ATIVIDADE ANTIMICROBIANA DE NANOCÁPSULAS CONTENDO MONOLAURATO DE GLICEROL CONTRA ESPÉCIES DE Paenibacillus

• Autor(a) principal: Lopes, Leonardo Quintana Soares
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional Universidade Franciscana
• Texto Completo: http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/539
• topic: Loque americana; nanotecnologia; ecotoxicidade; American Foulbrood Disease, Nanotechnology, ecotoxicity; Biociências e Nanomateriais

The American Foulbrood Disease (AFB) is a fatal larval bee disease. The etiologic agent is the bacteria Paenibacillus larvae, being highly contagious due to the capacity of this microorganism to form spores. The treatment involves incineration of all contaminated materials, leading to high losses. The Glycerol Monolaurate (MLG) is a compound with a known antimicrobial potential, however its use is impaired due to the low solubility in water and the high melting point. In this context, the nanostructuration of some drugs offers advantages like better stability and solubility, increasing effectiveness and reducing toxic effects. The present study aimed to produce, characterize and evaluate the antimicrobial activity, the toxicity in bees and the ecotoxicity of MLG nanoparticles. The nanoparticles produced showed a diameter of 209.9 nm, polidispersion index of 0.044 and zeta potential of -23.4 mV. The study of the stability parameters showed that even after 120 days, the system was stable. The images of transmission electronic microscopy showed the nanometric size and spherical forms. The determination of inhibitory and bactericidal concentration showed that is necessary 142.8 μg/ml of the formulation (MLG nanoparticle) to obtain a bacteriostatic effect and of 285 μg/ml to obtain a bactericidal effect. The assay in bees showed that the free compound has a high toxicity while the nanoparticles showed a low toxic effect, reducing the number of dead bees. When analyzed the ecotoxicity, the springtail test soil results in high toxicity of the free compound, while the nanostructured formulation didn’t influence in mortality either in reproduction of organisms, showing that nanocapsules aren’t toxic to the ecosystem. Concluding, the developed formulation shows positive results in the action to combat AFB beyond not cause damage in bees and in soil, and can be future alternative to the treatment or prevention of AFB.