Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species

Detalhes bibliográficos
Autor(a) principal: Florentino, Ligiane Aparecida
Data de Publicação: 2010
Outros Autores: Sousa, Pedro Martins de, Silva, Jacqueline Savana, Silva, Karina Barroso, Moreira, Fatima Maria de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/28846
Resumo: The genetic diversity of ten Bradyrhizobium strains was evaluated for tolerance to high temperatures, to different salinity levels and for the efficiency of symbiosis with cowpea plants (Vigna unguiculata (L.) Walp.). Eight of these strains were isolated from nodules that appeared on cowpea after inoculation with suspensions of soil sampled from around the root system of Sesbania virgata (wand riverhemp) in ecosystems of South Minas Gerais. The other two strains used in our analyses as references, were from the Amazon and are currently recommended as cowpea inoculants. Genetic diversity was analyzed by amplifying repetitive DNA elements with the BOX primer, revealing high genetic diversity with each strain presenting a unique band profile. Leonard jar assays showed that the strains UFLA 03-30 and UFLA 03-38 had the highest N2-fixing potentials in symbiosis with cowpea. These strains had more shoot and nodule dry matter, more shoot N accumulation, and a higher relative efficiency than the strains recommended as inoculants. All strains grew in media of pH levels ranging from 4.0 to 9.0. The strains with the highest N2-fixing efficiencies in symbiosis with cowpea were also tolerant to the greatest number of antibiotics. However, these strains also had the lowest tolerance to high salt concentrations. All strains, with the exceptions of UFLA 03-84 and UFLA 03-37, tolerated temperatures of up to 40 ºC. The genetic and phenotypic characteristics of the eight strains isolated from soils of the same region were highly variable, as well as their symbiotic efficiencies, despite their common origin. This variability highlights the importance of including these tests in the selection of cowpea inoculant strains.
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spelling Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap speciesDiversidade e eficiência simbiótica de estirpes de bradyrhizobium capturadas próximo ao sistema radicular de sesbania virgata usando caupi como planta-iscaFixação biológica de nitrogênioEstirpes inoculantesDiversidade fenotípicaDiversidade genotípicaBiological nitrogen fixationInoculant strainsPhenotypic diversityGenotypic diversityThe genetic diversity of ten Bradyrhizobium strains was evaluated for tolerance to high temperatures, to different salinity levels and for the efficiency of symbiosis with cowpea plants (Vigna unguiculata (L.) Walp.). Eight of these strains were isolated from nodules that appeared on cowpea after inoculation with suspensions of soil sampled from around the root system of Sesbania virgata (wand riverhemp) in ecosystems of South Minas Gerais. The other two strains used in our analyses as references, were from the Amazon and are currently recommended as cowpea inoculants. Genetic diversity was analyzed by amplifying repetitive DNA elements with the BOX primer, revealing high genetic diversity with each strain presenting a unique band profile. Leonard jar assays showed that the strains UFLA 03-30 and UFLA 03-38 had the highest N2-fixing potentials in symbiosis with cowpea. These strains had more shoot and nodule dry matter, more shoot N accumulation, and a higher relative efficiency than the strains recommended as inoculants. All strains grew in media of pH levels ranging from 4.0 to 9.0. The strains with the highest N2-fixing efficiencies in symbiosis with cowpea were also tolerant to the greatest number of antibiotics. However, these strains also had the lowest tolerance to high salt concentrations. All strains, with the exceptions of UFLA 03-84 and UFLA 03-37, tolerated temperatures of up to 40 ºC. The genetic and phenotypic characteristics of the eight strains isolated from soils of the same region were highly variable, as well as their symbiotic efficiencies, despite their common origin. This variability highlights the importance of including these tests in the selection of cowpea inoculant strains.Dez estirpes de rizóbios, sendo oito isoladas de amostras de solos coletadas próximo ao sistema radicular de Sesbania virgata, no Sul de Minas Gerais, e duas recomendadas como inoculante para o caupi (Vigna unguiculata (L.) Walp.) usadas como referências, foram avaliadas quanto a: diversidade genética, eficiência simbiótica em caupi, tolerância a altas temperaturas e concentrações salinas, valores extremos de pH e 15 tipos de antibióticos. A análise de diversidade genética utilizando a técnica do Rep-PCR, com o primer BOX, revelou alta diversidade, pois cada estirpe apresentou perfil único de DNA. O teste de eficiência simbiótica conduzido em vasos de Leonard indicou que as estirpes UFLA 03-30 e UFLA 03-38 demonstraram alto potencial em fixar N2 em simbiose com o caupi, proporcionando resultados de matéria seca da parte aérea e dos nódulos, eficiência relativa e acúmulo de N na parte aérea superiores aos das estirpes recomendadas como inoculantes. Todas as estirpes cresceram em meios com valores de pH variando de 4,0 a 9,0. Em relação à tolerância aos antibióticos, foi observado que as estirpes eficientes em fixar N2 em simbiose com o caupi foram as que apresentaram tolerância a um maior número desses compostos. No entanto, estas estirpes mostraram comportamentos semelhantes em relação a tolerância a salinidade, constituindo o grupo de maior sensibilidade. Com exceção das estirpes UFLA 03-84 e UFLA 03-37, as demais toleraram até 40 ºC. Embora as estirpes estudadas tenham sido isoladas de solos da mesma região, com exceção das recomendadas para inoculante oriundas da Amazônia, foi observado que elas apresentaram comportamentos distintos quando submetidas aos diferentes testes de diversidade genética, fenotípica e simbiótica, justificando a importância de incluir estes testes no processo de seleção de estirpes simbióticas em caupi.Sociedade Brasileira de Ciência do Solo2018-03-16T13:32:59Z2018-03-16T13:32:59Z2010-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFLORENTINO, L. A. et al. Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 34, n. 4, p. 1113-1123, July/Aug. 2010.http://repositorio.ufla.br/jspui/handle/1/28846Revista Brasileira de Ciência do Soloreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessFlorentino, Ligiane AparecidaSousa, Pedro Martins deSilva, Jacqueline SavanaSilva, Karina BarrosoMoreira, Fatima Maria de Souzaeng2018-03-16T13:32:59Zoai:localhost:1/28846Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-03-16T13:32:59Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species
Diversidade e eficiência simbiótica de estirpes de bradyrhizobium capturadas próximo ao sistema radicular de sesbania virgata usando caupi como planta-isca
title Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species
spellingShingle Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species
Florentino, Ligiane Aparecida
Fixação biológica de nitrogênio
Estirpes inoculantes
Diversidade fenotípica
Diversidade genotípica
Biological nitrogen fixation
Inoculant strains
Phenotypic diversity
Genotypic diversity
title_short Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species
title_full Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species
title_fullStr Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species
title_full_unstemmed Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species
title_sort Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species
author Florentino, Ligiane Aparecida
author_facet Florentino, Ligiane Aparecida
Sousa, Pedro Martins de
Silva, Jacqueline Savana
Silva, Karina Barroso
Moreira, Fatima Maria de Souza
author_role author
author2 Sousa, Pedro Martins de
Silva, Jacqueline Savana
Silva, Karina Barroso
Moreira, Fatima Maria de Souza
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Florentino, Ligiane Aparecida
Sousa, Pedro Martins de
Silva, Jacqueline Savana
Silva, Karina Barroso
Moreira, Fatima Maria de Souza
dc.subject.por.fl_str_mv Fixação biológica de nitrogênio
Estirpes inoculantes
Diversidade fenotípica
Diversidade genotípica
Biological nitrogen fixation
Inoculant strains
Phenotypic diversity
Genotypic diversity
topic Fixação biológica de nitrogênio
Estirpes inoculantes
Diversidade fenotípica
Diversidade genotípica
Biological nitrogen fixation
Inoculant strains
Phenotypic diversity
Genotypic diversity
description The genetic diversity of ten Bradyrhizobium strains was evaluated for tolerance to high temperatures, to different salinity levels and for the efficiency of symbiosis with cowpea plants (Vigna unguiculata (L.) Walp.). Eight of these strains were isolated from nodules that appeared on cowpea after inoculation with suspensions of soil sampled from around the root system of Sesbania virgata (wand riverhemp) in ecosystems of South Minas Gerais. The other two strains used in our analyses as references, were from the Amazon and are currently recommended as cowpea inoculants. Genetic diversity was analyzed by amplifying repetitive DNA elements with the BOX primer, revealing high genetic diversity with each strain presenting a unique band profile. Leonard jar assays showed that the strains UFLA 03-30 and UFLA 03-38 had the highest N2-fixing potentials in symbiosis with cowpea. These strains had more shoot and nodule dry matter, more shoot N accumulation, and a higher relative efficiency than the strains recommended as inoculants. All strains grew in media of pH levels ranging from 4.0 to 9.0. The strains with the highest N2-fixing efficiencies in symbiosis with cowpea were also tolerant to the greatest number of antibiotics. However, these strains also had the lowest tolerance to high salt concentrations. All strains, with the exceptions of UFLA 03-84 and UFLA 03-37, tolerated temperatures of up to 40 ºC. The genetic and phenotypic characteristics of the eight strains isolated from soils of the same region were highly variable, as well as their symbiotic efficiencies, despite their common origin. This variability highlights the importance of including these tests in the selection of cowpea inoculant strains.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
2018-03-16T13:32:59Z
2018-03-16T13:32:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv FLORENTINO, L. A. et al. Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 34, n. 4, p. 1113-1123, July/Aug. 2010.
http://repositorio.ufla.br/jspui/handle/1/28846
identifier_str_mv FLORENTINO, L. A. et al. Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 34, n. 4, p. 1113-1123, July/Aug. 2010.
url http://repositorio.ufla.br/jspui/handle/1/28846
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência do Solo
publisher.none.fl_str_mv Sociedade Brasileira de Ciência do Solo
dc.source.none.fl_str_mv Revista Brasileira de Ciência do Solo
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
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institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
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