 28 Sarocladium oryzae isolates was evaluated for the cerulenin production, as well as its potential for reducing the severity of rice leaf blast, quantifying the activity of enzymes linked to the plant defense mechanisms. More than 55 % of the S. oryzae isolates were antagonistic to the pathogens M. oryzae, Cochliobolus miyabeanus, Thanatephorus cucumeris and Monographella albescens, and 60 % of the isolates produced cerulenin at detectable levels. Both BRM 6461 (296.0 ?g mL-1) and BRM 6493 (undetectable cerulenin) inhibited the formation of M. oryzae appressoria in 89.5 % and 85 %, respectively. The BRM 6461 isolate, applied as conidial suspension and filtered, reduced the severity of rice leaf blast in 68.8 % and 75.5 %, respectively. The enzymatic activity in the presence of M. oryzae was higher for lipoxygenase at 5 h (filtered) and at 24 h and 72 h (conidial suspension) after the pathogen inoculation. For phenylalanine ammonia lyase, the highest expression was at 5 h (filtered) and 72 h (conidial suspension). The enzymes chitinase, ?-1,3-glucanase and peroxidase and the salicylic acid phytohormone presented no differences, in relation to the controls (water and M. oryzae). The filtered from the BRM 6461 isolate, basically constituted by cerulenin, reduced the severity of rice leaf blast and possibly activated the defense mechanisms of the rice plants against M. oryzae.
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spelling Characterization of Sarocladium oryzae and its reduction potential of rice leaf blastCaracterização de Sarocladium oryzae e seu potencial na redução de brusone foliar em arrozMagnaporthe oryzaeceruleninbiocontrolenzyme activity.Magnaporthe oryzaeceruleninabiocontroleatividade enzimática.The integrated management of rice leaf blast (Magnaporthe oryzae) is carried out mainly with the adoption of chemical control. However, the search for alternative practices has grown in recent years. Thus, the variability of 28 Sarocladium oryzae isolates was evaluated for the cerulenin production, as well as its potential for reducing the severity of rice leaf blast, quantifying the activity of enzymes linked to the plant defense mechanisms. More than 55 % of the S. oryzae isolates were antagonistic to the pathogens M. oryzae, Cochliobolus miyabeanus, Thanatephorus cucumeris and Monographella albescens, and 60 % of the isolates produced cerulenin at detectable levels. Both BRM 6461 (296.0 ?g mL-1) and BRM 6493 (undetectable cerulenin) inhibited the formation of M. oryzae appressoria in 89.5 % and 85 %, respectively. The BRM 6461 isolate, applied as conidial suspension and filtered, reduced the severity of rice leaf blast in 68.8 % and 75.5 %, respectively. The enzymatic activity in the presence of M. oryzae was higher for lipoxygenase at 5 h (filtered) and at 24 h and 72 h (conidial suspension) after the pathogen inoculation. For phenylalanine ammonia lyase, the highest expression was at 5 h (filtered) and 72 h (conidial suspension). The enzymes chitinase, ?-1,3-glucanase and peroxidase and the salicylic acid phytohormone presented no differences, in relation to the controls (water and M. oryzae). The filtered from the BRM 6461 isolate, basically constituted by cerulenin, reduced the severity of rice leaf blast and possibly activated the defense mechanisms of the rice plants against M. oryzae.O manejo integrado de brusone (Magnaporthe oryzae) em arroz é realizado, principalmente, com a adoção de controle químico. No entanto, a busca por práticas alternativas tem crescido nos últimos anos. Assim, avaliou-se a variabilidade de 28 isolados de Sarocladium oryzae, quanto à produção de cerulenina, e o seu potencial na redução da severidade de brusone foliar em arroz, quantificando-se a atividade de enzimas ligadas ao mecanismo de defesa das plantas. Mais de 55 % dos isolados de S. oryzae foram antagônicos aos patógenos M. oryzae, Cochliobolus miyabeanus, Thanatephorus cucumeris e Monographella albescens, e 60 % dos isolados produziram cerulenina em níveis detectáveis. Tanto BRM 6461 (296,0 µg mL-1) quanto BRM 6493 (cerulenina não detectável) inibiram a formação de apressórios de M. oryzae em 89,5 % e 85 %, respectivamente. O isolado BRM 6461, aplicado na forma de suspensão de conídios e filtrado, reduziu a severidade da brusone em 68,8 % e 75,5 %, respectivamente. A atividade enzimática, na presença de M. oryzae, foi maior para lipoxigenase após 5 h (filtrado) e 24 h e 72 h (suspensão de conídios) da inoculação do patógeno. Para fenilalanina-amônia liase, a maior expressão ocorreu após 5 h (filtrado) e 72 h (suspensão de conídios). As enzimas quitinase, ?-1,3-glucanase e peroxidase e o fitohormônio ácido salicílico não apresentaram diferenças em relação aos controles (água e M. oryzae). O filtrado do isolado BRM 6461, constituído basicamente por cerulenina, reduziu a severidade da brusone e, possivelmente, ativou os mecanismos de defesa da planta de arroz contra M. oryzae.Escola de Agronomia - Universidade Federal de Goiás2017-03-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/pat/article/view/42738Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 47, n. 1, Jan./Mar. 2017; 41-52Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 47, n. 1, jan./mar. 2017; 41-52Pesquisa Agropecuária Tropical; v. 47, n. 1, jan./mar. 2017; 41-521983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/42738/22598Copyright (c) 2017 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)info:eu-repo/semantics/openAccessGuimarães, Rafaela AraújoLobo, Valácia Lemes da SilvaCôrtes, Márcio Vinícius Carvalho Barrosde Filippi, Marta Cristina CorsiPrabhu, Anne Sitarama2020-07-13T18:26:52Zoai:ojs.revistas.ufg.br:article/42738Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:14.769761Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast
Caracterização de Sarocladium oryzae e seu potencial na redução de brusone foliar em arroz
title Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast
spellingShingle Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast
Guimarães, Rafaela Araújo
Magnaporthe oryzae
cerulenin
biocontrol
enzyme activity.
Magnaporthe oryzae
cerulenina
biocontrole
atividade enzimática.
title_short Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast
title_full Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast
title_fullStr Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast
title_full_unstemmed Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast
title_sort Characterization of Sarocladium oryzae and its reduction potential of rice leaf blast
author Guimarães, Rafaela Araújo
author_facet Guimarães, Rafaela Araújo
Lobo, Valácia Lemes da Silva
Côrtes, Márcio Vinícius Carvalho Barros
de Filippi, Marta Cristina Corsi
Prabhu, Anne Sitarama
author_role author
author2 Lobo, Valácia Lemes da Silva
Côrtes, Márcio Vinícius Carvalho Barros
de Filippi, Marta Cristina Corsi
Prabhu, Anne Sitarama
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, Rafaela Araújo
Lobo, Valácia Lemes da Silva
Côrtes, Márcio Vinícius Carvalho Barros
de Filippi, Marta Cristina Corsi
Prabhu, Anne Sitarama
dc.subject.por.fl_str_mv Magnaporthe oryzae
cerulenin
biocontrol
enzyme activity.
Magnaporthe oryzae
cerulenina
biocontrole
atividade enzimática.
topic Magnaporthe oryzae
cerulenin
biocontrol
enzyme activity.
Magnaporthe oryzae
cerulenina
biocontrole
atividade enzimática.
description The integrated management of rice leaf blast (Magnaporthe oryzae) is carried out mainly with the adoption of chemical control. However, the search for alternative practices has grown in recent years. Thus, the variability of 28 Sarocladium oryzae isolates was evaluated for the cerulenin production, as well as its potential for reducing the severity of rice leaf blast, quantifying the activity of enzymes linked to the plant defense mechanisms. More than 55 % of the S. oryzae isolates were antagonistic to the pathogens M. oryzae, Cochliobolus miyabeanus, Thanatephorus cucumeris and Monographella albescens, and 60 % of the isolates produced cerulenin at detectable levels. Both BRM 6461 (296.0 ?g mL-1) and BRM 6493 (undetectable cerulenin) inhibited the formation of M. oryzae appressoria in 89.5 % and 85 %, respectively. The BRM 6461 isolate, applied as conidial suspension and filtered, reduced the severity of rice leaf blast in 68.8 % and 75.5 %, respectively. The enzymatic activity in the presence of M. oryzae was higher for lipoxygenase at 5 h (filtered) and at 24 h and 72 h (conidial suspension) after the pathogen inoculation. For phenylalanine ammonia lyase, the highest expression was at 5 h (filtered) and 72 h (conidial suspension). The enzymes chitinase, ?-1,3-glucanase and peroxidase and the salicylic acid phytohormone presented no differences, in relation to the controls (water and M. oryzae). The filtered from the BRM 6461 isolate, basically constituted by cerulenin, reduced the severity of rice leaf blast and possibly activated the defense mechanisms of the rice plants against M. oryzae.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/pat/article/view/42738
url https://revistas.ufg.br/pat/article/view/42738
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.ufg.br/pat/article/view/42738/22598
dc.rights.driver.fl_str_mv Copyright (c) 2017 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics)
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
publisher.none.fl_str_mv Escola de Agronomia - Universidade Federal de Goiás
dc.source.none.fl_str_mv Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; v. 47, n. 1, Jan./Mar. 2017; 41-52
Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); v. 47, n. 1, jan./mar. 2017; 41-52
Pesquisa Agropecuária Tropical; v. 47, n. 1, jan./mar. 2017; 41-52
1983-4063
reponame:Pesquisa Agropecuária Tropical (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Pesquisa Agropecuária Tropical (Online)
collection Pesquisa Agropecuária Tropical (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv aseleguini.pat@gmail.com||mgoes@agro.ufg.br
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