Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://guaiaca.ufpel.edu.br/handle/123456789/1207 |
Resumo: | Rice (Oryza sativa L.) is one of the most important cereals in the world being cultivated in lowland ecosystems, which have a common characteristic: lack of drainage conditions. The lack of oxygen, due to submergence of seedlings, and iron toxicity are two of the major abiotic stresses which rice plants are subjected. Recently it was realized the importance of some ethylene responsive transcription factors (ERFs) in plant response to different stresses, especially to oxygen depletion. Considering it, two studies were made in order to evaluate the expression of ERFs under different stresses. In a first study seven genes were selected, based on previous results obtained from Genevestigator analysis, and had their transcriptional expression evaluated trough quantitative PCR (qPCR) in rice seedlings under oxygen depletion and iron overload. In the second study thirteen ERFs, which had no information available in the literature, also had their transcriptional levels evaluated in seedlings under conditions of hypoxia and anoxia through qPCR in a cultivar susceptible to flooding (Bonança) and a tolerant one (Nipponbare). As general conclusions it was once more emphasized the importance of ERF genes on plant response to environmental stresses. The specific function of each of these genes is yet to be studied. In silico analysis suggests the methylation of promoters as a possible way to regulate them. More studies about the function of these ERFs and about the role of methylation in plant stress response should be done. |
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2014-08-20T13:32:46Z2013-06-072014-08-20T13:32:46Z2012-07-27SANTOS, Railson Schreinert dos. Transcriptional and Epigenetic Regulation of ERFs in Rice Under Abiotic Stress. 2012. 100 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2012.http://guaiaca.ufpel.edu.br/handle/123456789/1207Rice (Oryza sativa L.) is one of the most important cereals in the world being cultivated in lowland ecosystems, which have a common characteristic: lack of drainage conditions. The lack of oxygen, due to submergence of seedlings, and iron toxicity are two of the major abiotic stresses which rice plants are subjected. Recently it was realized the importance of some ethylene responsive transcription factors (ERFs) in plant response to different stresses, especially to oxygen depletion. Considering it, two studies were made in order to evaluate the expression of ERFs under different stresses. In a first study seven genes were selected, based on previous results obtained from Genevestigator analysis, and had their transcriptional expression evaluated trough quantitative PCR (qPCR) in rice seedlings under oxygen depletion and iron overload. In the second study thirteen ERFs, which had no information available in the literature, also had their transcriptional levels evaluated in seedlings under conditions of hypoxia and anoxia through qPCR in a cultivar susceptible to flooding (Bonança) and a tolerant one (Nipponbare). As general conclusions it was once more emphasized the importance of ERF genes on plant response to environmental stresses. The specific function of each of these genes is yet to be studied. In silico analysis suggests the methylation of promoters as a possible way to regulate them. More studies about the function of these ERFs and about the role of methylation in plant stress response should be done.O arroz (Oryza sativa L.) é um dos cereais mais importantes no mundo, sendo muito cultivado em ecossistemas de várzea, os quais apresentam uma característica comum: condições de deficiência de drenagem. A falta de oxigênio, devido à submergência das plântulas, e a toxidez por excesso de ferro são dois dos maiores estresses abióticos dentre os quais as plantas de arroz são submetidas. Recentemente percebeu-se a importância de alguns fatores de transcrição responsivos ao etileno (ERFs) na resposta vegetal à diferentes estresses, em especial à deficiência de oxigênio. Considerando-se o exposto efetuaram-se basicamente dois estudos visando avaliar a expressão de ERFs em diferentes estresses. Em um primeiro estudo sete genes foram selecionados a partir de resultados anteriores obtidos de análise no Genevestigator e estes tiveram sua expressão transcricional avaliada por PCR quantitativa (qPCR) em plântulas de arroz sob deficiência de oxigênio e excesso de ferro. No segundo estudo treze ERFs, os quais não apresentavam informações disponíveis na literatura, também tiveram seus níveis de expressão transcricional avaliados em plântulas sob condições de estresse por hipoxia e anoxia, também através de qPCR em uma cv. sensível à inundação (Bonança) e uma tolerante (Nipponbare). Como conclusões gerais se ressalta, novamente, a importância dos ERFs na resposta vegetal frente a estresses ambientais. A função específica de cada um destes genes ainda necessita ser estudada. Uma análise in silico nos promotores destes genes indica a metilação como possível forma de regulação transcricional. Mais estudos sobre a função destes ERFs e sobre o papel da metilação na resposta de arroz a estresses deverão ser feitas.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em BiotecnologiaUFPelBRBiotecnologiaMethylationAbiotic stressPromotersMetilaçãoEstresse abióticoqPCRPromotoresCNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETALRegulação transcricional e epigenética de ERFs em arroz sob estresse abióticoTranscriptional and epigenetic regulation of ERFs in rice under abiotic stressinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/9720465154745120http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780819H4Oliveira, Antônio Costa deSantos, Railson Schreinert dosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_railson_schreinert_dos_santos.pdfapplication/pdf1331296http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1207/1/dissertacao_railson_schreinert_dos_santos.pdf2121f264ff3310f6f460595517aafdabMD51open accessTEXTdissertacao_railson_schreinert_dos_santos.pdf.txtdissertacao_railson_schreinert_dos_santos.pdf.txtExtracted Texttext/plain149265http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1207/2/dissertacao_railson_schreinert_dos_santos.pdf.txt0f73b249ce107e90756dba2ba1d19e3fMD52open accessTHUMBNAILdissertacao_railson_schreinert_dos_santos.pdf.jpgdissertacao_railson_schreinert_dos_santos.pdf.jpgGenerated Thumbnailimage/jpeg1820http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1207/3/dissertacao_railson_schreinert_dos_santos.pdf.jpg9a30177f3a11146d8a018b933fe72f75MD53open access123456789/12072019-08-23 10:41:41.632open accessoai:guaiaca.ufpel.edu.br:123456789/1207Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-23T13:41:41Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico |
dc.title.alternative.eng.fl_str_mv |
Transcriptional and epigenetic regulation of ERFs in rice under abiotic stress |
title |
Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico |
spellingShingle |
Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico Santos, Railson Schreinert dos Methylation Abiotic stress Promoters Metilação Estresse abiótico qPCR Promotores CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL |
title_short |
Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico |
title_full |
Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico |
title_fullStr |
Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico |
title_full_unstemmed |
Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico |
title_sort |
Regulação transcricional e epigenética de ERFs em arroz sob estresse abiótico |
author |
Santos, Railson Schreinert dos |
author_facet |
Santos, Railson Schreinert dos |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/9720465154745120 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780819H4 |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Antônio Costa de |
dc.contributor.author.fl_str_mv |
Santos, Railson Schreinert dos |
contributor_str_mv |
Oliveira, Antônio Costa de |
dc.subject.eng.fl_str_mv |
Methylation Abiotic stress Promoters |
topic |
Methylation Abiotic stress Promoters Metilação Estresse abiótico qPCR Promotores CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL |
dc.subject.por.fl_str_mv |
Metilação Estresse abiótico qPCR Promotores |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL |
description |
Rice (Oryza sativa L.) is one of the most important cereals in the world being cultivated in lowland ecosystems, which have a common characteristic: lack of drainage conditions. The lack of oxygen, due to submergence of seedlings, and iron toxicity are two of the major abiotic stresses which rice plants are subjected. Recently it was realized the importance of some ethylene responsive transcription factors (ERFs) in plant response to different stresses, especially to oxygen depletion. Considering it, two studies were made in order to evaluate the expression of ERFs under different stresses. In a first study seven genes were selected, based on previous results obtained from Genevestigator analysis, and had their transcriptional expression evaluated trough quantitative PCR (qPCR) in rice seedlings under oxygen depletion and iron overload. In the second study thirteen ERFs, which had no information available in the literature, also had their transcriptional levels evaluated in seedlings under conditions of hypoxia and anoxia through qPCR in a cultivar susceptible to flooding (Bonança) and a tolerant one (Nipponbare). As general conclusions it was once more emphasized the importance of ERF genes on plant response to environmental stresses. The specific function of each of these genes is yet to be studied. In silico analysis suggests the methylation of promoters as a possible way to regulate them. More studies about the function of these ERFs and about the role of methylation in plant stress response should be done. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-07-27 |
dc.date.available.fl_str_mv |
2013-06-07 2014-08-20T13:32:46Z |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:32:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Railson Schreinert dos. Transcriptional and Epigenetic Regulation of ERFs in Rice Under Abiotic Stress. 2012. 100 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
dc.identifier.uri.fl_str_mv |
http://guaiaca.ufpel.edu.br/handle/123456789/1207 |
identifier_str_mv |
SANTOS, Railson Schreinert dos. Transcriptional and Epigenetic Regulation of ERFs in Rice Under Abiotic Stress. 2012. 100 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
url |
http://guaiaca.ufpel.edu.br/handle/123456789/1207 |
dc.language.iso.fl_str_mv |
por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Biotecnologia |
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UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biotecnologia |
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Universidade Federal de Pelotas |
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