Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2052 |
Resumo: | Plant metabolism is sensitively to changes in oxygen availability by waterlogging. The immediate consequences of oxygen depletion are the activation of anaerobic metabolism and a reduction in energy production. This study aimed to evaluate the fermentative metabolism in soybean [Glycine max (L.) Merrill] roots and nodules under hypoxia and post-hypoxia conditions. Two experiments were conducted in greenhouse conditions with soybean genotypes Fundacep 53 RR and BRS Macota grown in 3L pots containing vermiculite as substrate, in association with Bradyrhizobium elkanii. The treatments were applied when the plants reached the R2 stage (reproductive). Experiment I - the root system of plants was subjected to hypoxia by fitting a second pot without holes and flooded with nutrient solution diluted to one-third of the original, in order to keep a blade of 20 mm on the surface of the substrate. Plants were maintained flooded for eight days, and so the pot without drilling holes was removed for recovery assessment. Non-waterlogging plants were maintained as control (normoxic condition). The activities of fermentation enzymes, alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC) and lactate dehydrogenase (LDH) were quantified in roots and nodules collected at 2, 4, 6 and 8 days after flooding or recovery . Experiment II - soybean plants were transferred to a hydroponic system and roots were subjected to hypoxia by flowing N2 gas during 24 and 72 h. For recovery, plants returned to vermiculite for 24 and 72 h. A control group of plants was maintained in vermiculite.The enzymatic activities of ADH, PDC and LDH, levels of anaerobic metabolites (ethanol, lactate and pyruvate) and carbohydrate content (sucrose, soluble sugars, starch and water soluble polysaccharides) were quantified in roots and nodules. The experiments were completely randomized with four replications and data were analyzed by ANOVA and compared by the Tukey test (P 0.05). Experiment I - activities of ADH, PDC and LDH increased significantly in roots and nodules of flooded plants and decreased during recovery. Fundacep 53 RR responded more effectively to treatments effects. Experiment II - the activity of ADH, PDC and LDH enzymes, as well as the content of ethanol and lactate, increased with hypoxia in roots and nodules, and returned to pre-hypoxic levels with the recovery in both genotypes. The pyruvate content increased in nodules and decreased in roots, sugars and sucrose levels increased in roots and decreased in nodules under hypoxia for both genotypes. Fundacep RR 53 was more responsive to the effects caused by hypoxia and post-hypoxia than BRS Macota. The effects were more pronounced in plants subjected to hypoxia by hydroponic system due to the rapid depletion of oxygen in the solution. |
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2014-08-20T13:59:15Z2012-01-252014-08-20T13:59:15Z2011-10-10BORELLA, Junior. Fermentative metabolism in soybean roots and nodules under hypoxia and post-hypoxia conditions. 2011. 62 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2011.http://repositorio.ufpel.edu.br/handle/ri/2052Plant metabolism is sensitively to changes in oxygen availability by waterlogging. The immediate consequences of oxygen depletion are the activation of anaerobic metabolism and a reduction in energy production. This study aimed to evaluate the fermentative metabolism in soybean [Glycine max (L.) Merrill] roots and nodules under hypoxia and post-hypoxia conditions. Two experiments were conducted in greenhouse conditions with soybean genotypes Fundacep 53 RR and BRS Macota grown in 3L pots containing vermiculite as substrate, in association with Bradyrhizobium elkanii. The treatments were applied when the plants reached the R2 stage (reproductive). Experiment I - the root system of plants was subjected to hypoxia by fitting a second pot without holes and flooded with nutrient solution diluted to one-third of the original, in order to keep a blade of 20 mm on the surface of the substrate. Plants were maintained flooded for eight days, and so the pot without drilling holes was removed for recovery assessment. Non-waterlogging plants were maintained as control (normoxic condition). The activities of fermentation enzymes, alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC) and lactate dehydrogenase (LDH) were quantified in roots and nodules collected at 2, 4, 6 and 8 days after flooding or recovery . Experiment II - soybean plants were transferred to a hydroponic system and roots were subjected to hypoxia by flowing N2 gas during 24 and 72 h. For recovery, plants returned to vermiculite for 24 and 72 h. A control group of plants was maintained in vermiculite.The enzymatic activities of ADH, PDC and LDH, levels of anaerobic metabolites (ethanol, lactate and pyruvate) and carbohydrate content (sucrose, soluble sugars, starch and water soluble polysaccharides) were quantified in roots and nodules. The experiments were completely randomized with four replications and data were analyzed by ANOVA and compared by the Tukey test (P 0.05). Experiment I - activities of ADH, PDC and LDH increased significantly in roots and nodules of flooded plants and decreased during recovery. Fundacep 53 RR responded more effectively to treatments effects. Experiment II - the activity of ADH, PDC and LDH enzymes, as well as the content of ethanol and lactate, increased with hypoxia in roots and nodules, and returned to pre-hypoxic levels with the recovery in both genotypes. The pyruvate content increased in nodules and decreased in roots, sugars and sucrose levels increased in roots and decreased in nodules under hypoxia for both genotypes. Fundacep RR 53 was more responsive to the effects caused by hypoxia and post-hypoxia than BRS Macota. The effects were more pronounced in plants subjected to hypoxia by hydroponic system due to the rapid depletion of oxygen in the solution.O metabolismo das plantas reage sensivelmente às mudanças de disponibilidade de oxigênio impostas pelo alagamento. Uma das consequências imediatas da depleção do oxigênio é a ativação do metabolismo anaeróbico e uma redução significativa na produção de energia. Este trabalho teve por objetivo avaliar o metabolismo fermentativo em raízes e nódulos de soja [Glycine max (L.) Merril] sob condições de hipoxia e pós-hipoxia. Para isso dois experimentos foram conduzidos em casa de vegetação com os genótipos Fundacep 53 RR e BRS Macota, cultivados em vasos de 3L contendo vermiculita como substrato, em associação com Bradyrhizobium elkanii. Os tratamentos foram aplicados quando as plantas atingiram o estádio R2 (reprodutivo). Experimento I - o sistema radicular da soja foi submetido à hipoxia pelo encaixe de um segundo vaso sem perfurações e inundado com solução nutritiva diluída a 1/3 da concentração original, de forma a se manter uma lâmina de 20 mm sobre a superfície do substrato. A inundação das plantas foi conduzida por 8 dias, após este período foi retirado os vasos sem perfurações para avaliação da recuperação. O material vegetal foi coletado no 2º, 4º, 6º e 8º dia após a inundação (DAI) ou recuperação (DAR), juntamente com seus respectivos controles (mantidos em normoxia) para a dosagem da atividade das enzimas fermentativas, álcool desidrogenase (ADH), piruvato descarboxilase (PDC) e lactato desidrogenase (LDH) em raízes e nódulos. Experimento II - as plantas foram transferidas para um sistema hidropônico e submetidas à hipoxia do sistema radicular pelo borbulhamento de N2 gasoso por 24 e 72 h ou mantidas em vermiculita (controle). Posteriormente retornaram para vermiculita sob condições de normoxia por 24 e 72 h. Foram avaliadas a atividade das enzimas ADH, PDC e LDH , metabólitos anaeróbicos (etanol, lactato e piruvato) e conteúdo de carboidratos (sacarose, açúcares solúveis totais, amido e polissacarídeos solúveis em água) em raízes e nódulos. O delineamento foi inteiramente casualizado com quatro repetições. Os dados foram submetidos à ANOVA e as médias comparadas pelo teste de Tukey a 5% de probabilidade. No experimento I a inundação promoveu aumento significativo da atividade da ADH, PDC e LDH, bem como a diminuição da atividade com a recuperação em raízes e nódulos. Fundacep 53 RR respondeu de forma mais efetiva aos tratamentos. No experimento II, a atividade das enzimas ADH, PDC e LDH, bem como os teores de etanol e lactato, aumentaram com a hipoxia em raízes e nódulos e retornaram aos níveis pré-hipóxicos com a recuperação em ambos os genótipos. O teor de piruvato aumentou nos nódulos e diminuiu nas raízes e os teores de açúcares e sacarose aumentaram nas raízes e diminuíram nos nódulos em hipoxia para ambos os genótipos. Fundacep 53 RR foi mais responsiva aos efeitos provocados pela hipoxia e pós-hipoxia que BRS Macota. Os efeitos foram mais pronunciados nas plantas submetidas à hipoxia pelo sistema hidropônico em função da rápida depleção do oxigênio na solução.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em Fisiologia VegetalUFPelBRBiologiaGlycine maxAlagamentoDéficit de oxigênioEnzimas fermentativasMetabólitosGlycine maxWaterloggingOxygen deficiencyFermentative enzymesMetabolitesCNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETALMetabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxiaFermentative metabolism in soybean roots and nodules under hypoxia and post-hypoxia conditionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/0414217126348513http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787491U0Amarante, Luciano dohttp://lattes.cnpq.br/2414061342279670Moraes, Dario Munt deBorella, Juniorinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_junior_borella.pdfapplication/pdf624661http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2052/1/dissertacao_junior_borella.pdfc2eaffe012f5616bb86c7276e9b33e73MD51open accessTEXTdissertacao_junior_borella.pdf.txtdissertacao_junior_borella.pdf.txtExtracted Texttext/plain101477http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2052/2/dissertacao_junior_borella.pdf.txteb8ea33802a7c9496bbdaa5bdb9d0365MD52open accessTHUMBNAILdissertacao_junior_borella.pdf.jpgdissertacao_junior_borella.pdf.jpgGenerated Thumbnailimage/jpeg1308http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2052/3/dissertacao_junior_borella.pdf.jpg9daa5dd10a3bd9d0c1b5dd2266782c38MD53open access123456789/20522019-09-09 11:56:16.483open accessoai:guaiaca.ufpel.edu.br:123456789/2052Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-09T14:56:16Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia |
dc.title.alternative.eng.fl_str_mv |
Fermentative metabolism in soybean roots and nodules under hypoxia and post-hypoxia conditions |
title |
Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia |
spellingShingle |
Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia Borella, Junior Glycine max Alagamento Déficit de oxigênio Enzimas fermentativas Metabólitos Glycine max Waterlogging Oxygen deficiency Fermentative enzymes Metabolites CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia |
title_full |
Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia |
title_fullStr |
Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia |
title_full_unstemmed |
Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia |
title_sort |
Metabolismo fermentativo em raízes e nódulos de soja sob condições de hipoxia e pós-hipoxia |
author |
Borella, Junior |
author_facet |
Borella, Junior |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0414217126348513 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787491U0 |
dc.contributor.advisor-co1.fl_str_mv |
Amarante, Luciano do |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2414061342279670 |
dc.contributor.advisor1.fl_str_mv |
Moraes, Dario Munt de |
dc.contributor.author.fl_str_mv |
Borella, Junior |
contributor_str_mv |
Amarante, Luciano do Moraes, Dario Munt de |
dc.subject.por.fl_str_mv |
Glycine max Alagamento Déficit de oxigênio Enzimas fermentativas Metabólitos |
topic |
Glycine max Alagamento Déficit de oxigênio Enzimas fermentativas Metabólitos Glycine max Waterlogging Oxygen deficiency Fermentative enzymes Metabolites CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.eng.fl_str_mv |
Glycine max Waterlogging Oxygen deficiency Fermentative enzymes Metabolites |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
description |
Plant metabolism is sensitively to changes in oxygen availability by waterlogging. The immediate consequences of oxygen depletion are the activation of anaerobic metabolism and a reduction in energy production. This study aimed to evaluate the fermentative metabolism in soybean [Glycine max (L.) Merrill] roots and nodules under hypoxia and post-hypoxia conditions. Two experiments were conducted in greenhouse conditions with soybean genotypes Fundacep 53 RR and BRS Macota grown in 3L pots containing vermiculite as substrate, in association with Bradyrhizobium elkanii. The treatments were applied when the plants reached the R2 stage (reproductive). Experiment I - the root system of plants was subjected to hypoxia by fitting a second pot without holes and flooded with nutrient solution diluted to one-third of the original, in order to keep a blade of 20 mm on the surface of the substrate. Plants were maintained flooded for eight days, and so the pot without drilling holes was removed for recovery assessment. Non-waterlogging plants were maintained as control (normoxic condition). The activities of fermentation enzymes, alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC) and lactate dehydrogenase (LDH) were quantified in roots and nodules collected at 2, 4, 6 and 8 days after flooding or recovery . Experiment II - soybean plants were transferred to a hydroponic system and roots were subjected to hypoxia by flowing N2 gas during 24 and 72 h. For recovery, plants returned to vermiculite for 24 and 72 h. A control group of plants was maintained in vermiculite.The enzymatic activities of ADH, PDC and LDH, levels of anaerobic metabolites (ethanol, lactate and pyruvate) and carbohydrate content (sucrose, soluble sugars, starch and water soluble polysaccharides) were quantified in roots and nodules. The experiments were completely randomized with four replications and data were analyzed by ANOVA and compared by the Tukey test (P 0.05). Experiment I - activities of ADH, PDC and LDH increased significantly in roots and nodules of flooded plants and decreased during recovery. Fundacep 53 RR responded more effectively to treatments effects. Experiment II - the activity of ADH, PDC and LDH enzymes, as well as the content of ethanol and lactate, increased with hypoxia in roots and nodules, and returned to pre-hypoxic levels with the recovery in both genotypes. The pyruvate content increased in nodules and decreased in roots, sugars and sucrose levels increased in roots and decreased in nodules under hypoxia for both genotypes. Fundacep RR 53 was more responsive to the effects caused by hypoxia and post-hypoxia than BRS Macota. The effects were more pronounced in plants subjected to hypoxia by hydroponic system due to the rapid depletion of oxygen in the solution. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-10-10 |
dc.date.available.fl_str_mv |
2012-01-25 2014-08-20T13:59:15Z |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:59:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BORELLA, Junior. Fermentative metabolism in soybean roots and nodules under hypoxia and post-hypoxia conditions. 2011. 62 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2052 |
identifier_str_mv |
BORELLA, Junior. Fermentative metabolism in soybean roots and nodules under hypoxia and post-hypoxia conditions. 2011. 62 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2011. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2052 |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Fisiologia Vegetal |
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UFPel |
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BR |
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Biologia |
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Universidade Federal de Pelotas |
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