Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/2818 |
Resumo: | The use of the diversity of common bean (Phaseolus vulgaris L.) germplasm collections can provide materials with higher contribution of biological nitrogen fixation. The aim of this study was to identify, in germplasm bank of Embrapa Rice and Beans, common bean genotypes of Mesoamerican and Andean origin with potential for obtaining N2 by rhizobia inoculation. The evaluation consisted of two screenings of 879 genotypes in greenhouse. Further, 15 of these genotypes and two commercial cultivars were evaluated under field conditions. Greenhouse experiments were conducted in randomized block design with three replications in which 879 genotypes were evaluated in response to inoculation with commercial Rhizobium strains (SEMIA 4077, SEMIA 4080, SEMIA 4088). The cultivar Ouro Negro was used as reference. Pre-germinated seeds were seeded in 3 L pots with sterile sand and vermiculite. Plants were inoculated seven days after emergence (DAE), and sterile Norris solution without N was added weekly. The number of nodules, nodule dry weight and one nodule dry weight were determined 35 DAE. The percentage of genotypes that exceeded the reference was established, as 21% for number of nodules, 46% for nodule dry weight and 32% for one nodule dry weight. We selected 116 genotypes, which were evaluated in other experiment. A joint analysis of the data obtained in the first and second phases for these 116 genotypes was performed, and 15 genotypes were selected. These 15 genotypes and the commercial cultivars P?rola and Ouro Negro were evaluated at field conditions under two N sources, inoculation with rhizobia or fertilization with 80 kg N ha-1. Five experiments were carried out in Santo Ant?nio de Goi?s ? GO, Ponta Grossa ? PR and Serop?dica ? RJ. A split plot design in randomized complete block was used with N sources as main plots and genotypes as sub-plots. The nodulation, plant biomass, and grain yield were evaluated, and in some experiments the BNF contribution by 15N natural abundance technique and ureides sap content were also assessed. There were differences between genotypes and regions. All genotypes had nodules even under mineral N. The most efficient genotypes in nodulation and in obtaining N by biological fixation were CNF 0011234, CNF 0011028, CNF 0011559 and PI 387865, Andean genotypes, and CNF 0011075, CNF 0011137, CNF 0011240, PI 325750, Mesoamerican genotypes. The natural abundance of 15N technique showed that the Mesoamerican genotypes had higher contribution of N2 fixation. Most genotypes showed similar grain yields to commercial cultivars. The most pronounced differences between the genotypes and cultivars were detected in the experiment in GO in 2013 winter and the greatest similarities in 2014 winter. Most of the genotypes did not have grain yield differences between the two N sources. The fertilized genotypes had grain yields ranging from 167 to 3567 kg ha-1 and inoculated genotypes ranging from 180 to 3307 kg ha-1. In both cases, the lowest values of grain yield were found in PR, and the highest values in the winter of 2014 in GO, reflecting the average yield obtained in each location. |
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Ara?jo, Adelson Paulo de585.444.029-68Ferreira, Enderson Petr?nio de Brito693.429.465-34Ara?jo, Adelson Paulo deAbboud, Antonio Carlos de SouzaMercante, F?bio MartinsAra?jo, Jean Luiz Sim?es deStraliotto, Ros?ngela047.979.217-81http://lattes.cnpq.br/2952293856326556Knupp, Adriano Moreira2019-08-16T17:00:31Z2015-02-25KNUPP, Adriano Moreira. Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio. 2015. 119 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015.https://tede.ufrrj.br/jspui/handle/jspui/2818The use of the diversity of common bean (Phaseolus vulgaris L.) germplasm collections can provide materials with higher contribution of biological nitrogen fixation. The aim of this study was to identify, in germplasm bank of Embrapa Rice and Beans, common bean genotypes of Mesoamerican and Andean origin with potential for obtaining N2 by rhizobia inoculation. The evaluation consisted of two screenings of 879 genotypes in greenhouse. Further, 15 of these genotypes and two commercial cultivars were evaluated under field conditions. Greenhouse experiments were conducted in randomized block design with three replications in which 879 genotypes were evaluated in response to inoculation with commercial Rhizobium strains (SEMIA 4077, SEMIA 4080, SEMIA 4088). The cultivar Ouro Negro was used as reference. Pre-germinated seeds were seeded in 3 L pots with sterile sand and vermiculite. Plants were inoculated seven days after emergence (DAE), and sterile Norris solution without N was added weekly. The number of nodules, nodule dry weight and one nodule dry weight were determined 35 DAE. The percentage of genotypes that exceeded the reference was established, as 21% for number of nodules, 46% for nodule dry weight and 32% for one nodule dry weight. We selected 116 genotypes, which were evaluated in other experiment. A joint analysis of the data obtained in the first and second phases for these 116 genotypes was performed, and 15 genotypes were selected. These 15 genotypes and the commercial cultivars P?rola and Ouro Negro were evaluated at field conditions under two N sources, inoculation with rhizobia or fertilization with 80 kg N ha-1. Five experiments were carried out in Santo Ant?nio de Goi?s ? GO, Ponta Grossa ? PR and Serop?dica ? RJ. A split plot design in randomized complete block was used with N sources as main plots and genotypes as sub-plots. The nodulation, plant biomass, and grain yield were evaluated, and in some experiments the BNF contribution by 15N natural abundance technique and ureides sap content were also assessed. There were differences between genotypes and regions. All genotypes had nodules even under mineral N. The most efficient genotypes in nodulation and in obtaining N by biological fixation were CNF 0011234, CNF 0011028, CNF 0011559 and PI 387865, Andean genotypes, and CNF 0011075, CNF 0011137, CNF 0011240, PI 325750, Mesoamerican genotypes. The natural abundance of 15N technique showed that the Mesoamerican genotypes had higher contribution of N2 fixation. Most genotypes showed similar grain yields to commercial cultivars. The most pronounced differences between the genotypes and cultivars were detected in the experiment in GO in 2013 winter and the greatest similarities in 2014 winter. Most of the genotypes did not have grain yield differences between the two N sources. The fertilized genotypes had grain yields ranging from 167 to 3567 kg ha-1 and inoculated genotypes ranging from 180 to 3307 kg ha-1. In both cases, the lowest values of grain yield were found in PR, and the highest values in the winter of 2014 in GO, reflecting the average yield obtained in each location.O uso da diversidade em cole??es de germoplasma de feijoeiro comum (Phaseolus vulgaris L.) pode prover materiais com maior contribui??o da fixa??o biol?gica de nitrog?nio. O objetivo deste trabalho foi identificar gen?tipos de feijoeiro, de origem mesoamericana e andina, provenientes do banco de germoplasma da Embrapa Arroz e Feij?o, com potencial de obten??o de N2 a partir da inocula??o com riz?bios. A avalia??o consistiu de duas triagens de 879 gen?tipos em casa de vegeta??o, e posteriormente quinze destes gen?tipos e duas cultivares comerciais foram avaliados em campo. Os experimentos em casa de vegeta??o possu?am delineamento em blocos casualizados com tr?s repeti??es, nos quais foram avaliados 879 gen?tipos em resposta ? inocula??o com estirpes comerciais de Rhizobium (SEMIA 4077, SEMIA 4080, SEMIA 4088). A cultivar Ouro Negro foi utilizada como refer?ncia, e sementes pr?-germinadas foram semeadas em potes (3 L) com areia e vermiculita est?reis. As plantas foram inoculadas sete dias ap?s emerg?ncia (DAE), e solu??o de Norris sem N era adicionada semanalmente. Aos 35 DAE, foram determinados o n?mero de n?dulos, massa seca de n?dulos e massa seca de um n?dulo. Os percentuais de gen?tipos que superaram a refer?ncia foram de 21% em n?mero de n?dulos, 46% em massa de n?dulos e 32% em massa de um n?dulo. Foram selecionados 116 gen?tipos, que foram avaliados em novo experimento. Ap?s an?lise conjunta dos dados obtidos na primeira e segunda fases para esses 116 gen?tipos, quinze gen?tipos foram selecionados. Estes quinze gen?tipos e as cultivares P?rola e Ouro Negro foram avaliados sob duas fontes de N, inocula??o com riz?bios ou fertiliza??o com 80 kg ha-1 de N. Cinco experimentos foram conduzidos em Santo Ant?nio de Goi?s (GO), Ponta Grossa (PR), e Serop?dica (RJ), em delineamento em blocos ao acaso em parcelas subdivididas, com as fontes de N dispostas nas parcelas e os gen?tipos nas subparcelas. Foram avaliadas nodula??o, biomassa e produtividade, e em alguns experimentos a contribui??o da FBN via abund?ncia natural de 15N e os teores de N-ure?dos na seiva. Houve diferen?as de desempenho entre gen?tipos e entre locais. Todos gen?tipos nodularam, mesmo quando receberam N mineral. Os mais eficientes na nodula??o e na obten??o de N via FBN foram CNF 0011234, CNF 0011028, CNF 0011559 e PI 387865, andinos, e CNF 0011075, CNF 0011137, CNF 0011240, PI 325750, mesoamericanos. A abund?ncia natural de 15N demonstrou que os gen?tipos mesoamericanos apresentaram maior contribui??o da fixa??o do N2. A maioria dos gen?tipos apresentou produtividades de gr?os semelhantes ?s cultivares comerciais. As diferen?as mais pronunciadas entre os gen?tipos e as cultivares foram detectadas no experimento de GO, no inverno de 2013, e as maiores semelhan?as no inverno de 2014. A maioria dos gen?tipos n?o apresentou diferen?a de produtividade entre as duas fontes de N. Os gen?tipos sob aduba??o tiveram produtividades que variaram de 167 a 3567 kg ha-1 e sob inocula??o de 180 a 3307 kg ha-1. Em ambas as fontes de N, as menores produtividades foram verificadas em PR as maiores em GO no inverno de 2014, refletindo a m?dia geral obtida em cada localidade.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2019-08-16T17:00:31Z No. of bitstreams: 1 2015 - Adriano Moreira Knupp.pdf: 4070462 bytes, checksum: b203a6a19b7184bd241f2b9263d2681c (MD5)Made available in DSpace on 2019-08-16T17:00:31Z (GMT). No. of bitstreams: 1 2015 - Adriano Moreira Knupp.pdf: 4070462 bytes, checksum: b203a6a19b7184bd241f2b9263d2681c (MD5) Previous issue date: 2015-02-25application/pdfhttps://tede.ufrrj.br/retrieve/10318/2015%20-%20Adriano%20Moreira%20Knupp.pdf.jpghttps://tede.ufrrj.br/retrieve/15934/2015%20-%20Adriano%20Moreira%20Knupp.pdf.jpghttps://tede.ufrrj.br/retrieve/22240/2015%20-%20Adriano%20Moreira%20Knupp.pdf.jpghttps://tede.ufrrj.br/retrieve/28622/2015%20-%20Adriano%20Moreira%20Knupp.pdf.jpghttps://tede.ufrrj.br/retrieve/35056/2015%20-%20Adriano%20Moreira%20Knupp.pdf.jpghttps://tede.ufrrj.br/retrieve/41506/2015%20-%20Adriano%20Moreira%20Knupp.pdf.jpghttps://tede.ufrrj.br/retrieve/47860/2015%20-%20Adriano%20Moreira%20Knupp.pdf.jpghttps://tede.ufrrj.br/retrieve/54352/2015%20-%20Adriano%20Moreira%20Knupp.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Agronomia - Ci?ncia do SoloUFRRJBrasilInstituto de AgronomiaDiversidadeMelhoramento de plantasFixa??o biol?gica de nitrog?nioDiversityPlant breedingBiological nitrogen fixationAgronomiaSele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bioSelection of mesoamerican and andean common bean genotypes in response to rhizobial inoculationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2015 - Adriano Moreira Knupp.pdf.jpg2015 - Adriano Moreira Knupp.pdf.jpgimage/jpeg3969http://localhost:8080/tede/bitstream/jspui/2818/18/2015+-+Adriano+Moreira+Knupp.pdf.jpge54fe07ecb50bd588279af9311c4769fMD518TEXT2015 - Adriano Moreira Knupp.pdf.txt2015 - Adriano Moreira Knupp.pdf.txttext/plain389864http://localhost:8080/tede/bitstream/jspui/2818/17/2015+-+Adriano+Moreira+Knupp.pdf.txt921b83d4bded5487fc6047ecf3f67949MD517ORIGINAL2015 - Adriano Moreira Knupp.pdf2015 - Adriano Moreira Knupp.pdfapplication/pdf4070462http://localhost:8080/tede/bitstream/jspui/2818/2/2015+-+Adriano+Moreira+Knupp.pdfb203a6a19b7184bd241f2b9263d2681cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82089http://localhost:8080/tede/bitstream/jspui/2818/1/license.txt7b5ba3d2445355f386edab96125d42b7MD51jspui/28182023-02-15 16:16:12.216oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2023-02-15T18:16:12Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio |
dc.title.alternative.eng.fl_str_mv |
Selection of mesoamerican and andean common bean genotypes in response to rhizobial inoculation |
title |
Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio |
spellingShingle |
Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio Knupp, Adriano Moreira Diversidade Melhoramento de plantas Fixa??o biol?gica de nitrog?nio Diversity Plant breeding Biological nitrogen fixation Agronomia |
title_short |
Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio |
title_full |
Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio |
title_fullStr |
Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio |
title_full_unstemmed |
Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio |
title_sort |
Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio |
author |
Knupp, Adriano Moreira |
author_facet |
Knupp, Adriano Moreira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ara?jo, Adelson Paulo de |
dc.contributor.advisor1ID.fl_str_mv |
585.444.029-68 |
dc.contributor.advisor-co1.fl_str_mv |
Ferreira, Enderson Petr?nio de Brito |
dc.contributor.advisor-co1ID.fl_str_mv |
693.429.465-34 |
dc.contributor.referee1.fl_str_mv |
Ara?jo, Adelson Paulo de |
dc.contributor.referee2.fl_str_mv |
Abboud, Antonio Carlos de Souza |
dc.contributor.referee3.fl_str_mv |
Mercante, F?bio Martins |
dc.contributor.referee4.fl_str_mv |
Ara?jo, Jean Luiz Sim?es de |
dc.contributor.referee5.fl_str_mv |
Straliotto, Ros?ngela |
dc.contributor.authorID.fl_str_mv |
047.979.217-81 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2952293856326556 |
dc.contributor.author.fl_str_mv |
Knupp, Adriano Moreira |
contributor_str_mv |
Ara?jo, Adelson Paulo de Ferreira, Enderson Petr?nio de Brito Ara?jo, Adelson Paulo de Abboud, Antonio Carlos de Souza Mercante, F?bio Martins Ara?jo, Jean Luiz Sim?es de Straliotto, Ros?ngela |
dc.subject.por.fl_str_mv |
Diversidade Melhoramento de plantas Fixa??o biol?gica de nitrog?nio |
topic |
Diversidade Melhoramento de plantas Fixa??o biol?gica de nitrog?nio Diversity Plant breeding Biological nitrogen fixation Agronomia |
dc.subject.eng.fl_str_mv |
Diversity Plant breeding Biological nitrogen fixation |
dc.subject.cnpq.fl_str_mv |
Agronomia |
description |
The use of the diversity of common bean (Phaseolus vulgaris L.) germplasm collections can provide materials with higher contribution of biological nitrogen fixation. The aim of this study was to identify, in germplasm bank of Embrapa Rice and Beans, common bean genotypes of Mesoamerican and Andean origin with potential for obtaining N2 by rhizobia inoculation. The evaluation consisted of two screenings of 879 genotypes in greenhouse. Further, 15 of these genotypes and two commercial cultivars were evaluated under field conditions. Greenhouse experiments were conducted in randomized block design with three replications in which 879 genotypes were evaluated in response to inoculation with commercial Rhizobium strains (SEMIA 4077, SEMIA 4080, SEMIA 4088). The cultivar Ouro Negro was used as reference. Pre-germinated seeds were seeded in 3 L pots with sterile sand and vermiculite. Plants were inoculated seven days after emergence (DAE), and sterile Norris solution without N was added weekly. The number of nodules, nodule dry weight and one nodule dry weight were determined 35 DAE. The percentage of genotypes that exceeded the reference was established, as 21% for number of nodules, 46% for nodule dry weight and 32% for one nodule dry weight. We selected 116 genotypes, which were evaluated in other experiment. A joint analysis of the data obtained in the first and second phases for these 116 genotypes was performed, and 15 genotypes were selected. These 15 genotypes and the commercial cultivars P?rola and Ouro Negro were evaluated at field conditions under two N sources, inoculation with rhizobia or fertilization with 80 kg N ha-1. Five experiments were carried out in Santo Ant?nio de Goi?s ? GO, Ponta Grossa ? PR and Serop?dica ? RJ. A split plot design in randomized complete block was used with N sources as main plots and genotypes as sub-plots. The nodulation, plant biomass, and grain yield were evaluated, and in some experiments the BNF contribution by 15N natural abundance technique and ureides sap content were also assessed. There were differences between genotypes and regions. All genotypes had nodules even under mineral N. The most efficient genotypes in nodulation and in obtaining N by biological fixation were CNF 0011234, CNF 0011028, CNF 0011559 and PI 387865, Andean genotypes, and CNF 0011075, CNF 0011137, CNF 0011240, PI 325750, Mesoamerican genotypes. The natural abundance of 15N technique showed that the Mesoamerican genotypes had higher contribution of N2 fixation. Most genotypes showed similar grain yields to commercial cultivars. The most pronounced differences between the genotypes and cultivars were detected in the experiment in GO in 2013 winter and the greatest similarities in 2014 winter. Most of the genotypes did not have grain yield differences between the two N sources. The fertilized genotypes had grain yields ranging from 167 to 3567 kg ha-1 and inoculated genotypes ranging from 180 to 3307 kg ha-1. In both cases, the lowest values of grain yield were found in PR, and the highest values in the winter of 2014 in GO, reflecting the average yield obtained in each location. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-25 |
dc.date.accessioned.fl_str_mv |
2019-08-16T17:00:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
KNUPP, Adriano Moreira. Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio. 2015. 119 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/2818 |
identifier_str_mv |
KNUPP, Adriano Moreira. Sele??o de gen?tipos mesoamericanos e andinos de feijoeiro em resposta ? inocula??o com riz?bio. 2015. 119 f. Tese (Doutorado em Agronomia, Ci?ncia do Solo). Instituto de Agronomia, Departamento de Solos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015. |
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https://tede.ufrrj.br/jspui/handle/jspui/2818 |
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Universidade Federal Rural do Rio de Janeiro |
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Programa de P?s-Gradua??o em Agronomia - Ci?ncia do Solo |
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UFRRJ |
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Brasil |
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Instituto de Agronomia |
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Universidade Federal Rural do Rio de Janeiro |
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