CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL
Main Author: | |
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Publication Date: | 2015 |
Other Authors: | , , , , , |
Format: | Article |
Language: | por |
Source: | Ciência animal brasileira (Online) |
Download full: | https://revistas.ufg.br/vet/article/view/27457 |
Summary: | Cryotolerance of bovine oocytes and embryos maturated with addition of follicular fluid (LF) and ?-mercaptoethanol (BM) was evaluated. After vitrification, oocytes were maturated in: TCM-199 (control); BM (24h TCM-199+100µM BM); LF (6h in LF+18h TCM-199), and LF+BM (6h LF+18h TCM-199+100µM BM). There was not difference (p>0.05) in blastocysts rate in TCM (6.4%), BM (4.0%) and LF (3.4%) treatments. The hatching rates and cell density of hatched embryos did not differ (p>0.05) among treatments. In Experiment 2, hatched blastocysts (Bx) obtained in D7 or D8 were vitrified and evaluated according to its expanding and hatching rates. The expanding rate was similar (p>0.05), being observed a distinct pathway in hatching rate in D7 and D8 Bx. Higher hatching rate was observed in D7 Bx from control (TCM-54.2%) compared to BM (40.32%) and LF+BM (33.89%) treatments. The D8 Bx showed lower hatching rate in control (TCM-199) compared with D7 Bx. In BM, LF and LF+BM treatments, the hatching rate was similar for D7 or D8 embryos. Maturation with addition of LF and/or BM does not increase the oocyte or IVP embryo cryotolerance. Expanded blastocysts (D7) have higher cryotolerance and show a distinct pathway when added with LF or BM, in comparison with D8 embryos. Keywords: additives; cryotolerance; IVP embryos; maturation; vitrification. |
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CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOLCRIOTOLERÂNCIA DE OÓCITOS E EMBRIÕES BOVINOS MATURADOS COM LÍQUIDO FOLICULAR E/OU ?-MERCAPTOETANOLAnimal Reproductionvitrificaçãocriotolerânciaaditivosmaturaçãoembrião PIVCryotolerance of bovine oocytes and embryos maturated with addition of follicular fluid (LF) and ?-mercaptoethanol (BM) was evaluated. After vitrification, oocytes were maturated in: TCM-199 (control); BM (24h TCM-199+100µM BM); LF (6h in LF+18h TCM-199), and LF+BM (6h LF+18h TCM-199+100µM BM). There was not difference (p>0.05) in blastocysts rate in TCM (6.4%), BM (4.0%) and LF (3.4%) treatments. The hatching rates and cell density of hatched embryos did not differ (p>0.05) among treatments. In Experiment 2, hatched blastocysts (Bx) obtained in D7 or D8 were vitrified and evaluated according to its expanding and hatching rates. The expanding rate was similar (p>0.05), being observed a distinct pathway in hatching rate in D7 and D8 Bx. Higher hatching rate was observed in D7 Bx from control (TCM-54.2%) compared to BM (40.32%) and LF+BM (33.89%) treatments. The D8 Bx showed lower hatching rate in control (TCM-199) compared with D7 Bx. In BM, LF and LF+BM treatments, the hatching rate was similar for D7 or D8 embryos. Maturation with addition of LF and/or BM does not increase the oocyte or IVP embryo cryotolerance. Expanded blastocysts (D7) have higher cryotolerance and show a distinct pathway when added with LF or BM, in comparison with D8 embryos. Keywords: additives; cryotolerance; IVP embryos; maturation; vitrification.Foi avaliada a criotolerância de oócitos e embriões bovinos maturados com adição de líquido folicular (LF) e/ou ?-mercaptoetanol (BM). Após vitrificação, os oócitos foram maturados em: TCM-199 (controle); BM (24h TCM-199+100µM BM); LF (6h em LF+18h TCM-199) e LF+BM (6h LF+18h TCM-199+100µM BM). Não houve diferença (p>0,05) nas taxas de blastocistos dos tratamentos TCM (6,4%), BM (4,0%) e LF (3,4%). A eclosão e densidade celular dos embriões eclodidos não diferiram (p>0,05) nos tratamentos. No Experimento 2 blastocistos expandidos (Bx) obtidos em D7 ou D8 foram vitrificados, avaliando-se sua reexpansão e eclosão. A reexpansão foi semelhante (p>0,05), sendo observado comportamento distinto na eclosão entre Bx D7 e D8. Nos Bx D7 houve maior eclosão no controle (TCM–54,2%) em relação ao BM (40,32%) e LF+BM (33,89%). Os Bx D8 apresentaram menor eclosão no controle (TCM) em relação aos Bx D7. Nos tratamentos BM, LF e LF+BM a eclosão foi semelhante para Bx D7 ou D8. A maturação com adição de LF e/ou BM não melhora a criotolerância de oócitos imaturos e embriões PIV. Blastocistos expandidos precoces (D7) são mais criotolerantes e apresentam um comportamento distinto à adição de LF e BM, em relação aos tardios (D8). Palavras-chave: aditivos; criotolerância; embrião PIV; maturação; vitrificação.Universidade Federal de Goiás2015-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionrAvaliado por paresPesquisa Científicaapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/2745710.1590/cab.v16i2.27457Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 205-216Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 205-2161809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/27457/18506https://revistas.ufg.br/vet/article/view/27457/18507Gonçalves, Monalyza CadoriCesaro, Matheus PedrottiRodrigues, Murilo FariasLopes, Rui FélixMezzalira, Joana ClaudiaMozzaquatro, Fabricio DesconsiMezzalira, Alceuinfo:eu-repo/semantics/openAccess2015-05-22T12:39:36Zoai:ojs.revistas.ufg.br:article/27457Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:04.249409Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL CRIOTOLERÂNCIA DE OÓCITOS E EMBRIÕES BOVINOS MATURADOS COM LÍQUIDO FOLICULAR E/OU ?-MERCAPTOETANOL |
title |
CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL |
spellingShingle |
CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL Gonçalves, Monalyza Cadori Animal Reproduction vitrificação criotolerância aditivos maturação embrião PIV |
title_short |
CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL |
title_full |
CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL |
title_fullStr |
CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL |
title_full_unstemmed |
CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL |
title_sort |
CRYOTOLERANCE OF BOVINE OOCYTES AND EMBRYOS MATURATED WITH FOLLICULAR FLUID AND/OR ?-MERCAPTOETHANOL |
author |
Gonçalves, Monalyza Cadori |
author_facet |
Gonçalves, Monalyza Cadori Cesaro, Matheus Pedrotti Rodrigues, Murilo Farias Lopes, Rui Félix Mezzalira, Joana Claudia Mozzaquatro, Fabricio Desconsi Mezzalira, Alceu |
author_role |
author |
author2 |
Cesaro, Matheus Pedrotti Rodrigues, Murilo Farias Lopes, Rui Félix Mezzalira, Joana Claudia Mozzaquatro, Fabricio Desconsi Mezzalira, Alceu |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gonçalves, Monalyza Cadori Cesaro, Matheus Pedrotti Rodrigues, Murilo Farias Lopes, Rui Félix Mezzalira, Joana Claudia Mozzaquatro, Fabricio Desconsi Mezzalira, Alceu |
dc.subject.por.fl_str_mv |
Animal Reproduction vitrificação criotolerância aditivos maturação embrião PIV |
topic |
Animal Reproduction vitrificação criotolerância aditivos maturação embrião PIV |
description |
Cryotolerance of bovine oocytes and embryos maturated with addition of follicular fluid (LF) and ?-mercaptoethanol (BM) was evaluated. After vitrification, oocytes were maturated in: TCM-199 (control); BM (24h TCM-199+100µM BM); LF (6h in LF+18h TCM-199), and LF+BM (6h LF+18h TCM-199+100µM BM). There was not difference (p>0.05) in blastocysts rate in TCM (6.4%), BM (4.0%) and LF (3.4%) treatments. The hatching rates and cell density of hatched embryos did not differ (p>0.05) among treatments. In Experiment 2, hatched blastocysts (Bx) obtained in D7 or D8 were vitrified and evaluated according to its expanding and hatching rates. The expanding rate was similar (p>0.05), being observed a distinct pathway in hatching rate in D7 and D8 Bx. Higher hatching rate was observed in D7 Bx from control (TCM-54.2%) compared to BM (40.32%) and LF+BM (33.89%) treatments. The D8 Bx showed lower hatching rate in control (TCM-199) compared with D7 Bx. In BM, LF and LF+BM treatments, the hatching rate was similar for D7 or D8 embryos. Maturation with addition of LF and/or BM does not increase the oocyte or IVP embryo cryotolerance. Expanded blastocysts (D7) have higher cryotolerance and show a distinct pathway when added with LF or BM, in comparison with D8 embryos. Keywords: additives; cryotolerance; IVP embryos; maturation; vitrification. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion r Avaliado por pares Pesquisa Científica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/27457 10.1590/cab.v16i2.27457 |
url |
https://revistas.ufg.br/vet/article/view/27457 |
identifier_str_mv |
10.1590/cab.v16i2.27457 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/27457/18506 https://revistas.ufg.br/vet/article/view/27457/18507 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 16 No. 2 (2015); 205-216 Ciência Animal Brasileira / Brazilian Animal Science; v. 16 n. 2 (2015); 205-216 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788313792512 |