Detection of Campylobacter spp. in chilled and frozen broiler carcasses comparing immunoassay, PCR and real time PCR methods

• Autor(a) principal: Reis, Luciana Pimenta
• Data de Publicação: 2018
• Outros Autores: Menezes, Liliane Denize Miranda, Lima, Graciela Kunrath, Santos, Ethiene Luiza de Souza, Dorneles, Elaine Maria Seles, Assis, Débora Cristina Sampaio de, Lage, Andrey Pereira, Cançado, Silvana de Vasconcelos, Figueiredo, Tadeu Chaves de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFLA
• Texto Completo: http://repositorio.ufla.br/jspui/handle/1/33374
• topic: Reação em cadeia da polimerase; Campylobacter spp. - Métodos de detecção; C. jejuni - Contaminação; Polymerase Chain Reaction; Campylobacter spp. - Detection methods; C. jejuni - Contamination

In order to detect and identify Campylobacter spp. in broiler chicken carcasses, and to compare detection methods, 43 chilled and 43 frozen carcasses were collected and analyzed. Three methodologies were evaluated: an automated Enzyme Linked Fluorescent Assay (ELFA) VIDAS®30, Polymerase Chain Reaction (PCR) and real-time PCR. Only four chilled carcasses (4.6%) were considered positive for Campylobacter spp. by VIDAS®30 and no sample was positive when the conventional PCR technique was used. However, real-time PCR showed a higher incidence of contamination by Campylobacter spp. in broiler carcasses, with 45 (52.3%) positive samples. C. jejuni was the species most frequently reported in the samples (88.8%). No differences in the frequencies of Campylobacter spp. were observed between the chilled and frozen broiler carcasses. In conclusion, real-time PCR was the most sensitive method for the detection of Campylobacter spp. in chilled or frozen broiler carcasses, which were mainly contaminated by C. jejuni.