Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tde/934 |
Resumo: | The wild animal trafficking is the third largest illegal activity in the world, ranking only behind the trafficking of weapons and drugs. The birds are the animals most affected by the illegal trade. Besides damaging to biodiversity, trafficking can also pose risks to human health. A number of diseases can be transmitted and acquired by the birds, the most commonly detected in the bacterial etiology. Improper management, especially related to transportation and overcrowding, promotes increased susceptibility to infections of birds or even the activation of latent infection with subsequent spread of pathogens. For these reasons this study aimed to conduct research and determine the Enterobacteriaceae resistance of strains of Escherichia coli, as well as raising the frequency of Chlamydophila spp. of parrots seized in actions to combat trafficking of wild animals in Goiás, with proper authorization from the Brazilian Institute of Environment and Renewable Natural Resources (IBAMA / GO). For this, 300 parrots in a period of one year were enrolled in the Screening Center of Wild Animals (CETAS), in Goiânia, Goiás, and underwent clinical and laboratory examinations. For Research Enterobacteriaceae were collected excreta from the bottom of cages, lined with aluminum foil and packed in ice for transport to the laboratory where they were examined by conventional bacteriological method. For the presence of Chlamydophila psittaci 300 cloacal swabs and 300 tracheids were collected and analyzed by polymerase chain reaction (PCR). We have identified the following excreta bacteria of the Enterobacteriaceae: Escherichia coli, 172/300 (33.87%), Enterobacter spp. 153/300 (30.12%), Klebsiella spp. 89/300 (17, 73%), Citrobacter spp. 9 / 300 (11.71%), Proteus vulgaris 21/300 (4.23%), Providencia alcalifaciens 5 / 300 (0.98%), Serratia sp.5/300 (0.98%), Hafnia aivei 3 / 300 ( 0.59%) and Salmonella sp. 1 / 300 (0.19%). Escherichia coli isolates were tested using the sensitivity to antibiotics, which revealed: amoxicillin (10mg) (70.93%), ampicillin (10mg) (75.58%), ciprofloxacin (5μg) (69.76%), chloramphenicol (30μg) (33.14% ), doxycycline (30μg) (64.53%), enrofloxacin (5μg) (41.28%), tetracycline (30μg) (69.19%), sulfonamide (300μg) (71.51%) of antimicrobial resistance. Of samples, 11/300 (3.66%) were positive in PCR analysis for Chlamydophilla spp. The results suggest that parrots from illegal trade are potential backers of zoonotic agents, and also point to the possibility that wild birds would constitute support for transfer of resistant phenotypes of E. coli to the human microbiota and other animals |
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ANDRADE, Maria Auxiliadorahttp://lattes.cnpq.br/9441751521255467JAYME, Valéria de Sáhttp://lattes.cnpq.br/0603234425928309LINHARES, Guido Fontgalland Coelhohttp://lattes.cnpq.br/6261928164195145http://lattes.cnpq.br/2231635369317203HIDASI, Hilari Wanderley2014-07-29T15:07:53Z2010-04-232010-02-26HIDASI, Hilari Wanderley. Detection of Enterobacteriaceae and Chlamydophila spp. in parrots from the distribution center of the Goiás Wildlife. 2010. 65 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2010.http://repositorio.bc.ufg.br/tede/handle/tde/934The wild animal trafficking is the third largest illegal activity in the world, ranking only behind the trafficking of weapons and drugs. The birds are the animals most affected by the illegal trade. Besides damaging to biodiversity, trafficking can also pose risks to human health. A number of diseases can be transmitted and acquired by the birds, the most commonly detected in the bacterial etiology. Improper management, especially related to transportation and overcrowding, promotes increased susceptibility to infections of birds or even the activation of latent infection with subsequent spread of pathogens. For these reasons this study aimed to conduct research and determine the Enterobacteriaceae resistance of strains of Escherichia coli, as well as raising the frequency of Chlamydophila spp. of parrots seized in actions to combat trafficking of wild animals in Goiás, with proper authorization from the Brazilian Institute of Environment and Renewable Natural Resources (IBAMA / GO). For this, 300 parrots in a period of one year were enrolled in the Screening Center of Wild Animals (CETAS), in Goiânia, Goiás, and underwent clinical and laboratory examinations. For Research Enterobacteriaceae were collected excreta from the bottom of cages, lined with aluminum foil and packed in ice for transport to the laboratory where they were examined by conventional bacteriological method. For the presence of Chlamydophila psittaci 300 cloacal swabs and 300 tracheids were collected and analyzed by polymerase chain reaction (PCR). We have identified the following excreta bacteria of the Enterobacteriaceae: Escherichia coli, 172/300 (33.87%), Enterobacter spp. 153/300 (30.12%), Klebsiella spp. 89/300 (17, 73%), Citrobacter spp. 9 / 300 (11.71%), Proteus vulgaris 21/300 (4.23%), Providencia alcalifaciens 5 / 300 (0.98%), Serratia sp.5/300 (0.98%), Hafnia aivei 3 / 300 ( 0.59%) and Salmonella sp. 1 / 300 (0.19%). Escherichia coli isolates were tested using the sensitivity to antibiotics, which revealed: amoxicillin (10mg) (70.93%), ampicillin (10mg) (75.58%), ciprofloxacin (5μg) (69.76%), chloramphenicol (30μg) (33.14% ), doxycycline (30μg) (64.53%), enrofloxacin (5μg) (41.28%), tetracycline (30μg) (69.19%), sulfonamide (300μg) (71.51%) of antimicrobial resistance. Of samples, 11/300 (3.66%) were positive in PCR analysis for Chlamydophilla spp. The results suggest that parrots from illegal trade are potential backers of zoonotic agents, and also point to the possibility that wild birds would constitute support for transfer of resistant phenotypes of E. coli to the human microbiota and other animalsO tráfico de animais silvestres é a terceira maior atividade ilegal do mundo, estando apenas atrás do tráfico de armas e de drogas. As aves são os animais mais atingidos pelo comércio ilegal. Além de prejudicial à biodiversidade, o tráfico também pode implicar riscos à saúde humana. Uma série de doenças podem ser transmitidas e adquiridas pelas aves, sendo as mais comumente detectadas as de etiologia bacteriana. O manejo inadequado, principalmente relacionado ao transporte e superpopulação, favorece o aumento da susceptibilidade das aves às infecções ou mesmo a ativação de infecções latentes com conseqüente disseminação de patógenos. Pelo exposto objetivouse realizar a pesquisa de Enterobacteriaceae e determinar o perfil de resistência das cepas isoladas de Escherichia coli, assim como, levantamento da freqüência de Chlamydophila spp. de psitacídeos apreendidos nas ações de combate ao tráfico de animais selvagens em Goiás, com devida autorização do Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA/GO). Para tanto, 300 psitacídeos, num período de um ano, foram cadastrados no Centro de Triagem de Animais Silvestres (CETAS), na cidade de Goiânia-Goiás, e submetidos a exames clínicos e laboratoriais. Para a pesquisa de Enterobacteriaceae foram colhidos excretas do fundo de gaiolas, forradas com papel alumínio e acondicionados em gelo para transporte ao laboratório, onde foram analisadas pelo método bacteriológico convencional. Para a pesquisa da Chlamydophila psittaci 300 suabes cloacais e 300 traqueais foram coletados e analisados pela reação em cadeia da polimerase (PCR). Identificaram-se nas excretas as seguintes bactérias da família Enterobacteriaceae: Escherichia coli, 172/300 (33,87%), Enterobacter spp. 153/300 (30,12%), Klebsiella spp. 89/300 (17, 73%), Citrobacter spp. 9/300 (11,71%), Proteus vulgaris 21/300 (4,23%), Providencia alcalifaciens 5/300 (0,98%), Serratia sp.5/300 (0,98%), Hafnia aivei 3/300 (0,59%) e Salmonella sp. 1/300 (0,19%). Isolados de Escherichia coli foram submetidos ao teste de sensibilidade à antimicrobianos, onde se obteve: amoxicilina (10μg) (70,93%), ampicilina (10μg) (75,58%), ciprofloxacina (5μg) (69,76%), cloranfenicol (30μg) (33,14%), doxiciclina (30μg) (64,53%), enrofloxacina(5μg) (41,28%), tetraciclina (30μg) (69,19%), sulfonamida (300μg) (71,51%) de resistência aos antimicrobianos testados. Das amostras colhidas, 11/300 (3,66%) foram positivas na análise pela PCR para Chlamydophilla spp. Os resultados sugerem que psitacídeos provenientes do comercio ilegal são potenciais veiculadores de agentes zoonóticos, e apontam ainda a possibilidade de aves selvagens se constituírem em suporte de transferência de fenótipos de E.coli resistentes para a microbiota humana e de outros animaisMade available in DSpace on 2014-07-29T15:07:53Z (GMT). No. of bitstreams: 1 Dissertacao Hilari _Pdf.pdf: 539492 bytes, checksum: e0488461a67ab589969fbb878d006e8c (MD5) Previous issue date: 2010-02-26application/pdfhttp://repositorio.bc.ufg.br/TEDE/retrieve/3724/Dissertacao%20Hilari%20_Pdf.pdf.jpgporUniversidade Federal de GoiásMestrado em Ciência AnimalUFGBRCiências Agrárias - VeterinariaE. coli, psitacose, resistência bacteriana, salmonelose, tráficoE. coli, psittacosis, bacterial resistance, salmonellosis, traffickingCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS)Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de GoiásDetection of Enterobacteriaceae and Chlamydophila spp. in parrots from the distribution center of the Goiás Wildlifeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertacao Hilari _Pdf.pdfapplication/pdf539492http://repositorio.bc.ufg.br/tede/bitstreams/39b6ee5b-e914-4fb6-a95b-fcc80e4357f2/downloade0488461a67ab589969fbb878d006e8cMD51THUMBNAILDissertacao Hilari _Pdf.pdf.jpgDissertacao Hilari _Pdf.pdf.jpgGenerated Thumbnailimage/jpeg1943http://repositorio.bc.ufg.br/tede/bitstreams/12baee41-6971-4ac7-8bd4-d269e06a55cb/downloadcc73c4c239a4c332d642ba1e7c7a9fb2MD52tde/9342014-07-30 03:07:36.277open.accessoai:repositorio.bc.ufg.br:tde/934http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2014-07-30T06:07:36Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)false |
dc.title.por.fl_str_mv |
Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás |
dc.title.alternative.eng.fl_str_mv |
Detection of Enterobacteriaceae and Chlamydophila spp. in parrots from the distribution center of the Goiás Wildlife |
title |
Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás |
spellingShingle |
Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás HIDASI, Hilari Wanderley E. coli, psitacose, resistência bacteriana, salmonelose, tráfico E. coli, psittacosis, bacterial resistance, salmonellosis, trafficking CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS) |
title_short |
Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás |
title_full |
Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás |
title_fullStr |
Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás |
title_full_unstemmed |
Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás |
title_sort |
Detecção de Enterobacteriaceae e Chlamydophila spp. em psitacídeos provenientes do centro de triagem de animais selvagens de Goiás |
author |
HIDASI, Hilari Wanderley |
author_facet |
HIDASI, Hilari Wanderley |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
ANDRADE, Maria Auxiliadora |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9441751521255467 |
dc.contributor.advisor-co1.fl_str_mv |
JAYME, Valéria de Sá |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0603234425928309 |
dc.contributor.advisor-co2.fl_str_mv |
LINHARES, Guido Fontgalland Coelho |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/6261928164195145 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2231635369317203 |
dc.contributor.author.fl_str_mv |
HIDASI, Hilari Wanderley |
contributor_str_mv |
ANDRADE, Maria Auxiliadora JAYME, Valéria de Sá LINHARES, Guido Fontgalland Coelho |
dc.subject.por.fl_str_mv |
E. coli, psitacose, resistência bacteriana, salmonelose, tráfico |
topic |
E. coli, psitacose, resistência bacteriana, salmonelose, tráfico E. coli, psittacosis, bacterial resistance, salmonellosis, trafficking CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS) |
dc.subject.eng.fl_str_mv |
E. coli, psittacosis, bacterial resistance, salmonellosis, trafficking |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA::SAUDE ANIMAL (PROGRAMAS SANITARIOS) |
description |
The wild animal trafficking is the third largest illegal activity in the world, ranking only behind the trafficking of weapons and drugs. The birds are the animals most affected by the illegal trade. Besides damaging to biodiversity, trafficking can also pose risks to human health. A number of diseases can be transmitted and acquired by the birds, the most commonly detected in the bacterial etiology. Improper management, especially related to transportation and overcrowding, promotes increased susceptibility to infections of birds or even the activation of latent infection with subsequent spread of pathogens. For these reasons this study aimed to conduct research and determine the Enterobacteriaceae resistance of strains of Escherichia coli, as well as raising the frequency of Chlamydophila spp. of parrots seized in actions to combat trafficking of wild animals in Goiás, with proper authorization from the Brazilian Institute of Environment and Renewable Natural Resources (IBAMA / GO). For this, 300 parrots in a period of one year were enrolled in the Screening Center of Wild Animals (CETAS), in Goiânia, Goiás, and underwent clinical and laboratory examinations. For Research Enterobacteriaceae were collected excreta from the bottom of cages, lined with aluminum foil and packed in ice for transport to the laboratory where they were examined by conventional bacteriological method. For the presence of Chlamydophila psittaci 300 cloacal swabs and 300 tracheids were collected and analyzed by polymerase chain reaction (PCR). We have identified the following excreta bacteria of the Enterobacteriaceae: Escherichia coli, 172/300 (33.87%), Enterobacter spp. 153/300 (30.12%), Klebsiella spp. 89/300 (17, 73%), Citrobacter spp. 9 / 300 (11.71%), Proteus vulgaris 21/300 (4.23%), Providencia alcalifaciens 5 / 300 (0.98%), Serratia sp.5/300 (0.98%), Hafnia aivei 3 / 300 ( 0.59%) and Salmonella sp. 1 / 300 (0.19%). Escherichia coli isolates were tested using the sensitivity to antibiotics, which revealed: amoxicillin (10mg) (70.93%), ampicillin (10mg) (75.58%), ciprofloxacin (5μg) (69.76%), chloramphenicol (30μg) (33.14% ), doxycycline (30μg) (64.53%), enrofloxacin (5μg) (41.28%), tetracycline (30μg) (69.19%), sulfonamide (300μg) (71.51%) of antimicrobial resistance. Of samples, 11/300 (3.66%) were positive in PCR analysis for Chlamydophilla spp. The results suggest that parrots from illegal trade are potential backers of zoonotic agents, and also point to the possibility that wild birds would constitute support for transfer of resistant phenotypes of E. coli to the human microbiota and other animals |
publishDate |
2010 |
dc.date.available.fl_str_mv |
2010-04-23 |
dc.date.issued.fl_str_mv |
2010-02-26 |
dc.date.accessioned.fl_str_mv |
2014-07-29T15:07:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
HIDASI, Hilari Wanderley. Detection of Enterobacteriaceae and Chlamydophila spp. in parrots from the distribution center of the Goiás Wildlife. 2010. 65 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tde/934 |
identifier_str_mv |
HIDASI, Hilari Wanderley. Detection of Enterobacteriaceae and Chlamydophila spp. in parrots from the distribution center of the Goiás Wildlife. 2010. 65 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2010. |
url |
http://repositorio.bc.ufg.br/tede/handle/tde/934 |
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Universidade Federal de Goiás |
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Mestrado em Ciência Animal |
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UFG |
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BR |
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Ciências Agrárias - Veterinaria |
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Universidade Federal de Goiás |
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