Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5

Detalhes bibliográficos
Autor(a) principal: Araujo, Itauá Leston
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFPel - Guaiaca
Texto Completo: http://guaiaca.ufpel.edu.br/handle/123456789/1235
Resumo: Glycoprotein D (gD) is essential for attachment and penetration of bovine herpesvirus type 5 (BoHV-5) into susceptible cells, and is a major target of host immune systems, inducing strong humoral and cellular immune responses. The immunogenicity of recombinant BoHV-5 (rgD5) expressed in Pichia pastoris was evaluated in bovines. Vaccines formulated with 100 μg rgD5 dose and adjuvants (Montanide ISA50V2 or Aluminum Hydroxide) with or without inactivated BoHV-5 or rgD5 without adjuvants were administered intramuscularly. A commercial vaccine was also used as control. The vaccine formulation rgD5 + ISA50V2 stimulated humoral immune responses after two doses, and higher titer of neutralizing antibodies were obtained in all three oil-based adjuvants formulations when compared to Hydroxide Aluminium adjuvant or the commercial vaccine. The vaccine BoHV-5 + rgD5 + ISA50V2 stimulated titers of neutralizing antibodies approximately 8 log2, which demonstrated higher correlations on the Indirect ELISA. Together, the results suggest that the recombinant gD5 conserved neutralizing epitopes and was able to stimulate a efficient humoral immune response in bovines.
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spelling 2014-08-20T13:32:50Z2014-05-292014-08-20T13:32:50Z2014-02-27ARAUJO, Itauá Leston. Induced humoral immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5. 2014. 59 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2014.http://guaiaca.ufpel.edu.br/handle/123456789/1235Glycoprotein D (gD) is essential for attachment and penetration of bovine herpesvirus type 5 (BoHV-5) into susceptible cells, and is a major target of host immune systems, inducing strong humoral and cellular immune responses. The immunogenicity of recombinant BoHV-5 (rgD5) expressed in Pichia pastoris was evaluated in bovines. Vaccines formulated with 100 μg rgD5 dose and adjuvants (Montanide ISA50V2 or Aluminum Hydroxide) with or without inactivated BoHV-5 or rgD5 without adjuvants were administered intramuscularly. A commercial vaccine was also used as control. The vaccine formulation rgD5 + ISA50V2 stimulated humoral immune responses after two doses, and higher titer of neutralizing antibodies were obtained in all three oil-based adjuvants formulations when compared to Hydroxide Aluminium adjuvant or the commercial vaccine. The vaccine BoHV-5 + rgD5 + ISA50V2 stimulated titers of neutralizing antibodies approximately 8 log2, which demonstrated higher correlations on the Indirect ELISA. Together, the results suggest that the recombinant gD5 conserved neutralizing epitopes and was able to stimulate a efficient humoral immune response in bovines.A glicoproteína D (gD) é essencial para ligação e penetração de herpesvírus bovino 5 (BoHV-5) em células suscetíveis, e é um alvo importante do sistema imune do hospedeiro, induzindo respostas imunes humoral e celular. A imunogenicidade da glicoproteína D recombinante de BoHV-5 (rgD5) expressa em Pichia pastoris foi avaliada em bovinos. Vacinas formuladas com 100 μg de rgD5 por dose e adjuvante a base de óleo (Montanide ISA50V2) ou hidróxido de alumínio, com ou sem adição de BoHV-5 inativado foram administradas via intramuscular, e uma vacina comercial utilizada como controle. A vacina rgD5 + ISA50V2 estimulou resposta imune humoral após duas doses, e os mais elevados títulos de anticorpos neutralizantes foram obtidos em todas as três formulações de adjuvantes à base de óleo quando comparado com a formulação de vacinas de adjuvante de hidróxido de alumínio e vacina comercial. A vacina BoHV-5 + rgD5 + ISA50V2 estimulou títulos de anticorpos neutralizantes aproximadamente a 8 log2, demonstrando correlação significativa com o ELISA indireto. Em conjunto, os resultados sugerem que a rgD5 conservou epítopos neutralizantes e foi capaz de estimular uma resposta imune humoral eficiente em bovinos.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em BiotecnologiaUFPelBRBiotecnologiaOil adjuvantPichia pastorisELISAAlphaherpesvirusesSubunit vaccineAdjuvante oleosoPichia pastorisELISAAlfaherpesvírusVacina de subunidadeCNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIAResposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5Induced humoral immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/2356280457157490http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763076J5Leite, Fabio Pereira LeivasAraujo, Itauá Lestoninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_itaua_leston_araujo.pdfapplication/pdf980896http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1235/1/dissertacao_itaua_leston_araujo.pdf4007b4d62827e236b56c4fe512f94f29MD51open accessTEXTdissertacao_itaua_leston_araujo.pdf.txtdissertacao_itaua_leston_araujo.pdf.txtExtracted Texttext/plain103158http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1235/2/dissertacao_itaua_leston_araujo.pdf.txt0839615d5f947743ba6c3c646a1d6254MD52open accessTHUMBNAILdissertacao_itaua_leston_araujo.pdf.jpgdissertacao_itaua_leston_araujo.pdf.jpgGenerated Thumbnailimage/jpeg1438http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1235/3/dissertacao_itaua_leston_araujo.pdf.jpga8c38648e44609ee758cdb232dc34726MD53open access123456789/12352019-08-23 10:42:49.779open accessoai:guaiaca.ufpel.edu.br:123456789/1235Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-23T13:42:49Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false
dc.title.por.fl_str_mv Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5
dc.title.alternative.eng.fl_str_mv Induced humoral immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5
title Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5
spellingShingle Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5
Araujo, Itauá Leston
Oil adjuvant
Pichia pastoris
ELISA
Alphaherpesviruses
Subunit vaccine
Adjuvante oleoso
Pichia pastoris
ELISA
Alfaherpesvírus
Vacina de subunidade
CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA
title_short Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5
title_full Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5
title_fullStr Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5
title_full_unstemmed Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5
title_sort Resposta imune humoral em bovinos induzida pela glicoproteína D recombinante de herpesvírus bovino tipo 5
author Araujo, Itauá Leston
author_facet Araujo, Itauá Leston
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/2356280457157490
dc.contributor.advisorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763076J5
dc.contributor.advisor1.fl_str_mv Leite, Fabio Pereira Leivas
dc.contributor.author.fl_str_mv Araujo, Itauá Leston
contributor_str_mv Leite, Fabio Pereira Leivas
dc.subject.eng.fl_str_mv Oil adjuvant
Pichia pastoris
ELISA
Alphaherpesviruses
Subunit vaccine
topic Oil adjuvant
Pichia pastoris
ELISA
Alphaherpesviruses
Subunit vaccine
Adjuvante oleoso
Pichia pastoris
ELISA
Alfaherpesvírus
Vacina de subunidade
CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA
dc.subject.por.fl_str_mv Adjuvante oleoso
Pichia pastoris
ELISA
Alfaherpesvírus
Vacina de subunidade
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA
description Glycoprotein D (gD) is essential for attachment and penetration of bovine herpesvirus type 5 (BoHV-5) into susceptible cells, and is a major target of host immune systems, inducing strong humoral and cellular immune responses. The immunogenicity of recombinant BoHV-5 (rgD5) expressed in Pichia pastoris was evaluated in bovines. Vaccines formulated with 100 μg rgD5 dose and adjuvants (Montanide ISA50V2 or Aluminum Hydroxide) with or without inactivated BoHV-5 or rgD5 without adjuvants were administered intramuscularly. A commercial vaccine was also used as control. The vaccine formulation rgD5 + ISA50V2 stimulated humoral immune responses after two doses, and higher titer of neutralizing antibodies were obtained in all three oil-based adjuvants formulations when compared to Hydroxide Aluminium adjuvant or the commercial vaccine. The vaccine BoHV-5 + rgD5 + ISA50V2 stimulated titers of neutralizing antibodies approximately 8 log2, which demonstrated higher correlations on the Indirect ELISA. Together, the results suggest that the recombinant gD5 conserved neutralizing epitopes and was able to stimulate a efficient humoral immune response in bovines.
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-08-20T13:32:50Z
dc.date.available.fl_str_mv 2014-05-29
2014-08-20T13:32:50Z
dc.date.issued.fl_str_mv 2014-02-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAUJO, Itauá Leston. Induced humoral immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5. 2014. 59 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2014.
dc.identifier.uri.fl_str_mv http://guaiaca.ufpel.edu.br/handle/123456789/1235
identifier_str_mv ARAUJO, Itauá Leston. Induced humoral immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5. 2014. 59 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2014.
url http://guaiaca.ufpel.edu.br/handle/123456789/1235
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade Federal de Pelotas
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dc.publisher.initials.fl_str_mv UFPel
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Biotecnologia
publisher.none.fl_str_mv Universidade Federal de Pelotas
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPel - Guaiaca
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