Extrato padronizado em compostos fenólicos de resíduos agroindustriais de goiaba em rações para frango

• Autor(a) principal: Noleto, Raiana Almeida
• Data de Publicação: 2018
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/8165
• topic: Atividade antioxidante; Avicultura; Psidium guajava L; Antioxidant activity; Poultry farming; Psidium guajava L; MEDICINA VETERINARIA::REPRODUCAO ANIMAL

Three experiments were carried out to evaluate guava extract standardized on phenolic compounds (GESPC) in broiler ration. In experiment one, were evaluated, total ash and insoluble in acids content, granulometric distribution and the intumescence rate of the plant, in liquid extract were evaluated total phenol content, flavonoids and tannins, antioxidant activity, centesimal composition and fatty acid profile was also evaluated. In experiment two, was evaluated performance, diet nutrients metabolizability, intestine histomorphometry and length, relative weight of digestible and lymphoid organs. A total of 300 male chickens were used, in a completely randomized design (CRD) with five treatments and six replicates with ten birds. Treatments: basal ration (control), basal ration + 120 mg/kg vitamin E or three levels of GESPC (600, 800 and 1000 mg/kg of feed). In experiment tree, performance, carcass yield and cuts, blood biochemistry, feed fatty acid profile, pH and meat color, were evaluated 24 hours after slaughter, lipid oxidation and fatty acid profile of breast and drumstick/thigh meat at 42 days old, after 30 and 60 days of storage. A total of 600 male chickens were used in a completely randomized design with five treatments, and five replicates with 24 birds. Treatment: Basal ration (control), basal ration with 200 mg/kg vitamin E or three levels of GESPC (1,000, 1,300 and 1,600 mg/kg of feed). In experiment one, plant sample presented 5.76% total ashes, 3.22% acid insoluble ashes, moderately thick powder and 2.6 mL of intumescence rate. The extract had 0.166 mg/mL total phenols, 5.97% flavonoids and 0.315 mg/mL of tannins, 96% of moisture, 2.6% proteins, 3.0% lipids and 12 fatty acids. The antioxidant activity: IC50 = 21.7 μg/mL by DPPH method and a percentage of 1.6 x 107 μM Fe 2 SO 4/g by FRAP method. In experiment two, chickens supplemented with extract had higher weight, final weight and better feed conversion at 7 days old. There was a quadratic effect for weight gain, final weight and a decrecent linear for feed conversion at seven days old. Diet nutrients metabolizability was not influenced. In broilers supplemented with 1000 mg/kg or 600 mg/kg of GESPC, intestine length and spleen weight was longer than control group at seven days old, respectively. Chickens fed with rations whit 800 and 1,000 mg/kg of GESPC had higher villi stature in relation to villus cryo in the duodenum in relation to control at 21 days old. In experiment tree carcass performance and yield were not influenced by treatments. Broilers supplemented with 1600 mg/kg of GESPC had higher total protein levels in blood compared to control group or 1,000 mg/kg group. There was a higher volume of globulins in broilers fed with 1600 mg/kg of GESPC, in relation to other treatments. Concentration of HDL was higher in broilers blood fed with ration containing 1,600 mg/kg of GESPC in relation to control groups and 1000 mg/kg of GESPC. A higher pH on broilers breast muscle was observed, when fed with 1300 or 1600 mg/kg of GESPC compared to vitamin E group. In the drumstick/thigh, pH was higher in broilers fed with on 1600 mg/kg of GESPC or vitamin E in relation to control group. The lipid oxidation at 30 days of storage was higher in drumstick/thigh of broilers supplemented with 1,300 and 1,600 mg/kg of GESPC in relation to other treatments. The lipid oxidation at 60 days of storage was higher in drumstick/thigh of broilers supplemented with 1,600 mg/kg of GESPC in relation to other treatments. The liquid extract of guava residue contains bioactive substances, with potential antioxidant activity by the DPPH and FRAP method. The GESPC 800 or 1,000 mg/kg supplementation level may be used in broiler diets considering intestinal performance and development. The EGPCF at doses of 1,300 and 1,600 mg/kg increases the lipid oxidation of broiler meat stored for 30 and 60 days.